Metabolic engineering of type II methanotroph, Methylosinus trichosporium OB3b, for production of 3-hydroxypropionic acid from methane via a malonyl-CoA reductase-dependent pathway

Nguyen, Diep Thi Ngoc; Lee, Ok Kyung; Lim, Chaeil; Lee, Jinwon; Na, Jeong-Geol; Lee, Eun Yeol

doi:10.1016/j.ymben.2020.02.002

Cited by 42 publications

(10 citation statements)

References 54 publications

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“…More recently, a type II methanotroph, Methylosinus trichosporium OB3b, was first metabolically modified to produce 3‐HP from methane through a MCR‐dependent pathway. Although a fairly low titer of 60.59 mg/L 3‐HP was obtained in 42 h utilizing the engineered strain via bioreactor operation, it opened the door for utilizing type II methanotrophs as cell factories for biochemical production accompanied by reduction of greenhouse gases (Nguyen et al, 2020).…”

Section: Producing 3‐hp From Other Carbon Sourcesmentioning

confidence: 99%

Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid

Liang

Sun

Nie

et al. 2022

Biotech & Bioengineering

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As an attractive and valuable platform chemical, 3‐hydroxypropionic acid (3‐HP) can be used to produce a variety of industrially important commodity chemicals and biodegradable polymers. Moreover, the biosynthesis of 3‐HP has drawn much attention in recent years due to its sustainability and environmental friendliness. Here, we focus on recent advances, challenges, and metabolic engineering strategies in the biosynthesis of 3‐HP. While glucose and glycerol are major carbon sources for its production of 3‐HP via microbial fermentation, other carbon sources have also been explored. To increase yield and titer, synthetic biology and metabolic engineering strategies have been explored, including modifying pathway enzymes, eliminating flux blockages due to byproduct synthesis, eliminating toxic byproducts, and optimizing via genome‐scale models. This review also provides insights on future directions for 3‐HP biosynthesis.

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Section: Producing 3‐hp From Other Carbon Sourcesmentioning

confidence: 99%

Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid

Liang

Sun

Nie

et al. 2022

Biotech & Bioengineering

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“…Type II methanotrophs maintain high acetyl-CoA flux, which is a particularly useful trait in deriving valuable chemicals from C1 feedstocks ( Nguyen et al, 2021 ). By engineering the malonyl-CoA pathway, methane fed cultures in nitrate mineral salt medium achieved 60.59 mg/L 3-HP ( Nguyen et al, 2020 ). An optimized titer of 69.8 mg/L 3-HP has also been demonstrated in the methylotroph M. extorquens on a supplemented minimal medium ( Yang et al, 2017 ).…”

Section: Biochemical Precursorsmentioning

confidence: 99%

Translating advances in microbial bioproduction to sustainable biotechnology

Carruthers

Lee

2022

Front. Bioeng. Biotechnol.

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Advances in synthetic biology have radically changed our ability to rewire microorganisms and significantly improved the scalable production of a vast array of drop-in biopolymers and biofuels. The success of a drop-in bioproduct is contingent on market competition with petrochemical analogues and weighted upon relative economic and environmental metrics. While the quantification of comparative trade-offs is critical for accurate process-level decision making, the translation of industrial ecology to synthetic biology is often ambiguous and assessment accuracy has proven challenging. In this review, we explore strategies for evaluating industrial biotechnology through life cycle and techno-economic assessment, then contextualize how recent developments in synthetic biology have improved process viability by expanding feedstock availability and the productivity of microbes. By juxtaposing biological and industrial constraints, we highlight major obstacles between the disparate disciplines that hinder accurate process evaluation. The convergence of these disciplines is crucial in shifting towards carbon neutrality and a circular bioeconomy.

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“…They have been reported for bioremediation, bioleaching, and epoxidation. Conjugation (Nguyen et al, 2020) and electroporation-based (Yan et al, 2016) gene-transfer techniques have been developed for methanotrophs. However, the yield and productivity from methanotrophic cell factories are relatively low as compared to other hosts such as E. coli (Lee et al, 2016).…”

Section: Methanotrophsmentioning

confidence: 99%

Biotransformation of Methane and Carbon Dioxide Into High-Value Products by Methanotrophs: Current State of Art and Future Prospects

2021

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Conventional chemical methods to transform methane and carbon dioxide into useful chemicals are plagued by the requirement for extreme operating conditions and expensive catalysts. Exploitation of microorganisms as biocatalysts is an attractive alternative to sequester these C1 compounds and convert them into value-added chemicals through their inherent metabolic pathways. Microbial biocatalysts are advantageous over chemical processes as they require mild-operating conditions and do not release any toxic by-products. Methanotrophs are potential cell-factories for synthesizing a wide range of high-value products via utilizing methane as the sole source of carbon and energy, and hence, serve as excellent candidate for methane sequestration. Besides, methanotrophs are capable of capturing carbon dioxide and enzymatically hydrogenating it into methanol, and hence qualify to be suitable candidates for carbon dioxide sequestration. However, large-scale production of value-added products from methanotrophs still presents an overwhelming challenge, due to gas-liquid mass transfer limitations, low solubility of gases in liquid medium and low titer of products. This requires design and engineering of efficient reactors for scale-up of the process. The present review offers an overview of the metabolic architecture of methanotrophs and the range of product portfolio they can offer. Special emphasis is given on methanol biosynthesis as a potential biofuel molecule, through utilization of methane and alternate pathway of carbon dioxide sequestration. In view of the gas-liquid mass transfer and low solubility of gases, the key rate-limiting step in gas fermentation, emphasis is given toward reactor design consideration essential to achieve better process performance.

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Metabolic engineering of type II methanotroph, Methylosinus trichosporium OB3b, for production of 3-hydroxypropionic acid from methane via a malonyl-CoA reductase-dependent pathway

Cited by 42 publications

References 54 publications

Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid

Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid

Translating advances in microbial bioproduction to sustainable biotechnology

Biotransformation of Methane and Carbon Dioxide Into High-Value Products by Methanotrophs: Current State of Art and Future Prospects

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