Metabolic mechanisms of Coilia nasus in the natural food intake state during migration

Yin, Denghua; Lin, Danqing; Ying, Congping; Ma, Fengjiao; Yang, Yanping; Wang, Yinping; Tan, Jinhao; Li, Kai

doi:10.1016/j.ygeno.2020.05.027

Cited by 13 publications

(9 citation statements)

References 72 publications

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“…In addition, studies of the metabolism of the anchovy Coilia nasus in their natural feeding state during migration have shown that females make up a large percentage of the feeding group during migration. When migrating closer, females expend more energy relative to males due to ovarian development, and a larger proportion of feeding individuals are females [84]. The results of this study of hard-shelled mussels showed that the expression of HCRTR2 in the gonads of the female was higher than that of males, which was consistent with the results of the C. nasus study above.…”

Section: Key Differentially Expressed Genessupporting

confidence: 89%

Transcriptome Sequencing Analysis of Sex-Related Genes in the Gonads of Mytilus unguiculatus

Ma,

Ye,

Yao

et al. 2023

Fishes

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In the cultivation of Mytilus unguiculatus, the broodstock are shade-dried to stimulate sperm and egg production. To identify the functional genes affecting gonad development in M. unguiculatus, the transcriptome of gonads in mussels stimulated by shade-drying and those not stimulated were compared. Differential gene expression analysis revealed that there were 22 differentially expressed genes (DEGs) in the testis and 70 DEGs in the ovary among the experimental groups. KEGG enrichment analysis identified a total of 11 pathways that might be related to environmental stimuli affecting gonadal development. Nicotinic acetylcholine receptors (AChRs), the cholecystokinin A receptor (CCKAR), hypocretin (orexin) receptor 2 (HCRTR2), and gamma-aminobutyric acid type B receptor (GABBR) were highly expressed in the neuroactive ligand-receptor interaction pathway, indicating that these genes might be involved in the transduction of environmental information that stimulates gonadal development. Meanwhile, nuclear receptor co-repressor 2 (NCoR2) was highly expressed in the notch signaling pathway, indicating that NCoR2 might be involved in the regulation of gonad development. To validate the transcriptome data, we selected five DEGs in the KEGG signaling pathway, including AChRs, CCKAR, HCRTR2, GABAB, and NCoR2, for real-time quantitative PCR (RT-qPCR), which produced results consistent with the RNA-Seq data. The transcriptome analysis and gene pathway identification in this study have enhanced our comprehension of the reproductive mechanisms in M. unguiculatus.

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Section: Key Differentially Expressed Genessupporting

confidence: 89%

Transcriptome Sequencing Analysis of Sex-Related Genes in the Gonads of Mytilus unguiculatus

Ma,

Ye,

Yao

et al. 2023

Fishes

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“…Colonic samples were stored overnight at −20°C for metabolite detection according to the manufacture’s standard protocol ( Yin et al, 2020 ). Briefly, samples were acquired by using the LC-MS system and were performed by a Thermo Scientific UltiMate 3000 HPLC followed by machine orders.…”

Section: Methodsmentioning

confidence: 99%

Changes in Serum Fatty Acid Composition and Metabolome-Microbiome Responses of Heigai Pigs Induced by Dietary N-6/n-3 Polyunsaturated Fatty Acid Ratio

Wang

Nong²,

Zhou³

et al. 2022

Front. Microbiol.

