Metabolic engineering of fatty alcohol production in transgenic hairy roots of Crambe abyssinica

Abstract

Biotechnological production of fatty alcohols, important raw materials in the chemical industry, has been receiving considerable attention in recent years. Fatty alcohols are formed by the reduction of fatty acyl-CoAs or fatty acyl-ACPs catalyzed by a fatty acyl reductase (FAR). In this study, we introduced genes encoding FARs from Arabidopsis thaliana (AtFAR5) and Simmondsia chinensis (ScFAR) into Crambe abyssinica hairy roots via Agrobacterium rhizogenes-mediated transformation. The efficiency of the transformation ranged between 30 and 45%. The fatty alcohols were only detected in the transgenic hairy root lines expressing ScFAR gene. In all tested lines stearyl alcohol (18:0-OH), arachidyl alcohol (20:0-OH), and behenyl alcohol (22:0-OH) were produced. The content of 18:0-OH varied from 1 to 3% of total fatty acids and fatty alcohols, while the amount of either 20:0-OH and 22:0-OH did not exceed 2%. The transgenic hairy root lines produced from 0.98 to 2.59 nmol of fatty alcohols per mg of dry weight. Very low activity of ScFAR was detected in the microsomal fractions isolated from the selected hairy root lines. To our knowledge, this is the first report on the fatty alcohol production in the hairy root cultures. Biotechnol. Bioeng. 2017;114: 1275-1282. © 2016 Wiley Periodicals, Inc.

Comparative Investigation of Combined Metabolomics-Flavoromics during the Ripening of Mango (Mangifera indica L.) cv. ‘Nam Dok Mai Si Thong’ and ‘Nam Dok Mai No. 4’

“…Comparatively, the total lipid classes at 0 days were one-fold higher than those detected at 8 days of ripening in both cultivars and seasons. These fatty alcohols might be formed by reducing fatty acyl-CoAs or fatty acyl-ACPs catalyzed by a fatty acyl reductase [ 16 ]. Rowland et al reported that NADPH-dependent fatty acyl reductases catalyze the development of fatty alcohols from fatty acyl-CoAs or fatty acyl-ACPs [ 17 ].…”

An Extract of Transgenic Senna obtusifolia L. hairy roots with Overexpression of PgSS1 Gene in Combination with Chemotherapeutic Agent Induces Apoptosis in the Leukemia Cell Line

“…Transgenic plants are increasingly becoming an extremely attractive source of desirable biologically-active compounds [25][26][27][28][29][30][31]. The dynamic development of genetic engineering tools now allows plants to be modified for the most efficient production of many therapeutics, such as recombinant proteins or secondary metabolites.…”

New insights into diosgenin biosynthesis pathway and its regulation in Trigonella foenum‐graecum L.

“…Considering these, the aim of this research was to study the metabolic pathway and biosynthetic genes functioning in diosgenin formation. The results could be applied to engineer the metabolic flux toward diosgenin biosynthesis in order to improve the accumulation of diosgenin in either the whole plant or the hairy root cultures of fenugreek, which have been considered as promising alternative platforms as compared to the wild plant . In the present study, the full‐length CDS of 14 genes involved in the diosgenin biosynthesis pathway were identified (Figures S5B–S15C).…”