Metabolic profile of the brown macroalga Sargassum cymosum (Phaeophyceae, Fucales) under laboratory UV radiation and salinity conditions

Polo, Luz K.; Felix, Marthiellen R. L.; Kreusch, Marianne; Pereira, Débora Tomazi; Costa, Giulia Burle; Simioni, Carmen; Martins, Roberta de Paula; Latini, Alexandra; Floh, Eny S. I.; Chow, Fungyi; Ramlov, Fernanda; Maraschin, Marcelo; Bouzon, Zenilda L.; Schmidt, Éder C.

doi:10.1007/s10811-014-0381-8

Cited by 18 publications

(24 citation statements)

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“…Due to tidal exposure, intertidal macroalgae are constantly exposed to fluctuating environmental stresses such as high temperature, desiccation and high radiation levels (PAR and UV radiation) (Sampath-Wiley et al 2008) that could easily lead to the formation and accumulation of free radicals and reactive species, triggering oxidative stress. Photobiological studies in macroalgae indicate diverse physiological disturbances in response to UV radiation, including alterations in growth and development (Altamirano et al 2003, Gao and Xu 2008, Navarro et al 2016, pigment degradation (Heo and Jeon 2009), dynamic or chronic photoinhibition of photosynthesis (Barufi et al 2011, Ayres-Ostrock and Plastino 2014, Figueroa et al 2014, protein and DNA damage (Buma et al 2001, Kumar et al 2004, decrease in lipid/fatty acid content (Khotimchenko andYakovleva 2005, Liang et al 2006), inhibition of enzyme activity (Lee and Shiu 2009), alterations in polyamines content (Polo et al 2014b), and modifications in cellular organization and ultrastructure (Holzinger and Lütz 2006, Polo et al 2014a, Schmidt et al 2015. As a photoprotective mechanism against UV radiation, especially UVB, macroalgae can increase the production of UV-absorbing compounds such as mycosporine-like amino acids, phenolic compounds and carotenoids, which play protective roles by mitigating the damage caused by the increase in reactive species (Ruhland et al 2007), therefore giving advantages that enable macroalgae to survive in the presence of UV radiation.…”

Section: Introductionmentioning

confidence: 99%

Physiological performance by growth rate, pigment and protein content of the brown seaweed <em>Sargassum filipendula</em> (Ochrophyta: Fucales) induced by moderate UV radiation exposure in the laboratory

Polo

Chow

2020

Sci. Mar.

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UV radiation is a factor affecting the distribution and physiology of photosynthetic organisms in an aquatic ecosystem. Studies with macroalgae indicate diverse biological disturbances in response to UV radiation. This work aimed to study sensitivity of the brown macroalga Sargassum filipendula exposed to UV radiation: PAR (control), PAR+UVA+UVB(++) and PAR+UVA(++)+UVB. Changes in the physiological parameters growth rate, total soluble proteins, photosynthetic pigments and the UV-vis absorbing compounds were analysed after T0, T4, T7 and T10 (days) of UV exposure. Physiological parameters showed little variation between treatments and over time, suggesting that moderate UV radiation doses could regulate resistance responses to re-establish the cellular homoeostasis condition through activation of an antioxidant defence system, such as an overproduction of phenolic compounds. Responses recorded in S. filipendula would be related to acclimation mechanisms against acute UV radiation stress, triggering resistance responses to avoid serious damage to the metabolic machinery, activating control systems to maintain hormesis, and homoeostasis of deleterious actions of reactive species, similar to the phenomenon known as preparation for oxidative stress. Finally, UV-visible absorption spectra showed absorption bands evidencing the presence of mainly UV-absorbing compounds with photoprotective function, such as phlorotannins, flavonoids and carotenoids, which could provide adaptive advantages for organisms exposed to UV radiation.

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Section: Introductionmentioning

confidence: 99%

Physiological performance by growth rate, pigment and protein content of the brown seaweed <em>Sargassum filipendula</em> (Ochrophyta: Fucales) induced by moderate UV radiation exposure in the laboratory

Polo

Chow

2020

Sci. Mar.

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“…Under a combined stress of varying salinity and increased UV radiation the brown algae Sargassum cymosum , like the above‐mentioned red alga Grateloupia doryphora, displayed greater tolerance to salinity than to UV stress (Polo et al. ). Interestingly, decreasing free PUT and SPD levels were reported, compared to the generally reported increase in these PAs.…”

Section: Functions Of Polyaminesmentioning

confidence: 99%

“…This may account for the alga's ability to acclimatize to moderate saline stress (Garc ıa-Jim enez et al 2007) as lipid peroxidation, inhibition of ethylene synthesis and proteolytic attack can be prevented by PAs binding to thylakoid membranes in higher plants (Besford et al 1993). Under a combined stress of varying salinity and increased UV radiation the brown algae Sargassum cymosum, like the abovementioned red alga Grateloupia doryphora, displayed greater tolerance to salinity than to UV stress (Polo et al 2014). Interestingly, decreasing free PUT and SPD levels were reported, compared to the generally reported increase in these PAs.…”

