Metabolic polymorphisms and the micronucleus frequency in buccal epithelium of adolescents living in an urban environment

Montero, Regina; Serrano, Luis; Dávila, Víctor M.; Segura, Yayoi; Arrieta, Andrea Barrantes; Fuentes, Raquel Acosta; Abad, Iris; Valencia, Lucía; Sierra, Patricia; Camacho, Rafael

doi:10.1002/em.10186

Cited by 25 publications

(9 citation statements)

References 22 publications

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“…These include scraping with a wood spatula (31,34,48,51,52,60,62,91,92), wood tongue depressor (12,32,43,45,56), cotton swab (42), short-bristle cytobrush (28,29,33,40,44,57,95,96), and toothbrush (95,96).…”

Section: Resultsmentioning

confidence: 99%

Smoking and Smokeless Tobacco-Associated Human Buccal Cell Mutations and Their Association with Oral Cancer—A Review

Proia

Paszkiewicz

Nasca³

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

144

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Reported herein are the results of a structured literature review that was undertaken to (a) determine if human buccal (mouth) cell changes are associated with smoking and smokeless (''chewing'') tobacco, (b) tabulate different buccal cell alterations that have been reported, (c) delineate buccal cell assays that have been used successfully, (d) determine whether buccal cell changes correlate with oral cancer as defined in clinicopathologic investigations, and (e) assess the feasibility of developing a high-throughput buccal cell assay for screening smokers for the early detection of oral cancer. The results of the studies reported herein have established that diverse buccal cell changes are associated with smoking and smokeless tobacco. This review documents also that buccal cells have been collected in a noninvasive manner, and repetitively for serial studies, from different sites of the mouth (e.g., cheek, gum, and tongue) and from normal tissue, preneoplastic lesions (leukoplakia), and malignant tumors. Tobacco-associated genetic mutations and nongenetic changes have been reported; a partial listing includes (a) micronuclei, (b) bacterial adherence, (c) genetic mutations, (d) DNA polymorphisms, (d) carcinogen-DNA adducts, and (e) chromosomal abnormalities. Clinical studies have correlated buccal cell changes with malignant tumors, and some oral oncologists have reported that the buccal cell changes are practical biomarkers. Summarily, the literature has established that buccal cells are useful not only for characterizing the molecular mechanisms underlying tobacco-associated oral cancers but also as exfoliative cells that express diverse changes that offer promise as candidate biomarkers for the early detection of oral cancer. (Cancer Epidemiol Biomarkers Prev 2006;15(6):1061 -77)

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Section: Resultsmentioning

confidence: 99%

Smoking and Smokeless Tobacco-Associated Human Buccal Cell Mutations and Their Association with Oral Cancer—A Review

Proia

Paszkiewicz

Nasca³

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

144

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“…Moreover, the basal level of DNA damage in lymphocytes has been shown to be influenced by lifestyle conditions that can include diet, exercise, hormonal intake, smoking and drinking habits, indoor and outdoor pollution, occupational exposure, etc. [18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

“…Accordingly, PBLs could also be used to assess the degree of DNA damage that is induced by abnormal (low or high) levels of some trace elements and metals, which has been linked in some studies with cancer development. In fact, over 90% of the biomonitoring studies have so far assayed human PBLs because they circulate in the body, and the cellular, nuclear, and metabolic state of the blood cells can reflect the overall extent of body exposure [18][19][20][21][22][23]. There is now strong evidence to indicate that a high level of DNA damage measured in lymphocytes reflects an elevated risk of cancer, although lymphocytes are not the cells that comprise solid tumors [18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

“…In fact, over 90% of the biomonitoring studies have so far assayed human PBLs because they circulate in the body, and the cellular, nuclear, and metabolic state of the blood cells can reflect the overall extent of body exposure [18][19][20][21][22][23]. There is now strong evidence to indicate that a high level of DNA damage measured in lymphocytes reflects an elevated risk of cancer, although lymphocytes are not the cells that comprise solid tumors [18][19][20][21][22][23]. Moreover, the basal level of DNA damage in lymphocytes has been shown to be influenced by lifestyle conditions that can include diet, exercise, hormonal intake, smoking and drinking habits, indoor and outdoor pollution, occupational exposure, etc.…”

