Metabolic improvements and use of inexpensive carbon sources in microbial production of polyhydroxyalkanoates

Tsuge, Takeharu

doi:10.1016/s1389-1723(02)80198-0

Cited by 209 publications

(103 citation statements)

References 29 publications

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“…Within the bacteria, the polymers are present in the form of spherical granules, generally 50-500 nm in diameter. Extracellular PHA is often crystalline having about 50-80 % of crystallinity [56,84]. The crystallinity of PHA, which depends on M w and monomer composition, in turn, affects its thermal, mechanical properties and biodegradability [85].…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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Polyhydroxyalkanoates (PHAs) as an alternative to synthetic plastics have been gaining increasing attention. Being natural in their origin, PHAs are completely biodegradable and eco-friendly. However, consistent efforts to exploit this biopolymer over the last few decades have not been able to pull PHAs out of their nascent stage, inspite of being the favorite of the commercial world. The major limitations are: (1) the high production cost, which is due to the high cost of the feed and (2) poor thermal and mechanical properties of polyhydroxybutyrate (PHB), the most commonly produced PHAs. PHAs have the physicochemical properties which are quite comparable to petroleum based plastics, but PHB being homopolymers are quite brittle, less elastic and have thermal properties which are not suitable for processing them into sturdy products. These properties, including melting point (T m ), glass transition temperature (T g ), elastic modulus, tensile strength, elongation etc. can be improved by varying the monomeric composition and molecular weight. These enhanced characteristics can be achieved by modifications in the types of substrates, feeding strategies, culture conditions and/or genetic manipulations.

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Section: Mechanical Propertiesmentioning

confidence: 99%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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“…Polyhydroxyalkanoates (PHAs) are a class of storage polymers synthesized by various microorganisms (1). These polymers have been considered as alternatives to petroleum-derived materials.…”

Section: Introductionmentioning

confidence: 99%

“…These polymers have been considered as alternatives to petroleum-derived materials. However, commercialization efforts of these polymers have been hampered by high production cost, significantly contributed by the carbon sources used for their production (1,2). Therefore, it is essential to use inexpensive carbon substrates for the production of PHAs so as to render them cost competitive.…”

Section: Introductionmentioning

confidence: 99%

“…Here, we attempted to produce P(3HB-co-3HV) from cellulose hydrolysate as a typical example to demonstrate the potential application of E. coli LS5218. P(3HB-co-3HV) is an attractive polymer as incorporation of the 3HV fraction to approximately 20% improves the strength and flexibility of the polymer compared to P(3HB) homopolymer which is highly crystalline and brittle (1,8).…”

Section: Introductionmentioning

confidence: 99%

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Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance to 5-hydroxymethylfurfural

Nduko

Suzuki

Matsumoto

et al. 2012

Journal of Bioscience and Bioengineering

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Poly[3-hydroxybutyrate-co-3-hydroxyvalerate (3HV)] was produced in recombinant Escherichia coli LS5218 from the ruthenium-catalyzed cellulose hydrolysate and propionate. The strain was found to be resistant against 5-hydroxymethylfurfural (5-HMF), which is a major inhibitory byproduct generated in the cellulose hydrolysis reaction. The 3HV fraction was successfully regulated in the range of 5.6 -40 mol%.

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“…4). Although the composition of the PHA granule has been reported to be altered depending on the carbon source (Tsuge, 2002), in this study we assumed that the observed granules in the NT80 cells were made of PHAs, and therefore we used PHB to establish the calibration curve. It has been reported that PHA influences biofilm formation (Tribelli & Ló pez, 2011).…”

Section: Stearyl Alcohol Induces Pha Productionmentioning

confidence: 99%

EliA is required for inducing the stearyl alcohol-mediated expression of secretory proteins and production of polyester in Ralstonia sp. NT80

et al. 2016

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Addition of stearyl alcohol to the culture medium of Ralstonia sp. NT80 induced expression of a significant amount of secretory lipase. Comparative proteomic analysis of extracellular proteins from NT80 cells grown in the presence or absence of stearyl alcohol revealed that stearyl alcohol induced expression of several secretory proteins including lipase, haemolysin-coregulated protein and nucleoside diphosphate kinase. Expression of these secreted proteins was upregulated at the transcriptional level. Stearyl alcohol also induced the synthesis of polyhydroxyalkanoate. Secretory protein EliA was required for all these responses of NT80 cells to stearyl alcohol. Accordingly, the effects of stearyl alcohol were significantly reduced in the eliA deletion mutant cells of NT80 (DeliA). The remaining concentration of stearyl alcohol in the culture supernatant of the wild-type cells, but not that in the culture supernatant of the DeliA cells, clearly decreased during the course of growth. These observed phenotypes of the DeliA mutant were rescued by gene complementation. The results suggested that EliA is essential for these cells to respond to stearyl alcohol, and that it plays an important role in the recognition and assimilation of stearyl alcohol by NT80 cells.

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Metabolic improvements and use of inexpensive carbon sources in microbial production of polyhydroxyalkanoates

Cited by 209 publications

References 29 publications

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance to 5-hydroxymethylfurfural

EliA is required for inducing the stearyl alcohol-mediated expression of secretory proteins and production of polyester in Ralstonia sp. NT80

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