Section: Functions Of Polyaminesmentioning

confidence: 99%

Polyamines in macroalgae: advances and future perspectives

Schweikert

Burritt

2015

Journal of Phycology

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Polyamines (PA) are ubiquitous, small, aliphatic cations found in all living cells. In recent years the importance of these molecules for macroalgae has become evident and a substantial body of knowledge has been accumulated over the last three decades. This review summarizes research on the PAs found in macroalgae, their transport and metabolism, and their biological significance in processes such as cell division, chloroplast development, and reproduction. The involvement of PAs in environmental stress responses in macroalgae is also addressed. The discussion of PAs in this review not only demonstrates that PAs play an important role in physiological processes in macroalgae, but also clearly demonstrates the similarities and differences between PA metabolism in macroalgae and higher plants. Key areas for future research are also discussed.

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“…These secondary metabolites have been analysed in marine plants using a targeted metabolite approach with a RP-HPLC based system coupled with fluorescence detection after derivatization of plant crude extract with either dansylchloride or benzoylchloride [36,123]. In marine plants, the function of PAs has been examined in cell division, maturation of reproductive structures and callus induction [124]; however, their function as stress alleviators against salinity, desiccation stress and metal toxicity has only recently been recognized [34][35][36]123,[125][126][127][128]. For example, a hyposaline shock to Grateloupia turuturu has been shown to accumulate free Put, Spd and Spm together with a significant decrease in transglutaminase activity and an increase in arginine-dependent PA synthesis [125].…”

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confidence: 99%

“…The red seaweed Pyropia cinnamomea compensates for UV stress by increasing PA levels, especially bound-soluble and bound-insoluble PAs via the arginine-dependent PA synthesis pathway [123]. Similarly, the higher ratio of Put/Spd, together with a high level of phenolics in Sargassum cymosum when exposed to UVA + UVB radiation suggests that their binding to phenolic molecules results in stabilising the primary cell wall by cross-linking it to cellular components such as polysaccharide-bound phenols [128]. Further, accumulation of GABA possibly from Put degradation via diamine and/or polyamine oxidase activities (DAO and/or PAO) during hypersalinity stress in E. siliculosus has also been documented (Fig.…”

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confidence: 99%

Metabolomics: an emerging frontier of systems biology in marine macrophytes

et al. 2016

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Metabolomics is a rapidly emerging discipline within functional genomics which is increasingly being applied to 19 understand biochemical phenotypes across a range of biological systems. Metabolomics measures all (or a 20 subset) metabolites in a cell at a specific time point, reflecting a snapshot of all the regulatory events responding 21 to the external environmental conditions. Although metabolomics and system biology approaches have been ap-22 plied to the study of terrestrial plants, few marine macrophytes have been examined using these novel technol-23 ogies. Marine macrophytes (including seaweeds and seagrasses) are marine ecosystem engineers delivering a 24 range of ecologically and economically valuable biological services; however they are under threat from a wide 25 range of anthropogenic stressors, climate variation, invasive species and pathogens. Investigating metabolomic 26 regulation in these organisms is crucial to understand their acclimation, adaptation and defence responses to 27 environmental challenges. This review describes the current analytical tools available to study metabolomics 28 in marine macrophytes, along with their limitations for both targeted and non-targeted workflows. To illustrate 29 recent advances in system biology studies in marine macrophytes, we describe how metabolites are used in 30 chemical defence to deter a broad range of invasive species and pathogens, as well as metabolomic 31 reprogramming leading to acclimation or adaptive strategies to environmental and anthropogenic stresses. 32 Where possible, the mechanistic processes associated with primary and secondary plant metabolism governing 33 cellular homeostasis under extreme environments are discussed. Functional integration of metabolomics with 34 the allied "omics" disciplines of transcriptomics and proteomics, as well as the emerging discipline of "fluxomics" 35 are discussed in the context of developing biological system networks, the identification of unknown gene/pro-36 tein functions and the analysis of metabolic pathways in marine plants exposed to stress. Finally, we provide a 37 comprehensive overview of an in silico plant metabolome database that can be utilized to advance our knowl-38 edge from a system biology approach to marine macrophytes.

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Metabolic profile of the brown macroalga Sargassum cymosum (Phaeophyceae, Fucales) under laboratory UV radiation and salinity conditions

Cited by 18 publications

References 100 publications

Physiological performance by growth rate, pigment and protein content of the brown seaweed <em>Sargassum filipendula</em> (Ochrophyta: Fucales) induced by moderate UV radiation exposure in the laboratory

Physiological performance by growth rate, pigment and protein content of the brown seaweed <em>Sargassum filipendula</em> (Ochrophyta: Fucales) induced by moderate UV radiation exposure in the laboratory

Polyamines in macroalgae: advances and future perspectives

Metabolomics: an emerging frontier of systems biology in marine macrophytes

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