Metabolic engineering of Klebsiella pneumoniae J2B for co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol: Reduction of acetate and other by-products

Ko, Yeounjoo; Seol, Eunhee; Sekar, Balaji Sundara; Kwon, Seongjin; Lee, Jaehyeon; Park, Sunghoon

doi:10.1016/j.biortech.2017.08.099

Cited by 44 publications

(38 citation statements)

References 40 publications

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“…However, there are other microorganisms that could be suitable and efficient cell factories for efficient production of 3HP. For example, Lactobacillus reuteri is a good candidate, due to its ability to naturally produce the coenzyme B 12 and to its high acid tolerance-both of which are required when producing 3HP. Moreover, the pdu operon is endogenous in that specific species [40], minimizing the need for gene transfer.…”

Section: Other Microorganismsmentioning

confidence: 99%

“…Formate production initially increased with increasing levels of aeriation, followed by a sharp decrease when aeration was higher than 0.6 vvm. Ko et al [12] also attempted to control the production of other metabolic by-products, especially of acetic acid, with the aim of improving co-production of 3HP and 1,3-propanediol from the recombinant strain of K. pneumoniae (J2B), which overexpressed the ald gene (encoding alpha-ketoglutaric semialdehyde dehydrogenase-kgsadh). The methods that they evaluated for acetate reduction were reduction of the glycerol assimilation through the glycolytic pathway, increasing the glycerol flow towards 3HP and 1,3-propanediol formation, and finally controlling the aeration levels.…”

Section: Engineering Of K Pneumoniae Cells For the Conversion Of Glymentioning

confidence: 99%

“…Regeneration of NAD + is important, as it is required for the action of the ald enzyme. NAD + can be regenerated in the electron transport chain, which requires increased aeration, but the presence of oxygen inhibits the synthesis of coenzyme B12, which is also involved in the process [12]. Moreover, as will be discussed later, oxygen also inactivates the glycerol dehydratase.…”

Section: Introductionmentioning

confidence: 99%

“…Regeneration of NAD + can also take place during the conversion of 3-hydroxypropionaldehyde to 1,3-propanediol by the action of the 1,3-propanediol oxidoreductase, which consumes NADH [13]. If the 1,3-propanediol oxidoreductase gene is knocked out, another pathway for NAD + should be found in order to maintain the balance between NADH and NAD + , such as lactate or acetate formation [12]. On the other hand, co-production of 3HP and 1,3-propanediol can also be desirable, as 1,3-propanediol also has significant applications and these two molecules can be separated easily, due to the presence of different functional groups on the molecules [14].…”

Section: Introductionmentioning

confidence: 99%

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Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

et al. 2018

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Abstract:The production of high added-value chemicals from renewable resources is a necessity in our attempts to switch to a more sustainable society. 3-Hydroxypropionic acid (3HP) is a promising molecule that can be used for the production of an important array of high added-value chemicals, such as 1,3-propanediol, acrylic acid, acrylamide, and bioplastics. Biological production of 3HP has been studied extensively, mainly from glycerol and glucose, which are both renewable resources. To enable conversion of these carbon sources to 3HP, extensive work has been performed to identify appropriate biochemical pathways and the enzymes that are involved in them. Novel enzymes have also been identified and expressed in host microorganisms to improve the production yields of 3HP. Various process configurations have also been proposed, resulting in improved conversion yields. The intense research efforts have resulted in the production of as much as 83.8 g/L 3HP from renewable carbon resources, and a system whereby 3-hydroxypropionitrile was converted to 3HP through whole-cell catalysis which resulted in 184.7 g/L 3HP. Although there are still challenges and difficulties that need to be addressed, the research results from the past four years have been an important step towards biological production of 3HP at the industrial level.

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Section: Other Microorganismsmentioning

confidence: 99%

Section: Engineering Of K Pneumoniae Cells For the Conversion Of Glymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

et al. 2018

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“…Deletion of ldhA dramatically boosted 3-HP production, at the same time, 1,3-propanediol production was also enhanced to 18.5 g/L. Ko et al (2017) reported that 43 g/L of 3-HP and 21 g/L of 1,3-propanediol were obtained by reducing acetate and other by-products. Although 1,3-propanediol formation benefits glycerol utilization by regenerating cofactor, it accounts for a substantial portion of glycerol carbon flux.…”

Section: Effect Of Dhat Deficiency On 3-hp Productionmentioning

confidence: 97%

Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae

Jiang

Huang

et al. 2018

Bioresour. Bioprocess.

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Background: 3-Hydroxypropionic acid (3-HP) is one of the top value-added chemicals that can be produced from renewable resources. Crude glycerol, a cheap feedstock, is the foremost by-product generated from biodiesel industries. In this study, Klebsiella pneumonia was engineered for efficient 3-HP production from glycerol.Results: Three metabolically engineered K. pneumoniae strains were constructed and the strain JJQ02 (ΔldhAΔdhaT) overexpressing aldehyde dehydrogenase from E. coli was the most suitable for 3-HP production. This strain produced 61.9 g/L of 3-HP (a yield of 0.58 mol/mol) in 38 h in a 5-L fermentor by control of the aeration rate. Deletion of ldhA and dhaT effectively eliminated lactate formation and reduced 1,3-propanediol production, but more 2,3-butanediol was accumulated due to redox balance. The fed-batch process was successfully scaled-up in a 300-L bioreactor where the 3-HP production level was 54.5 g/L (a yield of 0.43 mol/mol) in 51 h. Conclusions:Deficiency of both lactate dehydrogenase and 1,3-propanediol oxidoreductase as well as control of aeration significantly enhanced 3-HP production, and the successful scale-up of the fed-batch process in a 300-L bioreactor indicated a great potential to industrial production of 3-HP. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Co‐production of microbial lipids with valuable chemicals

Lin

Qian

Zhang

et al. 2021

Biofuels Bioprod Bioref

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Microbial lipids are promising substitutes for petroleum and plant oil. However, the industrialization of microbial lipids is still limited and cannot compete with petrochemical petroleum due to its high production cost. Microorganisms capable of accumulating lipids and additional valuable chemicals are attractive for improving the economics of the microbial lipid production process. Compared with the increase in production of complex metabolic engineering to improve the overall economy, the multi‐chemical co‐production system is more advantageous in balancing the metabolic flux and optimal cell physiological recovery. In this review, various chemicals co‐produced with microbial lipids are classified according to their cell location. Efficient strategies, including the fermentation process and genetic engineering for regulating and enhancing such co‐production systems, are comprehensively discussed. The prospective and challenges during the scale‐up of lipids and valuable chemicals co‐production process are also highlighted. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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Metabolic engineering of Klebsiella pneumoniae J2B for co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol: Reduction of acetate and other by-products

Cited by 44 publications

References 40 publications

Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

Biological Production of 3-Hydroxypropionic Acid: An Update on the Current Status

Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae

Co‐production of microbial lipids with valuable chemicals

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