Metabolic Markers of Embryo Viability

Donnay, Isabelle

doi:10.1007/978-94-010-0343-8_3

Cited by 9 publications

(5 citation statements)

References 134 publications

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“…The use of a determined set of genes covering a broad range of physiological systems in the embryos to assess the quality of the in vitro production system will be instrumental in improving the quality of embryos produced (Wrenzycki et al. 1998; Donnay 2002). As far as we know, this is the first study to compare expression levels of retinoid X receptors between different quality cattle embryos.…”

Section: Discussionmentioning

confidence: 99%

Expression of Retinoid X Receptor Transcripts and their Significance for Developmental Competence in In Vitro‐produced Pre‐implantation‐stage Bovine Embryos

Mamo

Ponsuksili

Wimmers

et al. 2005

Reprod Domestic Animals

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Retinoid X receptors are frequently implicated in their role in embryonic development. However, there is no report regarding their expression in embryos with different qualities. In this study, expression pattern and levels of retinoid X receptor transcripts in different qualities and stages of in vitro-produced pre-implantation bovine embryos were examined using real-time polymerase chain reaction (PCR) techniques. Four independent cDNA pools per sample were prepared from mRNAs isolated from different developmental-stage embryos, after classifying them to be of good and bad quality. Primers were also designed to amplify products with PCR. Based on analysis, except retinoid X receptor gamma (RXRG) transcripts, the remaining two types (alpha and beta forms) were expressed in all samples with significantly higher (p < or = 0.05) levels until the eight-cell stage compared with the stages thereafter. However, the transcript levels for bad-quality embryos were consistently lower in all cases in comparison with good-quality embryos. RXRG was identified in 39% of the samples, but all in the pre-embryonic genome activation development stage. Therefore, strong expression patterns of these transcripts in earlier stages indicates their possible role during the maternal phase of embryo development, and the variations of copy number in embryos with different qualities substantiates their possible candidacy as potential quality markers. Moreover, identifying the transcript variations in different qualities and expression of RXRG at these stages is a novel input to the pre-implantation-stage knowledge.

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Section: Discussionmentioning

confidence: 99%

Expression of Retinoid X Receptor Transcripts and their Significance for Developmental Competence in In Vitro‐produced Pre‐implantation‐stage Bovine Embryos

Mamo

Ponsuksili

Wimmers

et al. 2005

Reprod Domestic Animals

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“…The metabolic profile for a specific metabolite can be measured as the quantity (i) consumed/taken up or (ii) released by the embryo. The evaluation of the embryo microenvironment, i.e., spent culture media, can give a reasonably accurate picture of both of the above measurements for individual metabolites or the entire metabolome [ 10 , 23 , 211 ].…”

Section: Embryo Evaluation/analysis—tools Of the Tradementioning

confidence: 99%

“…For both IVP and IVD embryos, it is well established that the transfer of higher-quality blastocysts results in higher PRs compared to the transfer of lower-quality blastocysts [ 7 , 8 , 9 ]. Therefore, if embryos can be accurately evaluated and the high-quality ones could be selected for ET, the commercial value of selected embryos would increase, with a subsequent decrease in the number of recipients and an overall improvement in the efficiency of ET [ 10 ]. Therefore, one of the most important factors associated with the successful application of ARTs is embryo evaluation prior to freezing or transfer to recipients [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Pre-Implantation Bovine Embryo Evaluation—From Optics to Omics and Beyond

Rabel

Marchioretto

Bangert

et al. 2023

Animals

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Approximately 80% of the ~1.5 million bovine embryos transferred in 2021 were in vitro produced. However, only ~27% of the transferred IVP embryos will result in live births. The ~73% pregnancy failures are partly due to transferring poor-quality embryos, a result of erroneous stereomicroscopy-based morphological evaluation, the current method of choice for pre-transfer embryo evaluation. Numerous microscopic (e.g., differential interference contrast, electron, fluorescent, time-lapse, and artificial-intelligence-based microscopy) and non-microscopic (e.g., genomics, transcriptomics, epigenomics, proteomics, metabolomics, and nuclear magnetic resonance) methodologies have been tested to find an embryo evaluation technique that is superior to morphologic evaluation. Many of these research tools can accurately determine embryo quality/viability; however, most are invasive, expensive, laborious, technically sophisticated, and/or time-consuming, making them futile in the context of in-field embryo evaluation. However accurate they may be, using complex methods, such as RNA sequencing, SNP chips, mass spectrometry, and multiphoton microscopy, at thousands of embryo production/collection facilities is impractical. Therefore, future research is warranted to innovate field-friendly, simple benchtop tests using findings already available, particularly from omics-based research methodologies. Time-lapse monitoring and artificial-intelligence-based automated image analysis also have the potential for accurate embryo evaluation; however, further research is warranted to innovate economically feasible options for in-field applications.

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“…In this case, very low oxygen consumptive rate would correlate with adverse alterations in oxidative phosphorylation and in other oxygen-related processes (Ziebe et al, 1997), metabolic stress and consequently with reduced embryo viability and increased mortality. Furthermore, it could be expected that the culture conditions and media components such as glucose, amino acids, pyruvate and serum, which are known to affect embryo metabolism (Eckert et al, 1998;Lane and Gardner, 2000;Donnay, 2002;Abe et al, 2005), would have had quantifiable…”

Section: The Expression Of Apoptosis-related Genesmentioning

confidence: 99%

Influence of Oxygen Consumption on Pregnancy Rates of Hanwoo Calves following Embryo Transfer

Kim

Bok

Kim

et al. 2014

J Anim Reprod Biotechnol

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Recently, several approaches have been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygen consumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, we investigated the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo using a SECM. In addition to, the expression of apoptosis-related genes was determined using real-time PCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygen consumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos, indicating that oxygen consumption reflects the embryo quality (Grade I). The oxygen consumption or GI blastocysts were significantly higher than those of GⅡ blastocysts (10.2×10 14 /mol s -1 versus 6.4×10 14 /mol s -1 , p< 0.05). Pregnant rate in recipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0∼12.0 and over 12.0 × 10 14 /mol s -1 , respectively. Apoptosis regulatory genes, Hsp-70.1 were significantly increased in over-10.0 group than below 10.0 group but in Caspase-3, Bax and P53 gene, there was no significant difference. In conclusion, These results suggest that measurement of oxygen consumption maybe help increase the pregnant rate of Hanwoo embryos.

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Metabolic Markers of Embryo Viability

Cited by 9 publications

References 134 publications

Expression of Retinoid X Receptor Transcripts and their Significance for Developmental Competence in In Vitro‐produced Pre‐implantation‐stage Bovine Embryos

Expression of Retinoid X Receptor Transcripts and their Significance for Developmental Competence in In Vitro‐produced Pre‐implantation‐stage Bovine Embryos

Pre-Implantation Bovine Embryo Evaluation—From Optics to Omics and Beyond

Influence of Oxygen Consumption on Pregnancy Rates of Hanwoo Calves following Embryo Transfer

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