Metabolic engineering and synthetic biology of plant natural products – A minireview

Birchfield, Aaron; McIntosh, Cecilia A.

doi:10.1016/j.cpb.2020.100163

Cited by 66 publications

(38 citation statements)

References 120 publications

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“…Malonyl-CoA is mainly produced by acetyl-CoA via the catalysis of acetyl-CoA carboxylase (ACC) [7]. Subsequently, the chalcone backbone is catalyzed by different classes of enzymes (such as isomerase, hydroxylase, oxido-reductase, transferase) to produce other flavonoid subgroups [8]. Chalcones can be converted into flavanones by chalcone isomerase (CHI) or to aurones by plant polyphenol oxidase (PPO) [8].…”

Section: Biosynthesis Of Flavonoids In Plantsmentioning

confidence: 99%

“…Subsequently, the chalcone backbone is catalyzed by different classes of enzymes (such as isomerase, hydroxylase, oxido-reductase, transferase) to produce other flavonoid subgroups [8]. Chalcones can be converted into flavanones by chalcone isomerase (CHI) or to aurones by plant polyphenol oxidase (PPO) [8]. However, flavanones can not only be hydroxylated by flavanone 3−OH transferase (F3H) to produce flavanonols but can also be converted into flavones with a double bond between the C-2 and C-3 (of the C-ring) through the enzymatic action of flavone synthase (FNS) [58].…”

Section: Biosynthesis Of Flavonoids In Plantsmentioning

confidence: 99%

“…In plants, flavonoids are responsible for flower color and aroma and attract pollinators to disperse fruit and help plants propagate [6]. In addition, they also help plants combat various biotic and abiotic stresses, including ultraviolet protection in leaves, protection from microbial infections and herbivores, and defending from physical and radical damage [7,8]. As a kind of phytochemicals, flavonoids cannot be synthesized by humans or animals.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic Engineering of Microbial Cell Factories for Biosynthesis of Flavonoids: A Review

Lou

Hu²,

et al. 2021

Molecules

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Flavonoids belong to a class of plant secondary metabolites that have a polyphenol structure. Flavonoids show extensive biological activity, such as antioxidative, anti-inflammatory, anti-mutagenic, anti-cancer, and antibacterial properties, so they are widely used in the food, pharmaceutical, and nutraceutical industries. However, traditional sources of flavonoids are no longer sufficient to meet current demands. In recent years, with the clarification of the biosynthetic pathway of flavonoids and the development of synthetic biology, it has become possible to use synthetic metabolic engineering methods with microorganisms as hosts to produce flavonoids. This article mainly reviews the biosynthetic pathways of flavonoids and the development of microbial expression systems for the production of flavonoids in order to provide a useful reference for further research on synthetic metabolic engineering of flavonoids. Meanwhile, the application of co-culture systems in the biosynthesis of flavonoids is emphasized in this review.

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Section: Biosynthesis Of Flavonoids In Plantsmentioning

confidence: 99%

Section: Biosynthesis Of Flavonoids In Plantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolic Engineering of Microbial Cell Factories for Biosynthesis of Flavonoids: A Review

Lou

Hu²,

et al. 2021

Molecules

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“…In all the synthetic biology strategies, omics databases and computational tools for pathway simulations have been developed to offer the possibility of using promiscuous enzymatic capabilities for maximizing the yields of desired molecules [ 129 , 133 ]. However, a major hurdle in microbial platforms was the incomplete knowledge of a biosynthetic pathway leading to plant specialized metabolites.…”

Section: Novel Perspectives In the Production Of Plant-derived Compoundsmentioning

confidence: 99%

Specialized Metabolites and Valuable Molecules in Crop and Medicinal Plants: The Evolution of Their Use and Strategies for Their Production

D’Amelia

Docimo

Crocoll

et al. 2021

Genes

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Plants naturally produce a terrific diversity of molecules, which we exploit for promoting our overall well-being. Plants are also green factories. Indeed, they may be exploited to biosynthesize bioactive molecules, proteins, carbohydrates and biopolymers for sustainable and large-scale production. These molecules are easily converted into commodities such as pharmaceuticals, antioxidants, food, feed and biofuels for multiple industrial processes. Novel plant biotechnological, genetics and metabolic insights ensure and increase the applicability of plant-derived compounds in several industrial sectors. In particular, synergy between disciplines, including apparently distant ones such as plant physiology, pharmacology, ‘omics sciences, bioinformatics and nanotechnology paves the path to novel applications of the so-called molecular farming. We present an overview of the novel studies recently published regarding these issues in the hope to have brought out all the interesting aspects of these published studies.

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“…Several platforms, including bacteria ( Farrokh et al, 2019 ), yeast ( Rahmat and Kang, 2020 ), algae ( Rico et al, 2017 ), and plants ( Birchfield and McIntosh, 2020 ), have been successfully used for enhanced (or mass) production of specific isoprenoids of industrial value. Though traditionally microbial systems have been the platform of choice for the production of recombinant natural products, plants are emerging as important alternatives.…”

Section: Engineering Isoprenoid Biosynthesis In Trichomesmentioning

confidence: 99%

Isoprenoid Metabolism and Engineering in Glandular Trichomes of Lamiaceae

2021

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The isoprenoids play important ecological and physiological roles in plants. They also have a tremendous impact on human lives as food additives, medicines, and industrial raw materials, among others. Though some isoprenoids are highly abundant in nature, plants produce many at extremely low levels. Glandular trichomes (GT), which cover the aerial parts of more than 25% of vascular plants, have been considered as natural biofactories for the mass production of rare industrially important isoprenoids. In several plant genera (e.g., Lavandula and Mentha), GTs produce and store large quantities of the low molecular weight isoprenoids, in particular mono- and sesquiterpenes, as essential oil constituents. Within each trichome, a group of secretory cells is specialized to strongly and specifically express isoprenoid biosynthetic genes, and to synthesize and deposit copious amounts of terpenoids into the trichome’s storage reservoir. Despite the abundance of certain metabolites in essential oils and defensive resins, plants, particularly those lacking glandular trichomes, accumulate small quantities of many of the biologically active and industrially important isoprenoids. Therefore, there is a pressing need for technologies to enable the mass production of such metabolites, and to help meet the ever-increasing demand for plant-based bioproducts, including medicines and renewable materials. Considerable contemporary research has focused on engineering isoprenoid metabolism in GTs, with the goal of utilizing them as natural biofactories for the production of valuable phytochemicals. In this review, we summarize recent advances related to the engineering of isoprenoid biosynthetic pathways in glandular trichomes.

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Metabolic engineering and synthetic biology of plant natural products – A minireview

Cited by 66 publications

References 120 publications

Metabolic Engineering of Microbial Cell Factories for Biosynthesis of Flavonoids: A Review

Metabolic Engineering of Microbial Cell Factories for Biosynthesis of Flavonoids: A Review

Specialized Metabolites and Valuable Molecules in Crop and Medicinal Plants: The Evolution of Their Use and Strategies for Their Production

Isoprenoid Metabolism and Engineering in Glandular Trichomes of Lamiaceae

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