Metabolic engineering of Escherichia coli for 1-butanol production

Atsumi, Shota; Cann, Anthony F.; Connor, Michael R.; Shen, Claire R.; Smith, Kevin M.; Brynildsen, Mark P.; Chou, Katherine; Hanai, Taizo; Liao, James C.

doi:10.1016/j.ymben.2007.08.003

Cited by 759 publications

(490 citation statements)

References 21 publications

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“…However, finding a sequence with specific desired properties can be difficult, particularly when only a few members of a protein family have been characterized and a detailed understanding of the structure-function relationship is lacking. The ketol-acid reductoisomerase (KARI, EC 1.1.1.86, also known as acetohydroxyacid isomeroreductase (AHAIR)) enzymes have attracted much interest for production of amino acids and biofuels (Atsumi et al, 2008a;Bastian et al, 2011;Brinkmann-Chen et al, 2013;Hasegawa et al, 2012;Liu et al, 2010). These oxidoreductases catalyze the second step in the branched chain amino-acid (BCAA) biosynthesis pathway (Chunduru et al, 1989), conversion of (S)-2-acetolactate (S2AL) to (R)-2,3-dihydroxyisovalerate (RDHIV) via a methyl shift coupled to a reduction with concomitant oxidation of a nicotinamide adenine dinucleotide cofactor.…”

Section: Introductionmentioning

confidence: 99%

Uncovering rare NADH-preferring ketol-acid reductoisomerases

Brinkmann‐Chen

Cahn

Arnold

2014

Metabolic Engineering

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All members of the ketol-acid reductoisomerase (KARI) enzyme family characterized to date have been shown to prefer the nicotinamide adenine dinucleotide phosphate hydride (NADPH) cofactor to nicotinamide adenine dinucleotide hydride (NADH). However, KARIs with the reversed cofactor preference are desirable for industrial applications, including anaerobic fermentation to produce branched-chain amino acids. By applying insights gained from structural and engineering studies of this enzyme family to a comprehensive multiple sequence alignment of KARIs, we identified putative NADHutilizing KARIs and characterized eight whose catalytic efficiencies using NADH were equal to or greater than NADPH. These are the first naturally NADH-preferring KARIs reported and demonstrate that this property has evolved independently multiple times, using strategies unlike those used previously in the laboratory to engineer a KARI cofactor switch.

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Section: Introductionmentioning

confidence: 99%

Uncovering rare NADH-preferring ketol-acid reductoisomerases

Brinkmann‐Chen

Cahn

Arnold

2014

Metabolic Engineering

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“…Although native Clostridium produces butanol and isopropanol, the fact that it is a strict anaerobe with a slow growth rate means that it is necessary to tightly control fermentation conditions in order to maximize solvent production [8]. Further, the limited genetic tools available in Clostridium currently hinder the engineering of alcohol production in this organism to reach higher yields [8,9].…”

Section: Heterologous Expression Of the Clostridialmentioning

confidence: 99%

“…Further, the limited genetic tools available in Clostridium currently hinder the engineering of alcohol production in this organism to reach higher yields [8,9]. To overcome these problems and reach higher alcohol titers, the Clostridium isopropanol pathway has been heterologously expressed in E. coli, while the butanol pathway has been expressed in both E. coli and S. cerevisiae.…”

Section: Heterologous Expression Of the Clostridialmentioning

confidence: 99%

Advanced biofuel production in microbes

Peralta‐Yahya

Keasling

2010

Biotechnology Journal

349

226

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The cost-effective production of biofuels from renewable materials will begin to address energy security and climate change concerns. Ethanol, naturally produced by microorganisms, is currently the major biofuel in the transportation sector. However, its low energy content and incompatibility with existing fuel distribution and storage infrastructure limits its economic use in the future. Advanced biofuels, such as long chain alcohols and isoprenoid-and fatty acid-based biofuels, have physical properties that more closely resemble petroleum-derived fuels, and as such are an attractive alternative for the future supplementation or replacement of petroleum-derived fuels. Here, we review recent developments in the engineering of metabolic pathways for the production of known and potential advanced biofuels by microorganisms. We concentrate on the metabolic engineering of genetically tractable organisms such as Escherichia coli and Saccharomyces cerevisiae for the production of these advanced biofuels.

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“…For example, the genes involved in the synthesis of isopropanol [ 16,17] and butanol [ 18] from -) ! Clostriduim were recently expressed in E. coli.…”

Section: Production Hostmentioning

confidence: 99%

Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

Lee

Chou

Ham

et al. 2008

Current Opinion in Biotechnology

554

302

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The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

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Metabolic engineering of Escherichia coli for 1-butanol production

Cited by 759 publications

References 21 publications

Uncovering rare NADH-preferring ketol-acid reductoisomerases

Uncovering rare NADH-preferring ketol-acid reductoisomerases

Advanced biofuel production in microbes

Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

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