Metabolic engineering strategies to enable microbial utilization of C1 feedstocks

Jiang, Wei; Villamor, David Hernández; Peng, Huadong; Chen, Jian; Liu, Long; Haritos, Victoria S.; Ledesma-Amaro, Rodrigo

doi:10.1038/s41589-021-00836-0

Cited by 110 publications

(62 citation statements)

References 96 publications

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“…Other novel biotechnology strategies include metabolic engineering to enable microbial utilization of using CO 2 , CH 4 , and other C1 feedstocks for the production of microbial proteins rich in essential amino acids. 139 , 140 These proteins can be used as substitutes for animal proteins. Current advances in biotechnology provide a powerful platform for the production of protein-rich feed or food additives in the form of fungal, algae, yeast, and bacterial cell biomass.…”

Section: Technologies For Enhanced Carbon Sink In Global Ecosystemsmentioning

confidence: 99%

Technologies and perspectives for achieving carbon neutrality

et al. 2021

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Global development has been heavily reliant on the overexploitation of natural resources since the Industrial Revolution. With the extensive use of fossil fuels, deforestation, and other forms of land-use change, anthropogenic activities have contributed to the ever-increasing concentrations of greenhouse gases (GHGs) in the atmosphere, causing global climate change. In response to the worsening global climate change, achieving carbon neutrality by 2050 is the most pressing task on the planet. To this end, it is of utmost importance and a significant challenge to reform the current production systems to reduce GHG emissions and promote the capture of CO 2 from the atmosphere. Herein, we review innovative technologies that offer solutions achieving carbon (C) neutrality and sustainable development, including those for renewable energy production, food system transformation, waste valorization, C sink conservation, and C-negative manufacturing. The wealth of knowledge disseminated in this review could inspire the global community and drive the further development of innovative technologies to mitigate climate change and sustainably support human activities.

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Section: Technologies For Enhanced Carbon Sink In Global Ecosystemsmentioning

confidence: 99%

Technologies and perspectives for achieving carbon neutrality

et al. 2021

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“…In the past decades, several review papers have described different aspects of formaldehyde metabolism in bacteria and, among others, the following issues were covered: (i) Stress responses of bacterial pathogens to formaldehyde in the environment [19]; (ii) Different cofactor-dependent formaldehyde oxidation pathways in methylotrophic bacteria [29]; (iii) Native methanol metabolism as a foundation for the design of synthetic methylotrophy [30]; (iv) Challenges and opportunities regarding the engineering of unnatural methylotrophic cell factories for methanol-and formate-based biomanufacturing [31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

“…Altogether, we present an exhaustive description of pathways involved in formaldehyde metabolism, both in methylotrophs and nonmethylotrophs, together with examples of how this crucial knowledge has been applied in the establishment of synthetic methylotrophy thus far (Figure 1). methylotrophy [30]; (iv) Challenges and opportunities regarding the engineering of natural methylotrophic cell factories for methanol-and formate-based biomanufactu [31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy

et al. 2022

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Formaldehyde metabolism is prevalent in all organisms, where the accumulation of formaldehyde can be prevented through the activity of dissimilation pathways. Furthermore, formaldehyde assimilatory pathways play a fundamental role in many methylotrophs, which are microorganisms able to build biomass and obtain energy from single- and multicarbon compounds with no carbon–carbon bonds. Here, we describe how formaldehyde is formed in the environment, the mechanisms of its toxicity to the cells, and the cell’s strategies to circumvent it. While their importance is unquestionable for cell survival in formaldehyde rich environments, we present examples of how the modification of native formaldehyde dissimilation pathways in nonmethylotrophic bacteria can be applied to redirect carbon flux toward heterologous, synthetic formaldehyde assimilation pathways introduced into their metabolism. Attempts to engineer methylotrophy into nonmethylotrophic hosts have gained interest in the past decade, with only limited successes leading to the creation of autonomous synthetic methylotrophy. Here, we discuss how native formaldehyde assimilation pathways can additionally be employed as a premise to achieving synthetic methylotrophy. Lastly, we discuss how emerging knowledge on regulation of formaldehyde metabolism can contribute to creating synthetic regulatory circuits applied in metabolic engineering strategies.

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“…Enzymes play an essential role in the microbial carbon fixation process, which also exert considerable influence on the CO 2 fixation pathways; for example, the irreplaceable role of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) ( Bhat et al, 2017 ) and formate dehydrogenase ( Kumar et al, 2017 ) in the Calvin cycle ( Figure 1 ) and the Wood–Ljungdahl pathway, respectively. Thus, the key enzymes and metabolic pathways involved in the existing CO 2 metabolic pathways provide essential references for developing and enhancing enzymatic conversion and microbial fixation of CO 2 ( Jiang et al, 2021 ; Nisar et al, 2021 ; Tan and Ng, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Applications of Synthetic Biotechnology on Carbon Neutrality Research: A Review on Electrically Driven Microbial and Enzyme Engineering

Zhuang

Zhang

Xiao

et al. 2022

Front. Bioeng. Biotechnol.

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With the advancement of science, technology, and productivity, the rapid development of industrial production, transportation, and the exploitation of fossil fuels has gradually led to the accumulation of greenhouse gases and deterioration of global warming. Carbon neutrality is a balance between absorption and emissions achieved by minimizing carbon dioxide (CO2) emissions from human social productive activity through a series of initiatives, including energy substitution and energy efficiency improvement. Then CO2 was offset through forest carbon sequestration and captured at last. Therefore, efficiently reducing CO2 emissions and enhancing CO2 capture are a matter of great urgency. Because many species have the natural CO2 capture properties, more and more scientists focus their attention on developing the biological carbon sequestration technique and further combine with synthetic biotechnology and electricity. In this article, the advances of the synthetic biotechnology method for the most promising organisms were reviewed, such as cyanobacteria, Escherichia coli, and yeast, in which the metabolic pathways were reconstructed to enhance the efficiency of CO2 capture and product synthesis. Furthermore, the electrically driven microbial and enzyme engineering processes are also summarized, in which the critical role and principle of electricity in the process of CO2 capture are canvassed. This review provides detailed summary and analysis of CO2 capture through synthetic biotechnology, which also pave the way for implementing electrically driven combined strategies.

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Metabolic engineering strategies to enable microbial utilization of C1 feedstocks

Cited by 110 publications

References 96 publications

Technologies and perspectives for achieving carbon neutrality

Technologies and perspectives for achieving carbon neutrality

Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy

Applications of Synthetic Biotechnology on Carbon Neutrality Research: A Review on Electrically Driven Microbial and Enzyme Engineering

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