Metabolic incorporation of unsaturated fatty acids into boar spermatozoa lipids and de novo formation of diacylglycerols

Svetlichnyy, Valentin; Müller, Peter; Pomorski, Thomas; Schulze, Martin; Schiller, Jürgen; Müller, Karin

doi:10.1016/j.chemphyslip.2013.11.001

Cited by 12 publications

(3 citation statements)

References 36 publications

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“…For instance, addition of fish-oil to the diet can increase the n-3/n-6 ratio of PUFAs in boar sperm [ 34 ], but it is also described that a dietary increase in docosahexaenoic acid in sperm phospholipids is counterbalanced by a decrease in docosapentaenoic acid [ 35 ]. Moreover, only unsaturated FA that are endogenously present may be metabolically incorporated into boar sperm lipids in vitro [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Seminal lipid profiling and antioxidant capacity: A species comparison

Jakop

Müller

et al. 2022

PLoS ONE

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On their way to the oocyte, sperm cells are subjected to oxidative stress, which may trigger the oxidation of phospholipids (PL). Applying MALDI-TOF MS, HPTLC and ESI-IT MS, we comparatively analyzed the PL compositions of semen and blood of species differing in their reproductive systems and types of nutrition (bull, boar, stallion, lion and man) with regard to the sensitivity to oxidation as well as the accumulation of harmful lyso-PL (LPL), transient products of lipid oxidation. In addition, the protective capacity of seminal fluid (SF) was also examined. The PL composition of erythrocytes and blood plasma is similar across the species, while pronounced differences exist for sperm and SF. Since the blood function is largely conserved across mammalian species, but the reproductive systems may vary in many aspects, the obtained results suggest that the PL composition is not determined by the type of nutrition, but by the relatedness of species and by functional requirements of cell membranes such as fluidity. Sperm motion and fertilization of oocytes require a rather flexible membrane, which is accomplished by significant moieties of unsaturated fatty acyl residues in sperm lipids of most species, but implies a higher risk of oxidation. Due to a high content of plasmalogens (alkenyl ether lipids), bull sperm are most susceptible to oxidation. Our data indicate that bull sperm possess the most effective protective power in SF. Obviously, a co-evolution of PL composition and protective mechanisms has occurred in semen and is related to the reproductive characteristics. Although the protective capacity in human SF seems well developed, we recorded the most pronounced individual contaminations with LPL in human semen. Probably, massive oxidative challenges related to lifestyle factors interfere with natural conditions.

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Section: Discussionmentioning

confidence: 99%

Seminal lipid profiling and antioxidant capacity: A species comparison

Jakop

Müller

et al. 2022

PLoS ONE

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“…The plasma membrane of spermatozoa commonly comprises 70% phospholipids, 25% neutral lipids and 5% glycolipids (Mann & Lutwak‐Mann, 2012). In addition, it was found that spermatozoa can synthesize lipids, including phosphatidylcholine de novo (Svetlichnyy et al., 2014). The plasma membrane of sperm is a crucial component that enables the sperm to respond to physiological stimuli and protects against extracellular damage.…”

Section: Discussionmentioning

confidence: 99%

Low concentrations of soybean lecithin nanoparticles had a positive impact on Holstein bulls' cryopreserved semen

Abdel‐Ghani,

El‐Sharawy,

Zaid

et al. 2024

Reprod Domestic Animals

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Spermatozoa can experience negative changes when subjected to freezing and thawing, including lowered motility, viability and acrosome response. Herein, the effects of different concentrations of soybean lecithin nanoparticles on cryopreserved Holstein bull semen were examined. Semen was collected, cryopreserved and utilized for sperm kinetic parameter analysis following dilution, equilibration and thawing with 0.5% soybean lecithin (E1), the control extender, and 0.75% (E2), 0.5% (E3), 0.25% (E4) and 0.125% (E5) of lecithin nanoparticles. Results revealed that following dilution, the progressive motility (PM) at E3, E4 and E5 of lecithin nanoparticles was higher (p < .05) than it was for E2. After equilibration, compared to the E1, E2, and E3 values, the PM, vitality, normal morphology, membrane integrity and intact acrosome values at the E5 were consistently greater (p < .05). Comparing the percentages of intact acrosome and membrane integrity at E2 and E3 to E4 and E5, a substantial decrease (p < .05) was seen. Following thawing, the percentage of PM improved at E2 and E5, even though their mean PM values were similar (p > .05) compared to E1, E3 and E4. Vigour and progression parameters of sperm (DAP, DCL, DSL, VAP, VCL, VSL and STR) at E5 were higher (p < .05) than those at E1, E2, E3 and E4. In conclusion, the cryopreserved sperm from Holstein bulls revealed outstanding properties both after equilibration and after thawing with 0.125% lecithin nanoparticles, and they were sensitive to high dosages.

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“…Spermatozoa possess glycolytic and respiratory capacities, which are predominantly related to the need to maintain cell motility [2]. Additionally, as research progressed, spermatozoa were found to feature lipid synthesis, such as de novo synthesis of phosphatidylcholine [2,3]. The organization of eukaryotic cells depends to a large extent on the structure and function of membranes, based upon the intrinsic properties of membrane lipid components [4].…”

Section: Introductionmentioning

confidence: 99%

Sperm Lipid Markers of Male Fertility in Mammals

Shan

Hirschfeld

et al. 2021

IJMS

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Sperm plasma membrane lipids are essential for the function and integrity of mammalian spermatozoa. Various lipid types are involved in each key step within the fertilization process in their own yet coordinated way. The balance between lipid metabolism is tightly regulated to ensure physiological cellular processes, especially referring to crucial steps such as sperm motility, capacitation, acrosome reaction or fusion. At the same time, it has been shown that male reproductive function depends on the homeostasis of sperm lipids. Here, we review the effects of phospholipid, neutral lipid and glycolipid homeostasis on sperm fertilization function and male fertility in mammals.

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Metabolic incorporation of unsaturated fatty acids into boar spermatozoa lipids and de novo formation of diacylglycerols

Cited by 12 publications

References 36 publications

Seminal lipid profiling and antioxidant capacity: A species comparison

Seminal lipid profiling and antioxidant capacity: A species comparison

Low concentrations of soybean lecithin nanoparticles had a positive impact on Holstein bulls' cryopreserved semen

Sperm Lipid Markers of Male Fertility in Mammals

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