Metabolic engineering of Escherichia coli for the production of 5-aminovalerate and glutarate as C5 platform chemicals

Park, Si Jae; Kim, Eun Young; Noh, Won; Park, Hye Min; Oh, Young Hoon; Lee, Seung Hwan; Song, Bong Keun; Jegal, Jonggeon; Lee, Sang Yup

doi:10.1016/j.ymben.2012.11.011

Cited by 138 publications

(140 citation statements)

References 32 publications

Supporting

Mentioning

135

Contrasting

Unclassified

Order By: Relevance

“…Recombinant E. coli WL3110 strain expressing P. putida davAB genes produced 3.6 g/L 5AVA from 7 g/L L-lysine [1]. This modified E. coli strain, however, failed to produce both L-lysine and 5AVA from glucose as the sole carbon source.…”

mentioning

confidence: 98%

“…Recently, we developed metabolic pathways for 5AVA synthesis in recombinant E. coli strains by introducing the aminovalerate pathway of P. putida [1]. Recombinant E. coli WL3110 strain expressing P. putida davAB genes produced 3.6 g/L 5AVA from 7 g/L L-lysine [1].…”

mentioning

confidence: 99%

“…This modified E. coli strain, however, failed to produce both L-lysine and 5AVA from glucose as the sole carbon source. Therefore, another E. coli strain, E. coli XQ56, which synthesizes L-lysine from glucose more effectively was engineered identically to E. coli W3110 for 5AVA production [1]. The engineered XQ56 strain produced 0.27 g/L and 0.5 g/L 5AVA from glucose in batch and fed-batch fermentation, respectively [1].…”

mentioning

confidence: 99%

“…Therefore, another E. coli strain, E. coli XQ56, which synthesizes L-lysine from glucose more effectively was engineered identically to E. coli W3110 for 5AVA production [1]. The engineered XQ56 strain produced 0.27 g/L and 0.5 g/L 5AVA from glucose in batch and fed-batch fermentation, respectively [1]. Using similar strategies, other groups have engineered recombinant E. coli to synthesize L-lysine from glucose, which is then converted to 5AVA and glutarate [2].…”

mentioning

confidence: 99%

See 3 more Smart Citations

High‐level conversion of L‐lysine into 5‐aminovalerate that can be used for nylon 6,5 synthesis

Park

Noh

et al. 2014

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

L-Lysine is a potential feedstock for the production of bio-based precursors for engineering plastics. In this study, we developed a microbial process for high-level conversion of L-lysine into 5-aminovalerate (5AVA) that can be used as a monomer in nylon 6,5 synthesis. Recombinant Escherichia coli WL3110 strain expressing Pseudomonas putida delta-aminovaleramidase (DavA) and lysine 2-monooxygenase (DavB) was grown to high density in fed-batch culture and used as a whole cell catalyst. High-density E. coli WL3110 expressing DavAB, grown to an optical density at 600 nm (OD600 ) of 30, yielded 36.51 g/L 5AVA from 60 g/L L-lysine in 24 h. Doubling the cell density of E. coli WL3110 improved the conversion yield to 47.96 g/L 5AVA from 60 g/L of L-lysine in 24 h. 5AVA production was further improved by doubling the L-lysine concentration from 60 to 120 g/L. The highest 5AVA titer (90.59 g/L; molar yield 0.942) was obtained from 120 g/L L-lysine by E. coli WL3110 cells grown to OD600 of 60. Finally, nylon 6,5 was synthesized by bulk polymerization of ϵ-caprolactam and δ-valerolactam prepared from microbially synthesized 5AVA. The hybrid system demonstrated here has promising possibilities for application in the development of industrial bio-nylon production processes.

show abstract

mentioning

confidence: 98%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

High‐level conversion of L‐lysine into 5‐aminovalerate that can be used for nylon 6,5 synthesis

Park

Noh

et al. 2014

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…To ensure the continued vitality of the polymer enterprise, it is necessary to invent high-performance polymers that are both sustainable and cost-competitive. In recent years, ingenious advances in synthetic biology have enabled the economical production of fuels (2-7), chemicals (8)(9)(10)(11)(12), and complex natural products (13-16) from renewable sugars. This elegant manipulation of existing organisms to efficiently produce valuable metabolites from inexpensive feedstocks represents a triumph of modern science and engineering and offers society the promise of renewable, environmentally compatible next-generation materials.…”

mentioning

confidence: 99%

Scalable production of mechanically tunable block polymers from sugar

Xiong¹,

Schneiderman

Bates³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

171

205

View full text Add to dashboard Cite

Significance In recent years there has been extensive research toward the development of sustainable polymeric materials. However, environmentally benign, bioderived polymers still represent a woefully small fraction of plastics and elastomers on the market today. To displace the widely useful oil-based polymers that currently dominate the industry, a bioderived synthetic polymer must be both cost and performance competitive. In this paper we address this challenge by combining the efficient bioproduction of β-methyl-δ-valerolactone with controlled polymerization techniques to produce economically viable block polymer materials with mechanical properties akin to commercially available thermoplastics and elastomers.

show abstract

Sustainable Platform Chemicals from Biomass

Juneja

2020

Green Energy to Sustainability

View full text Add to dashboard Cite

Metabolic engineering of Escherichia coli for the production of 5-aminovalerate and glutarate as C5 platform chemicals

Cited by 138 publications

References 32 publications

High‐level conversion of L‐lysine into 5‐aminovalerate that can be used for nylon 6,5 synthesis

High‐level conversion of L‐lysine into 5‐aminovalerate that can be used for nylon 6,5 synthesis

Scalable production of mechanically tunable block polymers from sugar

Sustainable Platform Chemicals from Biomass

Contact Info

Product

Resources

About