Metabolic engineering of Escherichia coli for production of biodiesel from fatty alcohols and acetyl-CoA

Guo, Daoyi; Pan, Hong; Li, Xun

doi:10.1007/s00253-015-6809-5

Cited by 24 publications

(22 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2, 5), suggesting that the alcohol acetyltransferase encoded by ATF1 is the only endogenous enzyme capable of acetylating longer-chain alcohols to any extent. Previous work has shown that ATF1 is capable of acetylating saturated alcohols up to C14 and C16 [24]. Here, by providing exogenous alcohols not typically produced by yeast, we demonstrate that overexpressed ATF1 can acetylate an even wider range of molecules, including fatty alcohols containing up to 18 carbons, with high efficiency.…”

Section: Discussionmentioning

confidence: 57%

“…Deletion or overexpression of these genes confirmed their roles in the formation of a broad range of short-chain acetates from propyl acetate up to octyl acetate [22]. It has recently been shown that ATF1 can also acetylate saturated fatty alcohols with a chain length of 14C and 16C when expressed in Escherichia coli [23, 24]. Here, we use both in vivo and in vitro assays to compare the efficiency of the acetyltransferases for acetylation of various fatty alcohols.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

The Yeast ATF1 Acetyltransferase Efficiently Acetylates Insect Pheromone Alcohols: Implications for the Biological Production of Moth Pheromones

Ding

Lager

Bansal

et al. 2016

Lipids

View full text Add to dashboard Cite

Many moth pheromones are composed of mixtures of acetates of long-chain (≥10 carbon) fatty alcohols. Moth pheromone precursors such as fatty acids and fatty alcohols can be produced in yeast by the heterologous expression of genes involved in insect pheromone production. Acetyltransferases that subsequently catalyze the formation of acetates by transfer of the acetate unit from acetyl-CoA to a fatty alcohol have been postulated in pheromone biosynthesis. However, so far no fatty alcohol acetyltransferases responsible for the production of straight chain alkyl acetate pheromone components in insects have been identified. In search for a non-insect acetyltransferase alternative, we expressed a plant-derived diacylglycerol acetyltransferase (EaDAcT) (EC 2.3.1.20) cloned from the seed of the burning bush (Euonymus alatus) in a yeast system. EaDAcT transformed various fatty alcohol insect pheromone precursors into acetates but we also found high background acetylation activities. Only one enzyme in yeast was shown to be responsible for the majority of that background activity, the acetyltransferase ATF1 (EC 2.3.1.84). We further investigated the usefulness of ATF1 for the conversion of moth pheromone alcohols into acetates in comparison with EaDAcT. Overexpression of ATF1 revealed that it was capable of acetylating these fatty alcohols with chain lengths from 10 to 18 carbons with up to 27- and 10-fold higher in vivo and in vitro efficiency, respectively, compared to EaDAcT. The ATF1 enzyme thus has the potential to serve as the missing enzyme in the reconstruction of the biosynthetic pathway of insect acetate pheromones from precursor fatty acids in yeast.

show abstract

Section: Discussionmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 98%

The Yeast ATF1 Acetyltransferase Efficiently Acetylates Insect Pheromone Alcohols: Implications for the Biological Production of Moth Pheromones

Ding

Lager

Bansal

et al. 2016

Lipids

View full text Add to dashboard Cite

show abstract

“…Overnight cultures inoculated from single colonies were used to inoculate shake flasks containing M9 medium with 20 g/L glucose as previously described by Guo et al (2015), and shaken at 30 °C as above. The cells were induced at OD600 0.6–0.8 with 0.1 mM IPTG.…”

Section: Methodsmentioning

confidence: 99%

“…Cell cultures were harvested and prepared for wax esters using a previously published method (Guo et al 2015). GC/MS analysis was performed with a DB-5 capillary column.…”

Section: Methodsmentioning

confidence: 99%

Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine

et al. 2017

Self Cite

View full text Add to dashboard Cite

Phenylacetic acid (PAA) is a fine chemical with a high industrial demand for its widespread uses. Whereas, microorganic synthesis of PAA is impeded by the formation of by-product phenethyl alcohol due to quick, endogenous, and superfluous conversion of aldehydes to their corresponding alcohols, which resulted in less conversation of PAA from aldehydes. In this study, an Escherichia coli K-12 MG1655 strain with reduced aromatic aldehyde reduction (RARE) that does duty for a platform for aromatic aldehyde biosynthesis was used to prompt more PAA biosynthesis. We establish a microbial biosynthetic pathway for PAA production from the simple substrate phenylalanine in E. coli with heterologous coexpression of aminotransferase (ARO8), keto acid decarboxylase (KDC) and aldehyde dehydrogenase H (AldH) gene. It was found that PAA transformation yield was up to ~94% from phenylalanine in E. coli and there was no by-product phenethyl alcohol was detected. Our results reveal the high efficiency of the RARE strain for production of PAA and indicate the potential industrial applicability of this microbial platform for PAA biosynthesis.

