Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds by Corynebacterium glutamicum

Wendisch, Volker F.; Nampoothiri, K. Madhavan; Lee, Jinho

doi:10.3389/fmicb.2022.835131

Cited by 15 publications

(9 citation statements)

References 142 publications

(217 reference statements)

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“…A plethora of metabolic pathways for breakdown and assimilation of aromatic compounds are known in C. glutamicum , which guided the biosynthesis of versatile aromatic compounds by interception and/or extension of these pathways ( Brinkrolf et al, 2006 ; Shen et al, 2012 ; Lee and Wendisch, 2017 ). Thus, this bacterium has been used a prominent host for the production of aromatic compounds, such as l -tryptophan, halogenated l -tryptophans, anthranilate, methylated anthranilates, 4-hydroxybenzoate (4-HBA), 4-aminobenzoate, protocatechuate (PCA), indole-3-acetic acid, violacein, and anthocyanin ( Lee and Wendisch, 2017 ; Kim et al, 2019 ; Wendisch et al, 2022 ). Recently, we engineered C. glutamicum to produce 19 g/L of 4-HBA in a 5-L bioreactor ( Syukur Purwanto et al, 2018 ), and, by extension of this concept, introduction of CAR enabled production of 2.3 g/L of 4-HB alcohol as a main product and 0.3 g/L of 4-HB aldehyde as a minor by-product in flask culture ( Kim et al, 2020 ; Figure 1A ).…”

Section: Introductionmentioning

confidence: 99%

Engineered Corynebacterium glutamicum as the Platform for the Production of Aromatic Aldehydes

Kim

Choi²,

Kim³

et al. 2022

Front. Bioeng. Biotechnol.

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Section: Introductionmentioning

confidence: 99%

Engineered Corynebacterium glutamicum as the Platform for the Production of Aromatic Aldehydes

Kim

Choi²,

Kim³

et al. 2022

Front. Bioeng. Biotechnol.

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“…Here, we demonstrated the successful production of CoQ 10 from a wheat side stream-based hydrolysate that has been utilized previously for the production of 5-aminovalerate [ 40 ] and l -2-hydroxyglutarate [ 22 ]. As well as the wheat side stream, access to numerous monomeric and polymeric carbon sources has been established, e.g., xylose, arabinose, mannose, starch, lignocellulose, N -acetylglucosamine, and alginate, which can be derived from hydrolysates of sustainable second generation feedstocks such as spent sulfite liquor, Miscanthus biomass, brown seaweed, corn straw, rice straw, and shrimp waste [ 63 , 64 , 65 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production

et al. 2022

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Coenzyme Q10 (CoQ10) is a lipid-soluble compound with important physiological functions and is sought after in the food and cosmetic industries owing to its antioxidant properties. In our previous proof of concept, we engineered for CoQ10 biosynthesis the industrially relevant Corynebacterium glutamicum, which does not naturally synthesize any CoQ. Here, liquid chromatography–mass spectrometry (LC–MS) analysis identified two metabolic bottlenecks in the CoQ10 production, i.e., low conversion of the intermediate 10-prenylphenol (10P-Ph) to CoQ10 and the accumulation of isoprenologs with prenyl chain lengths of not only 10, but also 8 to 11 isopentenyl units. To overcome these limitations, the strain was engineered for expression of the Ubi complex accessory factors UbiJ and UbiK from Escherichia coli to increase flux towards CoQ10, and by replacement of the native polyprenyl diphosphate synthase IspB with a decaprenyl diphosphate synthase (DdsA) to select for prenyl chains with 10 isopentenyl units. The best strain UBI6-Rs showed a seven-fold increased CoQ10 content and eight-fold increased CoQ10 titer compared to the initial strain UBI4-Pd, while the abundance of CoQ8, CoQ9, and CoQ11 was significantly reduced. This study demonstrates the application of the recent insight into CoQ biosynthesis to improve metabolic engineering of a heterologous CoQ10 production strain.

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“…Corynebacterium glutamicum is the workhorse for the production of a plethora of amino acids, amines, alcohols, organic acids, and aromatic compounds [ 28 , 29 , 60 , 61 ]. l -Tryptophan production in C. glutamicum was enabled by plasmid-borne expression of trpD from Escherichia coli and genome-based expression of both aroG FBR from E. coli and trpE FBR , together with the deletion of csm encoding chorismate mutase [ 57 ] (Fig.…”

Section: Introductionmentioning

confidence: 99%

Fermentative aminopyrrolnitrin production by metabolically engineered Corynebacterium glutamicum

Putri,

Jung,

Lee

et al. 2024

Microb Cell Fact

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Aminopyrrolnitrin (APRN), a natural halogenated phenylpyrrole derivative (HPD), has strong antifungal and antiparasitic activities. Additionally, it showed 2.8-fold increased photostability compared to pyrrolnitrin, a commercially available HPD with antimicrobial activity. For microbial production of APRN, we first engineered anthranilate phosphoribosyltransferase encoded by trpD from Corynebacterium glutamicum, resulting in a TrpDA162D mutation that exhibits feedback-resistant against l-tryptophan and higher substrate affinity compared to wild-type TrpD. Plasmid-borne expression of trpDA162D in C. glutamicum TP851 strain with two copies of trpDA162D in the genome led to the production of 3.1 g/L l-tryptophan in flask culture. Subsequent step for l-tryptophan chlorination into 7-chloro-l-tryptophan was achieved by introducing diverse sources of genes encoding tryptophan 7-halogenase (PrnA or RebH) and flavin reductase (Fre, PrnF, or RebF). The combined expression of prnA from Serratia grimesii or Serratia plymuthica with flavin reductase gene from Escherichia coli, Pseudomonas fluorescens, or Lechevalieria aerocolonigenes yielded higher production of 7-chloro-l-tryptophan in comparison to other sets of two-component systems. In the next step, production of putative monodechloroaminopyrrolnitrin (MDAP) from 7-chloro-l-tryptophan was achieved through the expression of prnB encoding MDAP synthase from S. plymuthica or P. fluorescens. Finally, an artificial APRN biosynthetic pathway was constructed by simultaneously expressing genes coding for tryptophan 7-halogenase, flavin reductase, MDAP synthase, and MDAP halogenase (PrnC) from different microbial sources within the l-tryptophan-producing TP851 strain. As prnC from S. grimesii or S. plymuthica was introduced into the host strain, which carried plasmids expressing prnA from S. plymuthica, fre from E. coli, and prnB from S. plymuthica, APN3639 and APN3638 accumulated 29.5 mg/L and 28.1 mg/L of APRN in the culture broth. This study represents the first report on the fermentative APRN production by metabolically engineered C. glutamicum.

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Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds by Corynebacterium glutamicum

Abstract: Graphical AbstractStrategy to valorize non-utilized nitrogen from sidestreams by producing nitrogen-containing products.

Cited by 15 publications

References 142 publications

Engineered Corynebacterium glutamicum as the Platform for the Production of Aromatic Aldehydes

Engineered Corynebacterium glutamicum as the Platform for the Production of Aromatic Aldehydes

Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production

Fermentative aminopyrrolnitrin production by metabolically engineered Corynebacterium glutamicum

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