Metabolic Engineering of Escherichia coli: Increase of NADH Availability by Overexpressing an NAD+-Dependent Formate Dehydrogenase

Berrı́os-Rivera, Susana J.

doi:10.1006/mben.2002.0227

Cited by 254 publications

(170 citation statements)

References 26 publications

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“…First, we overexpressed yeast NAD + -dependent formate dehydrogenase, encoded by the fdh1 gene. The native E. coli formate dehydrogenase converts formate to CO 2 and H 2 without generation of any NADH and the yeast NAD + -dependent FDH1 generates 1 mol of NADH from 1 mol of formate (21). Second, the Bcd enzyme was replaced with the Ter enzyme that directly uses NADH as the electron donor, which was shown to be more efficient (15,22).…”

Section: Resultsmentioning

confidence: 99%

Controlled biosynthesis of odd-chain fuels and chemicals via engineered modular metabolic pathways

Tseng¹,

Prather

2012

Proc. Natl. Acad. Sci. U.S.A.

105

110

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Microbial systems are being increasingly developed as production hosts for a wide variety of chemical compounds. Broader adoption of microbial synthesis is hampered by a limited number of high-yielding natural pathways for molecules with the desired physical properties, as well as the difficulty in functionally assembling complex biosynthetic pathways in heterologous hosts. Here, we address both of these challenges by reporting the adaptation of the butanol biosynthetic pathway for the synthesis of odd-chain molecules and the development of a complementary modular toolkit to facilitate pathway construction, characterization, and optimization in engineered Escherichia coli. The modular feature of our pathway enables multientry and multiexit biosynthesis of various odd-chain compounds at high efficiency. By varying combinations of the pathway and toolkit enzymes, we demonstrate controlled production of propionate, trans-2-pentenoate, valerate, and pentanol, compounds with applications that include biofuels, antibiotics, biopolymers, and aroma chemicals. Importantly, and in contrast to a previously used method to identify limitations in heterologous amorphadiene production, our bypass strategy was effective even without the presence of freely membrane-diffusible substrates. This approach should prove useful for optimization of other pathways that use CoA-derivatized intermediates, including fatty acid β-oxidation and the mevalonate pathway for isoprenoid synthesis.biochemicals | metabolic engineering | synthetic biology

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Section: Resultsmentioning

confidence: 99%

Controlled biosynthesis of odd-chain fuels and chemicals via engineered modular metabolic pathways

Tseng¹,

Prather

2012

Proc. Natl. Acad. Sci. U.S.A.

105

110

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show abstract

“…The results were depending on whether the formate dehydrogenase was endogenous or not (originating from Candida boidinii). Adding formate to the strain with the cell-own formate dehydrogenase under aerobic conditions resulted in a large increase in the acetate production compared to the strain with the heterologous enzyme, were a small increase of the acetate production was observed [75].…”

Section: Nadh+h + /Nad +mentioning

confidence: 94%

“…It is conceivable that in coenzyme-dependent production systems, coenzyme availability and the proportion of coenzyme in the active form may play an important role in dictating the overall process yield. Hence, the manipulation of these coenzyme levels may be crucial in order to further increase production [75][76][77][78].…”

Section: Alterations In the Coenzyme Pools 374mentioning

confidence: 99%

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Minimizing acetate formation in E. coli fermentations

Mey

Maeseneire

Soetaert

et al. 2007

J Ind Microbiol Biotechnol

202

144

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Escherichia coli remains the best established production organisms in industrial biotechnology. However, during aerobic fermentation runs at high growth rates, considerable amounts of acetate are accumulated as by-product. This by-product has negative effects on growth and protein production. Over the last 20 years, substantial research efforts have been spent to reduce acetate accumulation during aerobic growth of E. coli on glucose. From the onset it was clear that this quest should not be a simple nor uncomplicated one. Simple deletion of the acetate pathway, reduced the acetate accumulation, but instead other by-products were formed. This minireview gives a clear outline of these research efforts and the outcome of them, including bioprocess level approaches and genetic approaches. Recently, the latter seems to have some promising results

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“…At the same time, endogenous pathways can be shut down or reduced [80] to optimize the balance between heterologous and endogenous biochemical activities [79]. Side effects or roadblocks encountered can be ameliorated or removed by multiple rounds of engineering [79,81,82].…”

Section: / Metabolic Engineeringmentioning

confidence: 99%

ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts

Nie

Chaillet

Becke

et al. 2016

Advances in Experimental Medicine and Biology

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Metabolic Engineering of Escherichia coli: Increase of NADH Availability by Overexpressing an NAD+-Dependent Formate Dehydrogenase

Cited by 254 publications

References 26 publications

Controlled biosynthesis of odd-chain fuels and chemicals via engineered modular metabolic pathways

Controlled biosynthesis of odd-chain fuels and chemicals via engineered modular metabolic pathways

Minimizing acetate formation in E. coli fermentations

ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts

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