show abstract

“…In this context, one alternative that has stood out is the use of biofuels, such as charcoal, alcohol, biogas and biodiesel. 1,2 Biodiesel is a renewable fuel produced either from vegetable sources (soybean, castor oil, palm oil, sunflower, among others) or from ethanol (from sugarcane) or methanol (which can be obtained from wood biomass). 3 It is, therefore, a biodegradable, sustainable and environmentally friendly fuel.…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel and Simple Electroanalytical Procedure for the Determination of Copper in Biofuel Employing a Sensor Based on Vulcan Functionalized with Carbazone

Freitas¹,

Lima²,

Marques³

et al. 2018

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

A novel and simple electroanalytical method for the determination of Cu 2+ in biodiesel samples by stripping voltammetric analysis was developed. The method employs a carbon paste electrode (CPE) modified with Vulcan carbon, previouly functionalized with carbazone (CBZ). The CPE/Vulcan-CBZ sensor promoted a significant increase in the analytical signal obtained from copper as compared to unmodified CPE, and the CPE modified with Vulcan carbon (CPE/ Vulcan). Vulcan-CBZ, Vulcan and CBZ materials were characterized by Fourier transform infrared spectroscopy (FTIR) technique. The electrochemical behavior of the sensor was evaluated using cyclic voltammetry (CV) and square-wave anodic stripping voltammetric (SWASV) techniques. The CPE/Vulcan-CBZ modified electrode showed excellent response and was able to detect Cu 2+ at nanomolar levels. The electrochemical method is based on preconcentration of Cu 2+ ions on the CPE/Vulcan-CBZ at 0.35 V vs. Ag/AgCl(sat) in 0.2 mol L -1 ammonium sulfate solution ((NH 4 ) 2 SO 4 ), pH 3.5, during 120 s, followed by subsequent chemical stripping. The analytical signal showed a linear response for Cu 2+ concentrations in the range from 6 to 190 nmol L -1 (r = 0.998), with a detection limit of 1.2 nmol L -1 . The sensor was successfully applied for the determination of Cu 2+ in biodiesel and the average recovery varied between 97.0 and 102.8% for the soybean biodiesel samples and between 109.6 and 111.0% for the babassu biodiesel samples showing a good accuracy for the proposed method.Keywords: biofuel, copper, voltammetric sensor, Vulcan carbon, carbazone IntroductionThe pursuit of alternative fuels has been acquiring prominence in the last decades. The replacement of fossil fuels has been motivated by environmental, economical and social factors, since society itself depends on its use. In this context, one alternative that has stood out is the use of biofuels, such as charcoal, alcohol, biogas and biodiesel. 1,2 Biodiesel is a renewable fuel produced either from vegetable sources (soybean, castor oil, palm oil, sunflower, among others) or from ethanol (from sugarcane) or methanol (which can be obtained from wood biomass). 3 It is, therefore, a biodegradable, sustainable and environmentally friendly fuel. However, some metals, such as Cu, Fe, and Ni, can be inserted in the production process with the catalysts, during transportation and storage. Furthermore, the presence of these metals, even at trace levels, can promote the formation of gums and sediments that damage vehicle engines and promote the oxidation of biodiesel. 4,5 Copper, iron and the effect of nickel on biodiesel's stability has been widely evaluated. Among these metals, copper is the most powerful catalyst in biodiesel's oxidation process. 5 Therefore, the monitoring of the amount of copper is extremely important in this fuel for preventing undesirable variations in its physical state and properties, during its use or storage. 5 In this context, it is opportune and necessary to develop novel sensitive and selective anal...

show abstract

Metabolic engineering of Escherichia coli for production of biodiesel from fatty alcohols and acetyl-CoA

Cited by 24 publications

References 54 publications

The Yeast ATF1 Acetyltransferase Efficiently Acetylates Insect Pheromone Alcohols: Implications for the Biological Production of Moth Pheromones

The Yeast ATF1 Acetyltransferase Efficiently Acetylates Insect Pheromone Alcohols: Implications for the Biological Production of Moth Pheromones

Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine

Development of a Novel and Simple Electroanalytical Procedure for the Determination of Copper in Biofuel Employing a Sensor Based on Vulcan Functionalized with Carbazone

Contact Info

Product

Resources

About