Metabolic footprints in phosphate-starved plants

RoyChowdhury, Abhishek; Srivastava, Rajat; Akash, Akash; Shukla, Gyanesh; Zehirov, Grigor; Mishev, Kiril; Kumar, Rahul

doi:10.1007/s12298-023-01319-3

Cited by 7 publications

(2 citation statements)

References 120 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quinate, an organic acid, plays diverse roles, including pH regulation, metal chelation, antioxidant defense, and contributing to carbon allocation strategies in response to challenging nutrient conditions (Carrington et al, 2021). Citrate, a tricarboxylic acid, supports phosphorus solubilization, enhances phosphorus acquisition through root exudation, acidifies the rhizosphere, affects nutrient interactions, and participates in oxidative stress defense (Li et al, 2023; Khan et al, 2023; Roychowdhury et al, 2023). Adenosine monophosphate (AMP) serves as an energy storage molecule, a signaling molecule triggering stress responses, and contributes to metabolic adjustments, promoting adaptability to low phosphorus stress (Min et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Tantriani,

Cheng,

Oikawa

et al. 2023

Physiologia Plantarum

View full text Add to dashboard Cite

Phosphorus (P) deficiency alters the root morphological and physiological traits of plants. This study investigates how soybean cultivars with varying low‐P tolerance values respond to different P levels in hydroponic culture by assessing alterations in root length, acid phosphatase activity, organic acid exudation, and metabolites in root exudates. Three low‐P‐tolerant cultivars (‘Maetsue,’ ‘Kurotome,’ and ‘Fukuyutaka’) and three low‐P‐sensitive cultivars (‘Ihhon,’ ‘Chizuka,’ and ‘Komuta’) were grown under 0 (P0) and 258 μM P (P8) for 7 and 14 days after transplantation (DAT). Low‐P‐tolerant cultivars increased root length by 31% and 119%, which was lower than the 62% and 144% increases in sensitive cultivars under P0 compared to P8 at 7 and 14 DAT, respectively. Acid phosphatase activity in low‐P‐tolerant cultivars exceeded that in sensitive cultivars by 5.2‐fold and 2.0‐fold at 7 and 14 DAT. Root exudates from each cultivar revealed 177 metabolites, with higher organic acid exudation in low‐P‐tolerant than sensitive cultivars under P0. Low‐P‐tolerant cultivars increased concentrations of specific metabolites (oxalate, GABA, quinate, citrate, AMP, 4‐pyridoxate, and CMP), distinguishing them from low‐P‐sensitive cultivars under P0. The top five metabolomic pathways (purine metabolism, arginine and proline metabolism, TCA cycle, glyoxylate and dicarboxylate metabolism, alanine, aspartate, and glutamate metabolism) were more pronounced in low‐P‐tolerant cultivars at 14 DAT. These findings indicate that increasing root length was not an adaptation strategy under P deficiency; instead, tolerant cultivars exhibit enhanced root physiological traits, including increased acid phosphatase activity, organic acid exudation, specific metabolite release, and accelerated metabolic pathways under P deficiency.

show abstract

Section: Discussionmentioning

confidence: 99%

Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Tantriani,

Cheng,

Oikawa

et al. 2023

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…Phosphorus (P), an essential macronutrient, is critical for plant growth and development. P is a necessary constituent of essential biomolecules such as nucleic acids, phospholipids, cellular energy currency, and ATP (Roychowdhury et al, 2023). It also participates in protein phosphorylation and cell signaling events.…”

Section: Introductionmentioning

confidence: 99%

Lack of Purple Acid PhosphataseSlPAP26bcompromises the phosphorus starvation response in tomato independent of SlPHR1 and SlPHL1

Akash,

Srivastava,

Roychowdhury

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

The scarcity of soil phosphorus (P), an essential macronutrient, often limits plant growth and development. Enhanced secretion of intracellular and secretory acid phosphatases is essential to maintain cellular inorganic P (Pi) homeostasis in plants. Herein, using transcriptomics and proteomics approach, we observed upregulation of several purple acid phosphatases (PAPs), includingSlPAP1,SlPAP10b,SlPAP12,SlPAP15,SlPAP17b,SlPAP26a, andSlPAP26bin Pi-deficient tomato seedlings. Higher transcript levels ofSlPAP17bandSlPAP26bin the older senescing leaves than the younger leaves indicated active involvement of these PAPs in Pi remobilization. Subsequent detailed characterization ofSlPAP17b,SlPAP26a, andSlPAP26brevealed a prominent role ofSlPAP26bin Pi homeostasis. Silencing ofSlPAP26bled to an exacerbated P starvation response as these plants exhibited smaller shoots, lower soluble Pi, total P levels, and higher sucrose than their EV controls under Pi deprivation.SlPAP26b-silenced plants also showed misregulation of P starvation inducible genes such as phosphate transporters and glycerolipid remodellers, even under Pi-sufficient conditions. WhereasSlPAP26blevels were induced by external sucrose, its expression was found to be independent of the Myb class master regulators of P starvation response, SlPHR1 and SlPHL1. Altogether, this study identifies a prominent role ofSlPAP26bin the Pi compensation network in tomato seedlings.

show abstract

Underwater light source changes microecosystem constructed by Vallisneria spinulosa Yan for water restoration: Efficiency of water purification, characteristics of growth, and rhizosphere

Wang,

Zhang,

Zhao

et al. 2024

Bioresource Technology

View full text Add to dashboard Cite

Metabolic footprints in phosphate-starved plants

Cited by 7 publications

References 120 publications

Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Lack of Purple Acid PhosphataseSlPAP26bcompromises the phosphorus starvation response in tomato independent of SlPHR1 and SlPHL1

Underwater light source changes microecosystem constructed by Vallisneria spinulosa Yan for water restoration: Efficiency of water purification, characteristics of growth, and rhizosphere

Contact Info

Product

Resources

About