Metabolic Exploration of Muscle Biopsy

Charles, Anne-Laure; Dufour, Stéphane; Tran, Thuong; Bouitbir, Jamal; Gény, Bernard; Zoll, Joffrey

doi:10.5772/31588

Cited by 1 publication

(2 citation statements)

References 92 publications

(86 reference statements)

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“…Skeletal muscle can increase its ATP turnover, allowing it to transition from rest to exercise/ contractile activity, where different energy-producing substrates (high-energy phosphate compounds, glucose, glycogen, and lipids) can be oxidized (49,66,84,135). The most concentrated substrates in skeletal muscle are glycogen and phosphocreatine (PCr) (192), with higher PCr levels in glycolytic muscles (26). Some authors have demonstrated that ATP content remains stable during contraction (49,84), while others have found that it decreases (72,106); this discrepancy may be explained by the use of different species and contraction protocols.…”

Section: Mitochondria the "Powerhouse" Of The Cellsmentioning

confidence: 99%

“…During ischemia, the nutrient-and oxygen-deprived blood supply cannot meet the energy demands of muscles, leading to numerous ionic and metabolic changes. The low ATP reserve is used predominantly to maintain membrane potential and ion compartmentalization, but because of oxygen deprivation, mitochondrial OXPHOS and the electron transport chain are inhibited, and the mitochondrial membrane potential decreases (26,102). Brandão et al (17) showed in isolated rat mitochondria that respiration and membrane potential are altered after 5 h of ischemia.…”

Section: Chronology Of Events At the Level Of Skeletal Muscle Fiber Cmentioning

confidence: 99%

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Chronology of mitochondrial and cellular events during skeletal muscle ischemia-reperfusion

Paradis

Charles

Meyer

et al. 2016

American Journal of Physiology-Cell Physiology

103

127

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Peripheral artery disease (PAD) is a common circulatory disorder of the lower limb arteries that reduces functional capacity and quality of life of patients. Despite relatively effective available treatments, PAD is a serious public health issue associated with significant morbidity and mortality. Ischemia-reperfusion (I/R) cycles during PAD are responsible for insufficient oxygen supply, mitochondriopathy, free radical production, and inflammation and lead to events that contribute to myocyte death and remote organ failure. However, the chronology of mitochondrial and cellular events during the ischemic period and at the moment of reperfusion in skeletal muscle fibers has been poorly reviewed. Thus, after a review of the basal myocyte state and normal mitochondrial biology, we discuss the physiopathology of ischemia and reperfusion at the mitochondrial and cellular levels. First we describe the chronology of the deleterious biochemical and mitochondrial mechanisms activated by I/R. Then we discuss skeletal muscle I/R injury in the muscle environment, mitochondrial dynamics, and inflammation. A better understanding of the chronology of the events underlying I/R will allow us to identify key factors in the development of this pathology and point to suitable new therapies. Emerging data on mitochondrial dynamics should help identify new molecular and therapeutic targets and develop protective strategies against PAD.peripheral artery disease; ischemia-reperfusion; skeletal muscle; mitochondria; oxidative stress PERIPHERAL ARTERY DISEASE (PAD) refers to a common circulatory disorder of the lower limb caused by chronic narrowing of the arteries (e.g., stenosis and occlusion) or atherosclerosis. PAD represents a broad spectrum of disease severity, ranging from asymptomatic disease to frequent pain when walking (i.e., intermittent claudication or limping) or critical limb ischemia associated with decubitus pain and/or ulcers (114,126).PAD is known to be associated with reduced functional capacity and quality of life. It is a major cause of limb amputation, as well as an increased risk factor for myocardial infarction, stroke, and death. The incidence of PAD varies with age, from 3-10% in young people to 15-20% in people Ͼ70 yr of age, and is asymptomatic in 40% of the cases (1), with greater prevalence among men. The major PAD risk factors, including smoking, diabetes mellitus, dyslipidemia, hypertension, and obesity, are the same as those for cardiovascular and cerebrovascular diseases (35).Three main complementary treatment options improve the functional status and other clinical outcomes in PAD patients (54). 1) Optimization of medical therapy (i.e., pharmacotherapy) reduces the risk of cardiac ischemia, increases the distance a patient can walk, and improves the functional capacity of patients. 2) When possible, exercise training, a noninvasive and nonpharmacological therapy, improves walking ability and has protective effects in patients with PAD characterized by intermittent claudication and infrainguinal lesions...

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