Metabolic Fingerprinting of Dormant and Active Flower Primordia of <i>Ribes nigrum</i> Using High-Resolution Magic Angle Spinning NMR

Pagter, Majken; Yde, Christian Clement; Kjær, Katrine Heinsvig

doi:10.1021/acs.jafc.7b03788

Cited by 6 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Owing to the huge number of metabolites that could be involved in endodormancy release 24,25 , we have performed, for the first time, a nontargeted analysis during endodormancy in four almond cultivars whose flowering times range from extra-early to ultra-late and whose CR ranges from low (21 CP) to high (56 CP) 26 . As a result, we have been able to identify specific metabolite variations in one or more cultivars.…”

Section: Introductionmentioning

confidence: 99%

Ascorbic acid and prunasin, two candidate biomarkers for endodormancy release in almond flower buds identified by a nontargeted metabolomic study

et al. 2020

View full text Add to dashboard Cite

Temperate fruit trees belonging to Prunus species have the ability to suspend (induce dormancy) and resume growth periodically in response to environmental and seasonal conditions. Endodormancy release requires the long-term accumulation of chill. Upon accumulation of cultivar-specific chill requirements, plants enter the state of ecodormancy, which means the ability to grow has been restored, depending on the fulfilment of heat requirements. As many different metabolic pathways are implicated in endodormancy release, we have performed a metabolomic analysis, using the ultra-high-performance liquid chromatography–quadrupole time-of-flying (UPLC–QToF) technique. We assayed flower buds in different stages of endodormancy in four almond cultivars with different flowering times: the extra-early Desmayo Largueta, the late Antoñeta, the extra-late Penta, and the ultra-late Tardona. An orthogonal projection to latent-structure discriminant-analysis model was created to observe differences between endodormant and ecodormant flower buds. The metabolites showing the most significant variation were searched against the Metlin, HMDB, and KEGG libraries, which allowed us to identify 87 metabolites. These metabolites were subsequently assigned to specific pathways, such as abscisic acid biosynthesis, phenylpropanoid biosynthesis, and D-sorbitol metabolism, among others. The two metabolites that exhibited the most significant variations in all the cultivars studied with fold changes of up to 6.49 were ascorbic acid and prunasin. For the first time, these two metabolites have been proposed as potential biomarkers for endodormancy release in almond. Given the high synteny present between the Rosaceae species, these results could be extrapolated to other important crops like peach, plum, cherry, or apricot, among others.

show abstract

Section: Introductionmentioning

confidence: 99%

Ascorbic acid and prunasin, two candidate biomarkers for endodormancy release in almond flower buds identified by a nontargeted metabolomic study

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Metabolic fingerprinting of flower primordia were analyzed using the HR‐MAS technique, which has previously proved valuable in profiling metabolites of flower primordia (Pagter et al ). Just before measuring respiration, two of the uppermost positioned axillary buds were harvested from five plants per treatment, immediately frozen in liquid nitrogen and stored at −80°C.…”

Section: Methodsmentioning

confidence: 99%

Cold deacclimation mechanisms and reacclimation potential in flower buds of blackcurrant (Ribes nigrum)

Kjær

Winde

Petersen

et al. 2018

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

As a consequence of global climate change, cold acclimation and deacclimation cycles are becoming increasingly frequent during winter in temperate regions. However, little is known about plant deacclimation and in particular reacclimation mechanisms, although deacclimation resistance and the ability to reacclimate may have wide-ranging consequences regarding plant productivity in a changing climate. Here, we report time-dependent responses of freezing tolerance, respiration rates, metabolite contents (high-resolution magic angle spinning NMR) and fatty acid levels (gas chromatography) in flower buds of two ecodormant Ribes nigrum cultivars exposed to three different deacclimation temperatures followed by a reacclimation treatment at 4 ∘ C. The data reveal that despite differences in the progression of deacclimation, the capacity of blackcurrant flower buds to reharden in late winter is virtually non-existing, implying that increasingly irregular temperature patterns is critical for blackcurrant fruit yield. The early phase of deacclimation is associated with a transient increase in respiration and decreasing contents of amino acids, tricarboxylic acid (TCA) cycle intermediates and sugars, indicating an increased need for carbon sources and respiratory energy production for the activation of growth. Decreasing sugar levels may additionally cause loss of freezing tolerance. Deacclimation also involves desaturation of membrane lipids, which likely also contributes to decreased freezing tolerance but may also reflect biosynthesis of signaling molecules stimulating growth and floral organ differentiation. These data provide new insights into the under-researched deacclimation mechanisms and the ability of blackcurrant to reacclimate following different advancements of deacclimation and contribute to our understanding of plant responses to increasingly irregular temperature patterns.

show abstract

“…The HR-MAS NMR-based metabolomics studies in plants have been used for a wide range of applications. The influences of biotic and abiotic stress on the metabolic profile in plants has been widely studied by HR-MAS NMR [41][42][43][44][45][46][47][48][49][50][51][52]. Metabolomics can help in understanding developmental processes, like fruit ripening.…”

Section: Applications Of Hr-mas Nmr In Plant Metabolomicsmentioning

confidence: 99%

HR-MAS NMR Applications in Plant Metabolomics

2021

View full text Add to dashboard Cite

Metabolomics is used to reduce the complexity of plants and to understand the underlying pathways of the plant phenotype. The metabolic profile of plants can be obtained by mass spectrometry or liquid-state NMR. The extraction of metabolites from the sample is necessary for both techniques to obtain the metabolic profile. This extraction step can be eliminated by making use of high-resolution magic angle spinning (HR-MAS) NMR. In this review, an HR-MAS NMR-based workflow is described in more detail, including used pulse sequences in metabolomics. The pre-processing steps of one-dimensional HR-MAS NMR spectra are presented, including spectral alignment, baseline correction, bucketing, normalisation and scaling procedures. We also highlight some of the models which can be used to perform multivariate analysis on the HR-MAS NMR spectra. Finally, applications of HR-MAS NMR in plant metabolomics are described and show that HR-MAS NMR is a powerful tool for plant metabolomics studies.

show abstract

Metabolic Fingerprinting of Dormant and Active Flower Primordia of Ribes nigrum Using High-Resolution Magic Angle Spinning NMR

Cited by 6 publications

References 41 publications

Ascorbic acid and prunasin, two candidate biomarkers for endodormancy release in almond flower buds identified by a nontargeted metabolomic study

Ascorbic acid and prunasin, two candidate biomarkers for endodormancy release in almond flower buds identified by a nontargeted metabolomic study

Cold deacclimation mechanisms and reacclimation potential in flower buds of blackcurrant (Ribes nigrum)

HR-MAS NMR Applications in Plant Metabolomics

Contact Info

Product

Resources

About