Metabolic Insights Into Infochemicals Induced Colony Formation and Flocculation in Scenedesmus subspicatus Unraveled by Quantitative Proteomics

Roccuzzo, Sebastiana; Couto, Narciso; Karunakaran, Esther; Kapoore, Rahul Vijay; Butler, Thomas O.; Mukherjee, Joy; Hansson, Erika M; Beckerman, Andrew P.; Pandhal, Jagroop

doi:10.3389/fmicb.2020.00792

Cited by 22 publications

(20 citation statements)

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“…The molecular processes behind the response of D. subspicatus to produce colonies and form aggregates as an adaptive response to Daphnia cues have been unraveled recently (Roccuzzo et al, 2020). These authors identified a role of the mitogen-activated protein kinase (MAPK) phosphatases signaling pathway in the response of D. subspicatus to Daphnia infochemicals.…”

Section: Costs and Benefits Of Phytoplankton Defensesmentioning

confidence: 99%

Grazing resistance in phytoplankton

Lürling

2020

Hydrobiologia

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Phytoplankton is confronted with a variable assemblage of zooplankton grazers that create a strong selection pressure for traits that reduce mortality. Phytoplankton is, however, also challenged to remain suspended and to acquire sufficient resources for growth. Consequently, phytoplanktic organisms have evolved a variety of strategies to survive in a variable environment. An overview is presented of the various phytoplankton defense strategies, and costs and benefits of phytoplankton defenses with a zooming in on grazer-induced colony formation. The tradeoff between phytoplankton competitive abilities and defenses against grazing favor adaptive trait changes-rapid evolution and phenotypic plasticity-that have the potential to influence population and community dynamics, as exemplified by controlled chemostat experiments. An interspecific defense-growth trade-off could explain seasonal shifts in the species composition of an in situ phytoplankton community yielding defense and growth rate as key traits of the phytoplankton. The importance of grazing and protection against grazing in shaping the phytoplankton community structure should not be underestimated. The trade-offs between nutrient acquisition, remaining suspended, and grazing resistance generate the dynamic phytoplankton community composition.

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Section: Costs and Benefits Of Phytoplankton Defensesmentioning

confidence: 99%

Grazing resistance in phytoplankton

Lürling

2020

Hydrobiologia

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“…The microalgal cells are round and form aggregates through cell-cell contacts (Fig. 1), which is different from other reported Scenedesmus strains that are in spindle shape [12]. Fig.…”

Section: Morphological Features and Genome Assemblymentioning

confidence: 59%

“…On the other hand, some microalgal strains occulate or aggregate spontaneously [9][10][11]. The self-occulation of microalgal cells enables their harvest without the addition of exogenous occulants, and biomass harvesting based on the self-occulation of microalgal cells is more environmentally friendly compared to the occulation of microalgal cells through physical and chemical methods, or induced by infochemicals from predators [8,12].…”

Section: Introductionmentioning

confidence: 99%

Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

Chen

Mhuantong²,

et al. 2020

Preprint

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Background Scenedesmus obliquus belongs to green microalgae and is widely used in aquaculture as feed, which is also explored for lipid production and bioremediation. However, genomic studies of this microalga have been very limited. Cell self-flocculation of microalgal cells can be used as a simple and economic method for harvesting biomass, and it is of great importance to perform genome-scale studies for the self-flocculating S. obliquus strains to promote their biotechnological applications. Results We sequenced the genome of the self-flocculating microalga S. obliquus AS-6-11 employing the Pacific Biosciences sequencing platform, and used the MECAT software for de novo genome assembly. The estimated genome size of S. obliquus AS-6-11 is 172.3 Mbp with an N50 of 94,410 bp, and 31,964 protein-coding genes were identified. Gene Ontology (GO) and KEGG pathway analysis revealed 65 GO terms and 428 biosynthetic pathways. Comparing to the well-studied green microalgae Chlamydomonas reinhardtii, Chlorella variabilis, Volvox carteri and Micractinium conductrix, the genome of S. obliquus AS-6-11 encodes more unique proteins, and some of them might be responsible for the self-flocculating phenotype. These genes encode D-mannose binding lectin, glycosylphosphatidylinositol (GPI)-anchored cell wall proteins, and proteins with fasciclin domains that are commonly found in cell surface proteins, respectively. Four genes encoding both GPI-anchored cell wall proteins and fasciclin domain proteins are the most interesting targets for further studies. Conclusions To our best knowledge, this is the first report on the in-depth annotation of the S. obliquus genome, and the results will facilitate functional genomic studies of this important microalga. The comparative genomic analysis here also provides new insights into the evolution of green microalgae. Furthermore, identification of the potential genes encoding proteins for the self-flocculation of microalgal cells will benefit studies on the molecular mechanism underlying this phenotype for its better control and biotechnological applications as well.

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“…The self-occulation of microalgal cells enables their harvest without the addition of exogenous occulants, and biomass harvesting based on the self-occulation of microalgal cells is more environmentally friendly compared to the occulation of microalgal cells through physical and chemical methods, or induced by infochemicals from predators [8,12].…”

Section: Introductionmentioning

confidence: 99%

Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

Chen

Mhuantong²,

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Scenedesmus obliquus belongs to green microalgae and is widely used in aquaculture as feed, which is also explored for lipid production and bioremediation. However, genomic studies of this microalga have been very limited. Cell self-flocculation of microalgal cells can be used as a simple and economic method for harvesting biomass, and it is of great importance to perform genome-scale studies for the self-flocculating S. obliquus strains to promote their biotechnological applications.Results: We employed the Pacific Biosciences sequencing platform for sequencing the genome of the self-flocculating microalga S. obliquus AS-6-11, and used the MECAT software for de novo genome assembly. The estimated genome size of S. obliquus AS-6-11 is 172.3 Mbp with an N50 of 94,410 bp, and 31,964 protein-coding genes were identified. Gene Ontology (GO) and KEGG pathway analyses revealed 65 GO terms and 428 biosynthetic pathways. Comparing to the genome sequences of the well-studied green microalgae Chlamydomonas reinhardtii, Chlorella variabilis, Volvox carteri and Micractinium conductrix, the genome of S. obliquus AS-6-11 encodes more unique proteins, including one gene that encodes D-mannose binding lectin. Genes encoding the glycosylphosphatidylinositol (GPI)-anchored cell wall proteins, and proteins with fasciclin domains that are commonly found in cell wall proteins might be responsible for the self-flocculating phenotype, and were analyzed in detail. Four genes encoding both GPI-anchored cell wall proteins and fasciclin domain proteins are the most interesting targets for further studies.Conclusions: To our best knowledge, this is the first report on the in-depth annotation of the S. obliquus genome, and the results will facilitate functional genomic studies and metabolic engineering of this important microalga. The comparative genomic analysis here also provides new insights into the evolution of green microalgae. Furthermore, identification of the potential genes encoding self-flocculating proteins will benefit studies on the molecular mechanism underlying this phenotype for its better control and biotechnological applications as well.

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Metabolic Insights Into Infochemicals Induced Colony Formation and Flocculation in Scenedesmus subspicatus Unraveled by Quantitative Proteomics

Cited by 22 publications

References 84 publications

Grazing resistance in phytoplankton

Grazing resistance in phytoplankton

Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

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