Metabolic evolution of two reducing equivalent-conserving pathways for high-yield succinate production in Escherichia coli

Zhu, Xinna; Tan, Zhongyang; Xu, Huji; Chen, Jing; Tang, Ji‐Xin; Zhang, Xueli

doi:10.1016/j.ymben.2014.05.003

Cited by 105 publications

(81 citation statements)

References 45 publications

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“…High-succinate-producing E. coli strain HX024 was previously obtained through a combination of genetic engineering and metabolic evolution (9). A mutation in cusS (G629T) was found in strain HX024 (9) that we speculated was related to osmotolerance.…”

Section: Resultsmentioning

confidence: 99%

“…In order to verify that the cusS mutation led to osmotolerance in E. coli, this mutation was introduced into parent strain Suc-T110, which was not adaptively evolved (9), and strain NZ-504 was obtained (Table 1). To compare the osmotolerance capacities of strains NZ-504 and Suc-T110, both a high initial glucose concentration (12%) and disodium succinate supplementation (30 g/liter) were used as osmotic inhibitors.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous work, high-succinate-producing E. coli strain HX024 was obtained by a combination of genetic engineering and metabolic evolution (9). Using sodium salt (2.4 M Na 2 CO 3 and 1.2 M NaOH) as the neutralizer to maintain the pH, strain HX024 produced 813 mM succinate.…”

Section: Importancementioning

confidence: 99%

“…This suggests that strain HX024 developed its osmotolerant capacity through metabolic evolution. Through genome sequencing of strain HX024, a mutation in cusS (G629T), which encodes the CusS sensory histidine kinase, was identified (9). Two-component systems (TCSs) are widely distributed in microorganisms that encounter hostile environments.…”

Section: Importancementioning

confidence: 99%

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Osmotolerance in Escherichia coli Is Improved by Activation of Copper Efflux Genes or Supplementation with Sulfur-Containing Amino Acids

Xiao

Zhu

Feng

et al. 2017

Appl Environ Microbiol

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Improvement in the osmotolerance of Escherichia coli is essential for the production of high titers of various bioproducts. In this work, a cusS mutation that was identified in the previously constructed high-succinate-producing E. coli strain HX024 was investigated for its effect on osmotolerance. CusS is part of the twocomponent system CusSR that protects cells from Ag(I) and Cu(I) toxicity. Changing cusS from strain HX024 back to its original sequence led to a 24% decrease in cell mass and succinate titer under osmotic stress (12% glucose). When cultivated with a high initial glucose concentration (12%), introduction of the cusS mutation into parental strain Suc-T110 led to a 21% increase in cell mass and a 40% increase in succinate titer. When the medium was supplemented with 30 g/liter disodium succinate, the cusS mutation led to a 120% increase in cell mass and a 492% increase in succinate titer. Introducing the cusS mutation into the wild-type strain ATCC 8739 led to increases in cell mass of 87% with 20% glucose and 36% using 30 g/liter disodium succinate. The cusS mutation increased the expression of cusCFBA, and gene expression levels were found to be positively related to osmotolerance abilities. Because high osmotic stress has been associated with deleterious accumulation of Cu(I) in the periplasm, activation of CusCFBA may alleviate this effect by transporting Cu(I) out of the cells. This hypothesis was confirmed by supplementing sulfurcontaining amino acids that can chelate Cu(I). Adding methionine or cysteine to the medium increased the osmotolerance of E. coli under anaerobic conditions. IMPORTANCE In this work, an activating Cus copper efflux system was found to increase the osmotolerance of E. coli. In addition, new osmoprotectants were identified. Supplementation with methionine or cysteine led to an increase in osmotolerance of E. coli under anaerobic conditions. These new strategies for improving osmotolerance will be useful for improving the production of chemicals in industrial bioprocesses.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Importancementioning

confidence: 99%

Section: Importancementioning

confidence: 99%

See 2 more Smart Citations

Osmotolerance in Escherichia coli Is Improved by Activation of Copper Efflux Genes or Supplementation with Sulfur-Containing Amino Acids

Xiao

Zhu

Feng

et al. 2017

Appl Environ Microbiol

Self Cite

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show abstract

“…Exogenous synthetic routes were introduced (pyruvate carboxylase) (Sanchez et al, 2005). Cofactors regulation (Liang et al, 2012) and evolution engineering (Zhang et al, 2009;Zhu et al, 2014) were also studied. Generally, glucose or xylose was used as the substrate for the engineered E. coli.…”

Section: Introductionmentioning

confidence: 99%

Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli

Bai

Zhou

Yang

et al. 2015

Bioresource Technology

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Succinic Acid

2023

Pathway Design for Industrial Fermentation

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Metabolic evolution of two reducing equivalent-conserving pathways for high-yield succinate production in Escherichia coli

Cited by 105 publications

References 45 publications

Osmotolerance in Escherichia coli Is Improved by Activation of Copper Efflux Genes or Supplementation with Sulfur-Containing Amino Acids

Osmotolerance in Escherichia coli Is Improved by Activation of Copper Efflux Genes or Supplementation with Sulfur-Containing Amino Acids

Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli

Succinic Acid

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