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Changing fatty acid composition is a potential nutritional strategy to shape microbial communities in pigs. However, the effect of different n-6/n-3 polyunsaturated fatty acid (PUFA) ratios on serum fatty acid composition, microbiota, and their metabolites in the intestine of pigs remains unclear. Our study investigated the changes in serum fatty acid composition and metabolome–microbiome responses induced by dietary n-6/n-3 PUFA ratio based on a Heigai-pig model. A total of 54 Heigai finishing pigs (body weight: 71.59 ± 2.16 kg) fed with 3 types of diets (n-6/n-3 PUFA ratios are 8:1, 5:1, and 3:1) were randomly divided into 3 treatments with 6 replications (3 pigs per replication) for 75 days. Results showed that dietary n-6/n-3 PUFA ratio significantly affected biochemical immune indexes including glucose (Glu), triglycerides (TG), total cholesterol (TChol), non-esterified fatty acid (NEFA), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and total thyroxine (TT4), and medium- and long-chain fatty acid composition, especially n-3 PUFA and n-6/n-3 PUFA ratio in the serum. However, no significant effects were found in the SCFAs composition and overall composition of the gut microbiota community. In the low dietary n-6/n-3 PUFA ratio group, the relative abundance of Cellulosilyticum, Bacteroides, and Alloprevotella decreased, Slackia and Sporobacter increased. Based on the metabolomic analysis, dietary n-6/n-3 PUFA ratio altered the metabolome profiles in the colon. Moreover, Pearson’s correlation analysis indicated that differential microbial genera and metabolites induced by different n-6/n-3 PUFA ratio had tight correlations and were correlated with the n-6 PUFA and n-3 PUFA content in longissimus dorsi muscle (LDM) and subcutaneous adipose tissue (SAT). Taken together, these results showed that lower dietary n-6/n-3 PUFA ratio improved serum fatty acid composition and metabolome–microbiome responses of Heigai pigs and may provide a new insight into regulating the metabolism of pigs and further better understanding the crosstalk with host and microbes in pigs.

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“…For the anadromous C. nasus , the ovarian transcriptome profiles revealed a large proportion of genes involved in energy production, amino acid transport and metabolism during spawning migration ( Duan et al, 2015 ) via food intake for energy consumption and energy storage ( Yin et al, 2020 ). Brain transcriptome analyses of C. nasus from the Yangtze River showed that the brain plays a more regulatory role during migration, and the signal transduction pathways and genes relevant to neurotransmitter receptors, hormones and KISS1R were upregulated, providing key regulatory genes for gonadal development of anadromous C. nasus ( Wang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

A delayed and unsynchronized ovary development as revealed by transcriptome of brain and pituitary of Coilia nasus

Yu,

Gao,

Zeng

et al. 2024

Front. Mol. Biosci.

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Coilia nasus is an anadromous fish that has been successfully domesticated in the last decade due to its high economic value. The fish exhibits a delayed ovary development during the reproductive season, despite breeding and selection for five to six offspring. The molecular mechanism of the delayed ovary development is still unknown, so the obstacles have not been removed in the large-scale breeding program. This study aims to investigate the key genes regulating ovarian development by comparing the transcriptomes of ovarian-stage IV and stage II brain/pituitary of Coilia nasus. Ovarian stages were validated by histological sections. A total of 75,097,641 and 66,735,592 high-quality reads were obtained from brain and pituitary transcriptomes, respectively, and alternatively spliced transcripts associated with gonadal development were detected. Compared to ovarian Ⅱ- brain, 515 differentially expressed genes (DEGs) were upregulated and 535 DEGs were downregulated in ovarian Ⅳ- brain, whereas 470 DEGs were upregulated and 483 DEGs were downregulated in ovarian Ⅳ- pituitary compared to ovarian Ⅱ- pituitary. DEGs involved in hormone synthesis and secretion and in the GnRH signaling pathway were screened. Weighted gene co-expression network analysis identified gene co-expression modules that were positively correlated with ovarian phenotypic traits. The hub genes Smad4 and TRPC4 in the modules were co-expressed with DEGs including Kiss1 receptor and JUNB, suggesting that ovarian development is controlled by a hypothalamic-pituitary-gonadal axis. Our results have provided new insights that advance our understanding of the molecular mechanism of C. nasus reproductive functions and will be useful for future breeding.

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Metabolic mechanisms of Coilia nasus in the natural food intake state during migration

Cited by 13 publications

References 72 publications

Transcriptome Sequencing Analysis of Sex-Related Genes in the Gonads of Mytilus unguiculatus

Transcriptome Sequencing Analysis of Sex-Related Genes in the Gonads of Mytilus unguiculatus

Changes in Serum Fatty Acid Composition and Metabolome-Microbiome Responses of Heigai Pigs Induced by Dietary N-6/n-3 Polyunsaturated Fatty Acid Ratio

A delayed and unsynchronized ovary development as revealed by transcriptome of brain and pituitary of Coilia nasus

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