Metabolic Engineering to Develop a Pathway for the Selective Cleavage of Carbon-Nitrogen Bonds

Kilbane, John J.

doi:10.2172/887496

Cited by 1 publication

(2 citation statements)

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“…In conclusion, aside from the lac promoter and derivatives (e.g. tac or trc promoters), only a handful of others have been shown to function in Rhodococcus: tipA (Dong et al, 2004), ace , dapA (Knoppová et al, 2007), and p45 (Veselý et al, 2003;Kilbane II, 2006). Using the developed modular plasmid pSRKBB, researchers can now easily expand the library of characterized promoters available for Rhodococcus.…”

Section: Discussionmentioning

confidence: 99%

“…In a prior study, it was reported that R. erythropolis grew robustly in concentrations of kanamycin as high as 250 µg mL -1 (Kilbane II, 2006). We wondered if the population distribution was a result of inadequate selection pressure, therefore we decided to see if we could shift the distribution of the fluorescent population toward a higher percentage by increasing the kanamycin concentration.…”

Section: Figurementioning

confidence: 98%

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Construction of a BioBrick™ compatible vector system for Rhodococcus

Ellinger

Schmidt‐Dannert

2017

Plasmid

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Throughout the past decade, the field of synthetic biology has grown rapidly. By using assembly platforms such as BioBricks™, scientists can quickly and easily build gene circuits or multi-step pathways. One limitation, however, is that most of these parts were designed and characterized with Escherichia coli as the target chassis. As a consequence, there exists a lack of standardized and well characterized or BioBrick™ compatible plasmid backbones that replicate in other potential non-model chassis organisms. The Gram-positive bacteria of the genus Rhodococcus represent an interesting chassis for biotechnological applications due to their tremendous metabolic capabilities. In this report we describe our progress toward developing a BioBrick™ compatible plasmid system for Rhodococcus. We demonstrate its utility for heterologous protein expression through flow cytometric analysis of the lac promoter in the oleaginous strain Rhodococcus opacus PD630.

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