Metabolic pathways responsible for consumption of aromatic hydrocarbons by microbial associations: Molecular-genetic characterization

Khomenkov, V. G.; Шевелев, А. Б.; Жуков, В. Г.; Kurlovich, A. E.; Загустина, Н. А.; Popov, Vladimir O.

doi:10.1007/s10438-005-0044-2

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…However, the arrangement of degradative genes in sphingomonads is complex (a characteristic also found in Sphingobium sp. AM) with genes scattered across several gene clusters in contrast to the coordinately regulated organized operonic structure of genes in Burkholderia , Pseudomonas and Rhodococcus [ 49 ], where genes coding for the meta-cleavage of aromatic rings are largely located on plasmids including promiscuous plasmids pWWO and pTOL [ 54 ]. In addition, several genes are a part of, or flanked by, transposon or transposon-like sequences, which suggests that PAH degradation genes are able to spread rapidly within bacterial communities by horizontal gene transfer [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

et al. 2017

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The present study describes the behavior of a natural phenanthrene-degrading consortium (CON), a synthetic consortium (constructed with isolated strains from CON) and an isolated strain form CON (Sphingobium sp. AM) in phenanthrene cultures to understand the interactions among the microorganisms present in the natural consortium during phenanthrene degradation as a sole carbon and energy source in liquid cultures. In the contaminant degradation assay, the defined consortium not only achieved a major phenanthrene degradation percentage (> 95%) but also showed a more efficient elimination of the intermediate metabolite. The opposite behavior occurred in the CON culture where the lowest phenanthrene degradation and the highest HNA accumulation were observed, which suggests the presence of positive and also negative interaction in CON. To consider the uncultured bacteria present in CON, a metagenomic library was constructed with total CON DNA. One of the resulting scaffolds (S1P3) was affiliated with the Betaproteobacteria class and resulted in a significant similarity with a genome fragment from Burkholderia sp. HB1 chromosome 1. A complete gene cluster, which is related to one of the lower pathways (meta-cleavage of catechol) involved in PAH degradation (ORF 31–43), mobile genetic elements and associated proteins, was found. These results suggest the presence of at least one other microorganism in CON besides Sphingobium sp. AM, which is capable of degrading PAH through the meta-cleavage pathway. Burkholderiales order was further found, along with Sphingomonadales order, by a metaproteomic approach, which indicated that both orders were metabolically active in CON. Our results show the presence of negative interactions between bacterial populations found in a natural consortium selected by enrichment techniques; moreover, the synthetic syntrophic processing chain with only one microorganism with the capability of degrading phenanthrene was more efficient in contaminant and intermediate metabolite degradation than a generalist strain (Sphingobium sp. AM).

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Section: Discussionmentioning

confidence: 99%

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

et al. 2017

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Literature overview: Microbial metabolism of high molecular weight polycyclic aromatic hydrocarbons

Husain

2008

Remediation Journal

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High molecular weight polycyclic aromatic hydrocarbons (HMW PAHs) increase in hydrophobicity with increases in their molecular weight and ring angularity. Microbial strategies to deal with PAH hydrophobicity include biofilm formation, enzyme induction, and biosurfactants, the effect of which is variable on PAH metabolism depending on the surfactant type and concentration, substrate, and microbial strain(s). Aerobic HMW PAH metabolism proceeds via mineralization, partial degradation, and cometabolic transformations. Generally, bacteria and nonlignolytic fungi metabolize PAHs via initial PAH ring oxidation by dioxygenases to form cis-dihydrodiols, which are transformed to catechol compounds by dehydrogenases and other mono-and dioxygenases to substituted catechol and noncatechol compounds, all ortho-or metacleaved and further oxidized to simpler compounds. However, lignolytic fungi form quinones and acids to CO 2 . This review discusses the pathways for HMW PAH microbial metabolism. O

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Identification of pyrene‐degradation pathways: Bench‐scale studies using Pseudomonas fluorescens 29L

Husain

2008

Remediation Journal

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Metabolic pathways responsible for consumption of aromatic hydrocarbons by microbial associations: Molecular-genetic characterization

Cited by 3 publications

References 10 publications

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

Literature overview: Microbial metabolism of high molecular weight polycyclic aromatic hydrocarbons

Identification of pyrene‐degradation pathways: Bench‐scale studies using Pseudomonas fluorescens 29L

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