Metabolic pools associated with monoterpene biosynthesis in higher plants

Allen, Keith G.; Banthorpe, D. V.; Charlwood, B. V.; Ekundayo, Olusegun; Mann, John

doi:10.1016/s0031-9422(00)89062-7

Cited by 44 publications

(3 citation statements)

References 52 publications

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“…The presence of this radioactive group in the molecule is a strong indication that IPP isomerase does exist in H . brasiliensis latex, and also proves that 14C-GGOH is not formed by reaction of I4C-IPP with a preformed pool of allylic C,-units, as can occur in certain examples of monoterpene biosynthesis (Allen et al, 1976).…”

Section: Biosynthesis Of Oligoisoprenoids In H Brasiliensis Latexmentioning

confidence: 53%

New aspects of rubber biosynthesis

Archer

Audley

1987

Botanical Journal of the Linnean Society

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New aspects of rubber biosynthesis. Following a review of the site of rubber biosynthesis in Hevea brasiliensis and Parthenium argentalum, evidence is given for the initiation of polyisoprene molecules from (ranMerpenoid precursors including geranylgeranyl pyrophosphate. All franj‐14C‐geranylgeraniol has been isolated from incubations of H. brasiliensis latex serum with 14C‐isopentenyl pyrophosphate. Gel‐filtration chromatography of the serum yields very small rubber particles of high biosynthetic activity, and two proteinaceous fractions. One of these increases the biosynthesis of rubber and may contain the enzyme, isopentenyldiphosphate δ‐isomerase, whilst the other appears to inhibit rubber formation. The nature and molecular weight of the rubber formed in vitro is discussed and a mechanism for the de novo formation of rubber particles is suggested.

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Section: Biosynthesis Of Oligoisoprenoids In H Brasiliensis Latexmentioning

confidence: 53%

New aspects of rubber biosynthesis

Archer

Audley

1987

Botanical Journal of the Linnean Society

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“…Other possible explanations, but less likely according to the authors, could be that DMAPP is derived from another pathway, along with compartmentation. Similarly in Tanacetum vulgare, Pelargonium graveolens and Mentha pulegium 115,121 it was found that in monoterpenes the labels of fed IPP are not uniformly channelled into the two C 5 -parts of the molecule. The portion of the molecule derived from IPP is usually more heavily labelled than that derived from DMAPP.…”

Section: Metabolic Pools Of Ipp and Dmappmentioning

confidence: 98%

“…From the various efforts to purify IPP isomerase it is clear that it is hard to separate from prenyltransferases. For example, Allen et al 115 reported the purification of FPP synthase and even upto the last steps of purification the enzyme was able to accept IPP alone as substrate, i.e. isomerase activity was present.…”

Section: Multienzyme Complexesmentioning

confidence: 99%

Isopentenyl diphosphate isomerase: a core enzyme in isoprenoid biosynthesis. A review of its biochemistry and function

Ramos-Valdivia¹,

Heijden²,

Verpoorte³

1997

Nat. Prod. Rep.

143

124

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A convenient enzymatic preparation of specifically‐labelled geraniol

Banthorpe

Branch

1988

Labelled Comp Radiopharmac

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Geraniol was formed in high (up to 36%) yield when equimolar quantities of isopentenyl and 3,3-dimethylallyl pyrophosphates (1 mmole) together with inhibitors of the isomerase that interconverts them were incubated with a strictly defined cell-free extract from flowerheads of Rosa diZecta cv. Lady Seaton. essentially (>98%) complete position specificity. A simple route is thus available, given the appropriately labelled C5 precursors, for the preparation of geraniol and thence by standard chemical routes of its isomers nerol and linalool and their pyrophosphates with any chosen carbon atom specifically labelled at isotope abundances suitable for biosynthetic studies. Similarly, any desired 2H or 3H labelled geraniol or its relatives could in principle be prepared with both regio-and stereo-specificity.The C5 units were incorporated in toto with

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Metabolic pools associated with monoterpene biosynthesis in higher plants

Cited by 44 publications

References 52 publications

New aspects of rubber biosynthesis

New aspects of rubber biosynthesis

Isopentenyl diphosphate isomerase: a core enzyme in isoprenoid biosynthesis. A review of its biochemistry and function

A convenient enzymatic preparation of specifically‐labelled geraniol

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