Section: Introductionmentioning

confidence: 99%

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Abnormal Blood Levels of Trace Elements and Metals, DNA Damage, and Breast Cancer in the State of Kuwait

Saleh

Behbehani

Asfar

et al. 2010

Biol Trace Elem Res

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This study aims at investigating the blood level of Cu, Zn, Se, and Cd in breast cancer patients and the association between such level and the frequency of micronucleated lymphocytes. Fifty stage I breast cancer patients were recruited for this study at the time of diagnosis and before receiving any treatment or surgery. The control group consisted of 150 normal females matched to the patients for age (± 5 years). The whole blood level of Cu, Zn, Se, and Cd was determined using spectrophotometry. The frequency of micronucleated lymphocytes in the blood was determined using the cytokinesis-block micronucleus assay. The level of Cu, Zn, and Se was significantly lower (p = 0.0006, <0.0001, and <0.0001, respectively) in breast cancer patients, as compared to controls. The level of Cd was significantly (p < 0.0001) higher in the patients, as compared to controls. The frequency of lymphocytes with one micronucleus was significantly (p < 0.0001) higher in the patients, as compared to controls. In breast cancer patients, the frequency of micronucleated lymphocytes showed different associations with different levels of these trace elements. High Cd, low Zn, low Se, and both high and low Cu levels were significantly associated with micronucleus formation in lymphocytes. A similar association was found in the normal control group only in relation to high Se and Cd levels. Breast cancer patients seem to have abnormal levels of Cu, Zn, Se, and Cd, and such abnormality is associated with micronucleus formation in lymphocytes.

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“…Few studies so far have investigated genetic damage in children exposed to air pollution [15][16][17][18][19]. In recent years, studies of MN frequency in the BM cells of children and young adults have shown cytogenetic damage in participants living in areas with high concentrations of particulate matter (PM) or oxidant pollutants [20][21][22][23][24][25]. Recently, Ceretti et al [25] found a higher frequency of MN in the exfoliated buccal cells (EBCs) of pre-school children living in a highly polluted town in the Po Valley in Italy than was observed in a pooled general population of the same age.…”

Section: Introductionmentioning

confidence: 99%

Air pollution biological effects in children living in Lecce (Italy) by Buccal micronucleus cytome assay (the MAPEC_LIFE study)

Donno¹,

Grassi²,

Ceretti³

et al. 2016

Int. J. SDP

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The aim of the MAPEC_LIFE (Monitoring Air Pollution Effects on Children for Supporting Public Health Policy) study is to evaluate the associations between the concentrations of air pollutants and early biological effects in children living in five Italian towns (Brescia, Torino, Lecce, Perugia and Pisa) characterised by varying levels of air pollution. This paper presents the results of micronucleus cytome assays performed on the oral mucosa cells of subjects living in Lecce (Puglia, Italy) and their relationship to factors associated with indoor/outdoor exposure and lifestyles. The study was conducted on 6-8-year-old schoolchildren living in Lecce. The micronucleus cytome assay was performed on exfoliated buccal cells collected from the oral mucosa of children using a soft-bristled toothbrush. Micronuclei were evaluated only in normal differentiated cells. Overall, 43.0% of the samples tested were positive, with an average frequency of 0.28 MN/1000 differentiated cells. Data analysis shows positive associations between the frequency of MN in the children's buccal mucosa cells and obesity, heavy traffic and smoking mothers, while outdoor sports seem to have the opposite effect. These data will be integrated with data from the other cities involved in the MAPEC_LIFE study and could be used to build a model for estimating global genotoxic risk.

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Metabolic polymorphisms and the micronucleus frequency in buccal epithelium of adolescents living in an urban environment

Cited by 25 publications

References 22 publications

Smoking and Smokeless Tobacco-Associated Human Buccal Cell Mutations and Their Association with Oral Cancer—A Review

Smoking and Smokeless Tobacco-Associated Human Buccal Cell Mutations and Their Association with Oral Cancer—A Review

Abnormal Blood Levels of Trace Elements and Metals, DNA Damage, and Breast Cancer in the State of Kuwait

Air pollution biological effects in children living in Lecce (Italy) by Buccal micronucleus cytome assay (the MAPEC_LIFE study)

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