Metabolic glycan labeling and chemoselective functionalization of native biomaterials

Ren, Xi; Evangelista-Leite, Daniele; Wu, Tong; Rajab, Taufiek Konrad; Moser, Philipp T.; Kitano, Kentaro; Economopoulos, Konstantinos P.; Gorman, Daniel E.; Bloom, Jordan P.; Tan, Jun; Gilpin, Sarah E.; Zhou, Haiyang; Mathisen, Douglas J.; Ott, Harald C.

doi:10.1016/j.biomaterials.2018.08.012

Cited by 20 publications

(19 citation statements)

References 44 publications

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“…are supported by scholarships from the China Scholarship Council. This protocol is originally adapted from Ren et al (2018). We thank Pitt Biospecimen Core (University of Pittsburgh) for histological processing.…”

Section: Acknowledgmentsmentioning

confidence: 99%

Bioorthogonal Labeling and Chemoselective Functionalization of Lung Extracellular Matrix

et al. 2021

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Decellularized extracellular matrix (ECM) biomaterials derived from native tissues and organs are widely used for tissue engineering and wound repair. To boost their regenerative potential, ECM biomaterials can be functionalized via the immobilization of bioactive molecules. To enable ECM functionalization in a chemoselective manner, we have recently reported an effective approach for labeling native organ ECM with the click chemistry-reactive azide ligand via physiologic posttranslational glycosylation. Here, using the rat lung as a model, we provide a detailed protocol for in vivo and ex vivo metabolic azide labeling of the native organ ECM using N-Azidoacetylgalactosaminetetraacylated (Ac4GalNAz), together with procedures for decellularization and labeling characterization.Our approach enables specific and robust ECM labeling within three days in vivo or within one day during ex vivo organ culture. The resulting ECM labeling remains stable following decellularization. With our approach, ECM biomaterials can be functionalized with desired alkyne-modified biomolecules, such as growth factors and glycosaminoglycans, for tissue engineering and regenerative applications.

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Section: Acknowledgmentsmentioning

confidence: 99%

Bioorthogonal Labeling and Chemoselective Functionalization of Lung Extracellular Matrix

et al. 2021

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“…In contrast, modifications of the scaffold ex vivo may include molecular refinement of the scaffold using covalent or noncovalent chemistries. These molecular refinements could change the material properties of the scaffold to modify the compliance of bioartificial lungs or confer resistance to bacterial infections, for example 125 …”

Section: Applications For Tissue‐engineered Scaffolding From Organ Anmentioning

confidence: 99%

“…These molecular refinements could change the material properties of the scaffold to modify the compliance of bioartificial lungs or confer resistance to bacterial infections, for example. 125…”

Section: Tissue-engineered Scaffolding From Organ and Tissue Decellulmentioning

confidence: 99%

Decellularized scaffolds for tissue engineering: Current status and future perspective

Rajab¹,

O’Malley

Tchantchaleishvili

2020

Artificial Organs

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Based on Organ Procurement and Transplantation Network data as of December 2019, more than 113 000 patients require an organ transplantation, yet, over the course of this past year, only 36 000 patients received a transplant. This discrepancy between those that need a donor organ and those that receive one remains one of medicine’s biggest challenges. Multiple solutions, both biologic and artificial, have been proposed to mitigate this difference. One of the most promising approaches to generate bioartificial organs for transplantation involves re‐seeding decellularized scaffolds with appropriate cells. Decellularization involves physical, chemical, or biological methods that typically require intimate contact of various decellularization solutions with each cell. Consequently, conventional submersion decellularization has been limited to simple tissues such as heart valves. The invention of perfusion decellularization was a breakthrough that allowed the generation of tissue‐engineered scaffolds from tissues with higher structural organization and entire organs. Such scaffolds are composed of a myriad of extracellular matrix (ECM) components that include collagen, elastin, proteoglycans, and glycoproteins. Together, these components allow the scaffolds to fulfill specialized functions, such as structural functions as well as biological functions including the regulation of cellular processes and extracellular molecules. These specialized functions of decellularized scaffolds can increasingly be harnessed for applications in tissue engineering.

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“…[ 37,62,63 ] On the other end, introduction of bio‐orthogonal functionalities at the cell membrane offers clear advantages over nonspecific polymer conjugation, including better cytocompatibility and stability of conjugation. [ 64–66 ] The cell surface repertoire can be expanded to include abiotic functionality through the biosynthetic introduction of unnatural sugars into cellular glycans, a process termed metabolic oligosaccharide engineering (MOE). [ 13,67,68 ] This method was pioneered by Bertozzi and co‐workers and allows easy incorporation of bio‐orthogonal handles in vitro and in vivo.…”

Section: Covalent Conjugation Using Bio‐orthogonal Chemistrymentioning

confidence: 99%

Engineering the Mammalian Cell Surface with Synthetic Polymers: Strategies and Applications

Arno

2020

Macromol. Rapid Commun.

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Manipulating the surface of living cells represents a powerful tool by which to control cell behavior and provides a unique strategy to modulate cellular function and cell–cell interactions. Recent progress in this area has seen the development of robust and elegant approaches to selectively decorate the cell surface, leading to unprecedented advances in cellular manipulation and cell‐based therapies. Despite some impressive in vitro results, several obstacles remain to the broader application of some of these strategies, including their limited translation in vivo. In this review, the leading techniques used to introduce polymers at the plasma membrane of mammalian cells are discussed, focusing on strategies that generate a stable and homogeneous distribution of polymeric chains at the cell surface. Application of these strategies to control cell behavior and deliver cell‐based therapies to targeted tissues are highlighted.

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Metabolic glycan labeling and chemoselective functionalization of native biomaterials

Cited by 20 publications

References 44 publications

Bioorthogonal Labeling and Chemoselective Functionalization of Lung Extracellular Matrix

Bioorthogonal Labeling and Chemoselective Functionalization of Lung Extracellular Matrix

Decellularized scaffolds for tissue engineering: Current status and future perspective

Engineering the Mammalian Cell Surface with Synthetic Polymers: Strategies and Applications

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