Metabolic plasticity of <i>Salmonella enterica</i> as adaptation strategy in river water

González-López, Irvin; Medrano-Félix, José Andrés; Campo, Nohelia Castro‐del; López-Cuevas, Osvaldo; Rodríguez, Juan Ramón Ibarra; Martínez-Rodríguez, Célida Isabel; Valdez-Torres, José Benigno; Cháidez, Cristóbal

doi:10.1080/09603123.2021.1896682

Cited by 3 publications

(1 citation statement)

References 42 publications

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“…Although Salmonella commonly resides in the gastrointestinal tract of animals, this pathogen is highly persistent in aquatic environments due to its ability to prevail for long periods in soils and sediments and to successfully adapt to stressful conditions, such as fluctuating temperature and pH, desiccation, and nutrient deprivation [9][10][11]. In the subtropical environments found in the Culiacan Valley, surface waters such as rivers and streams have been reported as relevant reservoirs for Salmonella due to optimal growth temperatures and the availability of nutrients (glucose, N-acetyl-D-glucosamine and D-glucosamine acid), enabling the prevalence of Salmonella in these aquatic environments [12,13]. Additionally, several studies indicated that organic matter transported into rivers by stormwater runoff promotes an increase in Salmonella populations [14][15][16].…”

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confidence: 99%

Prevalence and Genomic Diversity of Salmonella enterica Recovered from River Water in a Major Agricultural Region in Northwestern Mexico

et al. 2022

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Salmonella enterica is a leading cause of human gastrointestinal disease worldwide. Given that Salmonella is persistent in aquatic environments, this study examined the prevalence, levels and genotypic diversity of Salmonella isolates recovered from major rivers in an important agricultural region in northwestern Mexico. During a 13-month period, a total of 143 river water samples were collected and subjected to size-exclusion ultrafiltration, followed by enrichment, and selective media for Salmonella isolation and quantitation. The recovered Salmonella isolates were examined by next-generation sequencing for genome characterization. Salmonella prevalence in river water was lower in the winter months (0.65 MPN/100 mL) and significantly higher in the summer months (13.98 MPN/100 mL), and a Poisson regression model indicated a negative effect of pH and salinity and a positive effect of river water temperature (p = 0.00) on Salmonella levels. Molecular subtyping revealed Oranienburg, Anatum and Saintpaul were the most predominant Salmonella serovars. Single nucleotide polymorphism (SNP)-based phylogeny revealed that the detected 27 distinct serovars from river water clustered in two major clades. Multiple nonsynonymous SNPs were detected in stiA, sivH, and ratA, genes required for Salmonella fitness and survival, and these findings identified relevant markers to potentially develop improved methods for characterizing this pathogen.

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Section: Introductionmentioning

confidence: 99%

Prevalence and Genomic Diversity of Salmonella enterica Recovered from River Water in a Major Agricultural Region in Northwestern Mexico

et al. 2022

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Genomic characteristics of Salmonella Montevideo and Pomona: impact of isolation source on antibiotic resistance, virulence and metabolic capacity

Garrido-Palazuelos,

Aguirre-Sánchez,

Castro-Del Campo

et al. 2024

International Journal of Environmental Health Research

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Activity of plant essential oils against clinically and environmentally isolatedSalmonella entericaserotypes:in vitroassays and molecular docking

Rochín-Medina

Mendoza-López

Campo

et al. 2023

Letters in Applied Microbiology

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This research aimed to evaluate the antimicrobial activity of essential oils (EOs) against clinically and environmentally-isolated Salmonella serotypes. Oregano, thyme, and grapefruit EO compounds were identified, and the antimicrobial activity was evaluated against the S. Saintpaul, Oranienburg, and Infantis serotypes. In addition, molecular docking was performed to explore the possible mechanisms between compounds of EOs with microbial enzymes. Thymol was the main compound identified in oregano (44.0%) and thyme (31%) EOs, while D-limonene was present in a greater proportion in grapefruit EO. Oregano EO had the highest antimicrobial activity, followed by thyme and grapefruit EOs. Oregano and thyme EOs illustrated a greater inhibitory capacity to all serotypes, particularly with the environmental S. Saintpaul. Oregano EO presented values of MIC and MBC of 0.1 μL/mL for all serotypes, while thyme and grapefruit EOs presented MIC values of 0.1 μL/mL for the clinical serotypes S. Infantis and S. Oranienburg, respectively. Molecular docking analysis showed the optimal binding free energies for thymol and carvacrol with glucokinase, ATP-dependent-6-fructokinase, outer membrane porin C, and topoisomerase IV. Our results indicate that these EOs can inhibit clinically and environmentally isolated Salmonella serotypes and can be used as alternatives for developing natural food preservatives.

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Metabolic plasticity of Salmonella enterica as adaptation strategy in river water

Cited by 3 publications

References 42 publications

Prevalence and Genomic Diversity of Salmonella enterica Recovered from River Water in a Major Agricultural Region in Northwestern Mexico

Prevalence and Genomic Diversity of Salmonella enterica Recovered from River Water in a Major Agricultural Region in Northwestern Mexico

Genomic characteristics of Salmonella Montevideo and Pomona: impact of isolation source on antibiotic resistance, virulence and metabolic capacity

Activity of plant essential oils against clinically and environmentally isolatedSalmonella entericaserotypes:in vitroassays and molecular docking

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