Metabolic engineering in hairy roots: An outlook on production of plant secondary metabolites

Bagal, Diksha; Chowdhary, Aksar Ali; Mehrotra, Shakti; Mishra, Sonal; Rathore, Surbhi; Srivastava, Vikas

doi:10.1016/j.plaphy.2023.107847

Cited by 17 publications

(4 citation statements)

References 110 publications

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“…Plants serve as a significant source of secondary metabolites, which possess diverse roles in human welfare, including therapeutic implications. However, the yield of secondary metabolites obtained from natural populations of plants is insufficient to meet commercial demands due to their limited accumulation [81]. Various in vitro culture techniques, including callus, hairy root, shoot, and suspension cultures, can be utilized to enhance the biosynthesis of specific metabolites in commercially important plants [82].…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Culture Conditions on Anthocyanins and Related Genes in Red Pear Callus

Yao,

Lei,

Zhou

et al. 2023

Agronomy

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Red pears are appreciated for their abundant nutritional benefits and visually striking red hue, rendering them a favored option among consumers and stimulating substantial market demand. The present study employs the flesh of a red pear as the explant, subjecting the flesh callus to varying sugar sources, MS concentrations, light qualities, and temperatures to investigate the alterations in secondary metabolites, including anthocyanins, within the callus. It was found that sucrose can induce more anthocyanins, and its related metabolites and genes also increase as the sucrose and MS concentrations increase. Under the conditions of red-blue light and a temperature of 15 °C, it can further induce the production of more anthocyanins and secondary metabolites and can also upregulate the synthesis of anthocyanin-related genes. As such, this investigation serves to elucidate the factors that contribute to anthocyanin accumulation in red pears, thereby providing a theoretical foundation for understanding the mechanisms underlying color change.

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Section: Discussionmentioning

confidence: 99%

Effect of Different Culture Conditions on Anthocyanins and Related Genes in Red Pear Callus

Yao,

Lei,

Zhou

et al. 2023

Agronomy

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“…Agrobacterium rhizogenes is a gram-negative soil-borne bacterium, that can infect several plant species at the wounding site and their infections lead to the development of adventitious roots with multiple hairy roots (Mauro & Bettini, 2021;Stepanova et al, 2022). Hairy roots developed through A. rhizogenes transformation exhibit rapid growth rates, genetic stability, and possess the ability to produce useful natural compounds at comparable levels to those of wild-type roots, these hairy root cultures are attractive for the synthesis of secondary metabolites (Malarz et al, 2022;Bagal et al, 2023;Biswas et al, 2023). The secondary metabolites production in the hairy root cultures of various plants is influenced by several variables, such as light, temperature, medium composition, pH,…”

Section: Introductionmentioning

confidence: 99%

Influence of cytokinins and yeast extract on growth and flavone production in hairy root cultures of Scutellaria baicalensis

Kwon,

Kim,

Choi

et al. 2023

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Hairy roots produce various bioactive chemical compounds than wild-type roots which offer a promising in vitro approach for synthesizing important nutraceutical compounds. The purpose of this study is to increase the production of flavone compounds in hairy root cultures of Scutellaria baicalensis by the addition of elicitors such as cytokinins and yeast extract. Cytokinins such as kinetin (KIN), 6-benzylaminopurine (BAP), and Thidiazuron (TDZ) were utilized at 0.1, 0.5, and 1.0 mg/L, whereas for yeast extract treatment 50, 100, and 150 mg/L concentrations were added to the ½ SH medium. Effects of elicitors were measured in terms of dry biomass and flavone contents (baicalin, baicalein, and wogonin) using high-performance liquid chromatography (HPLC). The highest dry weight was achieved in the control hairy root than that of all cytokinins-treated hairy root cultures. In all the cytokinin-treated hairy root cultures, as the concentration increased the dry weight of the hairy root decreased. In contrast, in all the yeast extract-treated hairy root cultures as the concentration increases the dry weight of the hairy root increased, whereas the highest dry weight was achieved in 150 mg/L of yeast extract. Moving to the flavone content, baicalin was detected highest content in all the hairy root cultures supplied with cytokinin and yeast extract. The highest total flavone content was achieved in the hairy root culture treated with 1.0 mg/L of TDZ and 50 mg/L of yeast extract. This result might help the commercial agronomic sector by facilitating the in vitro mass production of nutraceuticals using S. baicalensis hairy root cultures.

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“…For these reasons, hairy roots have been considered an appropriate tool for substituting microorganisms, including Saccharomyces cerevisiae and Escherichia coli, for natural plant metabolite biosynthesis because they are closer to the native host plant [16]. In many studies, the hairy root culture with diverse plants has been used to enhance phytochemical production [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia

Choi,

Yoon,

Yang

et al. 2023

Plants

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Salvia plebeia (Lamiaceae) is a medicinal plant containing diverse bioactive constituents that have biological properties. In this study, we determined the optimal conditions (media and auxin) for the hairy root culture of S. plebeia for the growth and accumulation of phenolic compounds and evaluated its antioxidant activities. Rosmarinic acid and five phenylpropanoids were detected using high-performance liquid chromatography. The hairy roots grown in 1/2 SH medium with 1 mg/L NAA had a high level of rosmarinic acid content. Hairy roots cultured in 1 mg/L NAA had the highest total content of five phenylpropanoids. Compared to wild-type roots grown in the field, hairy roots (NAA 1) expressed similar levels of rosmarinic acid but significantly enhanced phenylpropanoid accumulation. Furthermore, the total phenolic content and total flavonoid content of hairy roots (NAA 1) were 2.22 and 1.73 times higher than those of wild-type roots. In the results of DPPH, ABTS, and reducing power assays, the hairy roots (NAA 1) showed higher free radical scavenging effects and reduction potential than the wild-type roots. These results suggest that S. plebeia hairy roots cultured under optimal conditions, which exhibit enhanced phenolic compound accumulation and antioxidant activity, can potentially be used as sources of antioxidants.

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Metabolic engineering in hairy roots: An outlook on production of plant secondary metabolites

Cited by 17 publications

References 110 publications

Effect of Different Culture Conditions on Anthocyanins and Related Genes in Red Pear Callus

Effect of Different Culture Conditions on Anthocyanins and Related Genes in Red Pear Callus

Influence of cytokinins and yeast extract on growth and flavone production in hairy root cultures of Scutellaria baicalensis

Production of Phenolic Compounds and Antioxidant Activity in Hairy Root Cultures of Salvia plebeia

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