Metabolic Efficiency of Tetrahymena pyriformis, Strain W, as Related to Culture Age

Hull, Robert W.; Morrissey, JH

doi:10.2307/3224078

Cited by 5 publications

(1 citation statement)

References 2 publications

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“…For Tetrahymena, in particular. the influence of age of culture ( 12,22.3 1 ) , oxygen tension ( 2 1 ) starvation(32) , and temperature ( 14,33) have been noted to alter the size or volume, and some of these factors also have been correlated with altered metabolic activity. Further, it has been demonstrated that composition of the culture medium has an influence on several areas of metabolism ( 29 ) .…”

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Iron Deficiency and the Metabolism of *Tetrahymena pyriformis**

Conner

Cline

1964

The Journal of Protozoology

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SYNOPSIS. An iron deficiency was noted for Tetrahymena grown in a proteose‐peptone‐yeast extract medium. Thus, the addition of an iron chelate and glucose was found to give consistent and luxuriant growth, whereas a number of other additives, including sodium acetate, an inorganic salt mixture lacking iron, and vitamins were found to be without influence on growth rate or cellular yield. Iron‐deficient cells showed a marked reduction of oxidative capacity when suspended in a non‐nutrient medium as well as when supplied with oxidizable substrates. A 1 hour incubation of iron‐deficient Tetrahymena with iron chelate restored oxidative capacity only when the cells were suspended in a nutrient (peptone) medium. Growth of Tetrahymena in media autoclaved with salts resulted in cells with a larger volume but with no increase in oxidative capacity. The addition of 2,4‐dinitrophenol elevated respiration of cells suspended in a non‐nutrient medium, and of iron deficient cells suspended in the growth medium, suggesting that this compound increases the availability of iron or iron containing compounds, or by‐passes the limiting steps. Respiratory quotients were lowered by an iron deficiency and elevated when the cells were grown in the presence of iron and glucose. This suggests more complete utilization of the carbohydrate, possibly due to the activity of a number of enzymes known to be iron‐containing metalloproteins that are required for oxidative glucose dissimilation.

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Iron Deficiency and the Metabolism of *Tetrahymena pyriformis**

Conner

Cline

1964

The Journal of Protozoology

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On cell organelles in Tetrahymena. With special reference to mitochondria and peroxisomes

Nilsson

1981

Carlsberg Res. Commun.

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In the ciliate, Tetrahymena pyriformis, the population of cell organelles shows variation during the culture cycle and when the cells are subjected to various treatments. New cell constituents may appear and the fine structure of mitochondria and peroxisomes may change. Such dynamic structural changes are described and reviewed in relation to physiological and biochemical studies on Tetrahymena. The cell organelles discussed are about I tam in diameter or smaller~ reference is also made to the nuc[eotar organization which is the most sensitive indicator of the physiologica~ state of the ceils. The presumed distribution of the various cell organelles in subcellular fractions of Tetrahymena is mentioned, since dependent on the pretreatment of the cells, especially the mitochondrial and peroxisomal fractions may be contaminated to varying degrees with other cell organelles~The purpose of the review is to demonstrate that a correlation exists between the structure and function of cell organelles, especially mitochondria and peroxisomes, and that the overall fine structure of a cell reflects its physiological state.

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