Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose

Chu, Haipei; Xin, Bo; Liu, Peihai; Wang, Yu; Li, Lixiang; Liu, Xiuxiu; Zhang, Xuan; Ma, Cuiqing; Xu, Ping; Gao, Chao

doi:10.1186/s13068-015-0324-x

Cited by 29 publications

(15 citation statements)

References 40 publications

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“…One method to generate S,S-2,3-butanediol is by employing E. coli as a biocatalyst to recycle meso-2,3-butanediol and convert it to S,S-2,3butanediol (J. et al, 2010). Alternatively, the 2,3-butanediol pathway can be reengineered without acetolactate decarboxylase, thereby obtaining in vivo diacetyl biosynthesis through non-enzymatic decarboxylation of acetolactate (Chu et al, 2015). The same study has shown that addition of Fe 3+ aids decarboxylation and achieved enantiomerically pure S,S-2,3-butanediol production up to 2.2 g/L.…”

Section: Diolsmentioning

confidence: 99%

Escherichia coli as a host for metabolic engineering

Pontrelli

Chiu

Lan

et al. 2018

Metabolic Engineering

289

140

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Over the past century, Escherichia coli has become one of the best studied organisms on earth. Features such as genetic tractability, favorable growth conditions, well characterized biochemistry and physiology, and availability of versatile genetic manipulation tools make E. coli an ideal platform host for development of industrially viable productions. In this review, we discuss the physiological attributes of E. coli that are most relevant for metabolic engineering, as well as emerging techniques that enable efficient phenotype construction. Further, we summarize the large number of native and non-native products that have been synthesized by E. coli, and address some of the future challenges in broadening substrate range and fighting phage infection.

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Section: Diolsmentioning

confidence: 99%

Escherichia coli as a host for metabolic engineering

Pontrelli

Chiu

Lan

et al. 2018

Metabolic Engineering

289

140

View full text Add to dashboard Cite

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“…Therefore, the other two important genes, budB and budC, responsible for 2,3-BD production should be inserted into the glycerol metabolism pathway of E. coli BL21(DE3)pLysS/dhaD stain to get a high yield of 2,3-BD. Although there is no report on the pathway of 2,3-BD production in E. coli, several works have been done on systematic metabolic engineering of E. coli for high product yield of 2,3-BD by introducing budB and budC genes (Xu et al 2014;Chu et al 2015).…”

Section: Batch Fermentation By the Recombinant E Coli Bl21(de3) Plysmentioning

confidence: 99%

Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol

Rahman

Qin

2018

Bioresour. Bioprocess.

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Background:The thriving of biodiesel industry has led to produce 10% (v/v) crude glycerol, thus creating an overflow problem. Biofuel production is restricted by Escherichia coli due to its toxicity to bacterial cells. Therefore, a platform chemical and fuel additive 2,3-butanediol (2,3-BD) with low toxicity to microbes could be a promising alternative for biofuel production by recombinant E. coli using glycerol as the sole substrate.Results: A novel expression system of E. coli was developed to express the dhaD gene encoding glycerol dehydrogenase (GDH) to produce value-added metabolic products through aerobic biotransformation of glycerol. The dhaD gene obtained from Klebsiella pneumoniae SRP2 was expressed in E. coli BL21(DE3)pLysS using an E. coli-K. pneumoniae shuttle vector pJET1.2/blunt consisting of chloramphenicol-resistance gene under the control of the T7lac promotor. RT-PCR analysis and dhaD overexpression confirmed that the 2,3-BD synthesis pathway gene was expressed on RNA and protein levels. Therefore, the recombinant E. coli exhibited a 38.9-fold higher enzyme activity (312.57 units/ mg protein), yielding 8.97 g/L 2,3-BD, a 2.4-fold increase with respect to the non-recombinant strain. Conclusions:The engineered strain E. coli BL21(DE3)pLysS/pJET1.2/blunt-dhaD, carrying the 2,3-BD pathway gene dhaD from our newly isolated Klebsiella pneumoniae SRP2 strain, displayed the best ability to synthesize 2,3-BD from low-cost biomass glycerol. The value of expression of an important glycerol metabolism gene dhaD is the highest ever achieved with an engineered E. coli strain. From these results, the first reported dhaD expression system has paved the way for improvement of 2,3-BD production and is efficient for another heterologous gene expression in E. coli.

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“…Both myo-inositol and butane- 2,3- diol, products of glucose metabolism demonstrated significant change in abundance due to ZIKV infection ( Figure 2I ). Butane- 2,3 – diol, a volatile compound associated with certain strains of root-associated bacteria ( Kong et al, 2018 ) and capable of being produced from glucose ( Chu et al, 2015 ) was highly abundant ( P = 0.013; Log 2 5 fold change) in the infected samples ( Figure 2A ).…”

Section: Resultsmentioning

confidence: 99%

Zika Virus Infection Results in Biochemical Changes Associated With RNA Editing, Inflammatory and Antiviral Responses in Aedes albopictus

et al. 2020

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Rapid and significant range expansion of both the Zika virus (ZIKV) and its Aedes vector species has resulted in the declaration of ZIKV as a global health threat. Successful transmission of ZIKV by its vector requires a complex series of interactions between these entities including the establishment, replication and dissemination of the virus within the mosquito. The metabolic conditions within the mosquito tissues play a critical role in mediating the crucial processes of viral infection and replication and represent targets for prevention of virus transmission. In this study, we carried out a comprehensive metabolomic phenotyping of ZIKV infected and uninfected Ae. albopictus by untargeted analysis of primary metabolites, lipids and biogenic amines. We performed a comparative metabolomic study of infection state with the aim of understanding the biochemical changes resulting from the interaction between the ZIKV and its vector. We have demonstrated that ZIKV infection results in changes to the cellular metabolic environment including a significant enrichment of inosine and pseudo-uridine (Ψ) levels which may be associated with RNA editing activity. In addition, infected mosquitoes demonstrate a hypoglycemic phenotype and show significant increases in the abundance of metabolites such as prostaglandin H2, leukotriene D4 and protoporphyrinogen IX which are associated with antiviral activity. These provide a basis for understanding the biochemical response to ZIKV infection and pathology in the vector. Future mechanistic studies targeting these ZIKV infection responsive metabolites and their associated biosynthetic pathways can provide inroads to identification of mosquito antiviral responses with infection blocking potential.

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Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose

Cited by 29 publications

References 40 publications

Escherichia coli as a host for metabolic engineering

Escherichia coli as a host for metabolic engineering

Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol

Zika Virus Infection Results in Biochemical Changes Associated With RNA Editing, Inflammatory and Antiviral Responses in Aedes albopictus

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