Metabolic profiles of exudates from chronic leg ulcerations

Junka, Adam; Wojtowicz, Wojciech; Ząbek, Adam; Krasowski, Grzegorz; Smutnicka, Danuta; Bakalorz, Barbara; Boruta, A.; Dziadas, Mariusz; Młynarz, Piotr; Sedghizadeh, Parish P.; Bartoszewicz, Marzenna

doi:10.1016/j.jpba.2017.01.018

Cited by 18 publications

(15 citation statements)

References 22 publications

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“…In this novel two part human exploratory study, we have demonstrated the complex temporal metabolome and microbiome profiles of the early phases of acute wound healing. To our knowledge this was the first multi-time point study investigating the dynamic metabolome and microbiome in acute wounds, with only three previous studies to date having explored the metabolic profile of chronic wounds at a single time point [11][12][13]. Here, we were able to statistically demonstrate the relationship between the metabolome and microbiome to acute wound healing processes.…”

Section: Discussionmentioning

confidence: 80%

“…The metabolome of wound healing has been understudied. Only two animal studies [9,10] and three human studies [11][12][13] have investigated the metabolic profile of cutaneous wounds at a single time-point using a metabolomics approach. In addition other "omic" technologies such as genomics have been utilised to assess the progressive changes in gene expression in wound healing over time [14,15].…”

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confidence: 99%

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A microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute wounds of human skin: An exploratory study

Ashrafi

Muhamadali

et al. 2020

PLoS ONE

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Profiling skin microbiome and metabolome has been utilised to gain further insight into wound healing processes. The aims of this multi-part temporal study in 11 volunteers were to analytically profile the dynamic wound tissue and headspace metabolome and sequence microbial communities in acute wound healing at days 0, 7, 14, 21 and 28, and to investigate their relationship to wound healing, using non-invasive quantitative devices. Metabolites were obtained using tissue extraction, sorbent and polydimethylsiloxane patches and analysed using GCMS. PCA of wound tissue metabolome clearly separated time points with 10 metabolites of 346 being involved in separation. Analysis of variance-simultaneous component analysis identified a statistical difference between the wound headspace metabolome, sites (P = 0.0024) and time points (P<0.0001), with 10 out of the 129 metabolites measured involved with this separation between sites and time points. A reciprocal relationship between Staphylococcus spp. and Propionibacterium spp. was observed at day 21 (P<0.05) with a statistical correlation between collagen and Propionibacterium (r = 0.417; P = 0.038) and Staphylococcus (r = -0.434; P = 0.03). Procrustes analysis showed a statistically significant similarity between wound headspace and tissue metabolome with non-invasive wound devices. This exploratory study demonstrates the temporal and dynamic nature of acute wound metabolome and microbiome presenting a novel class of biomarkers that correspond to wound healing, with further confirmatory studies now necessary.

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Section: Discussionmentioning

confidence: 80%

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confidence: 99%

A microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute wounds of human skin: An exploratory study

Ashrafi

Muhamadali

et al. 2020

PLoS ONE

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“…Venn diagram was generated with R by the otu table and group. Taxonomic information is necessary, and “summarize_taxa.py” provides the abundance information which can be visualized by histogram [ 22 ]. Alpha diversity and beta diversity are important for the analysis.…”

Section: Methodsmentioning

confidence: 99%

Microbial Diversity of Chronic Wound and Successful Management of Traditional Chinese Medicine

Guo

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Chronic ulcer, including diabetic ulcer, varicose ulcer, and pressure ulcer, negatively affects patients' quality of life. As microbiology plays an important role in the mechanism of pathology for chronic wound healing, this study concentrates on microecology environment of the wound and how Traditional Chinese Medicine (TCM) regulates wound bacteria. Method. The study took wound samples from 35 patients and analyzed bacteria variation before and after TCM treatment by 16s rRNA sequencing. All samples were evaluated from aspects of α-diversity, β-diversity, and Simpson's Diversity index. Result. After total DNA extraction, PCR, and 16S rRNA sequencing of wound bacteria from 35 individuals, it was discovered that younger patients with shorter course of disease have a higher microbial diversity and were easier to recover from ulcers. Additionally, gender also played a vital role in wound healing, and a significant microbial diversity existed between male and female patients. Conclusion. Patients with chronic ulcers achieved a positive effect after TCM treatment (skin-producing ointment). Mechanistically, TCM helped promote wound healing by regulating the wound microbiota.

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“…Studies of aspirated wound fluid have also shed light on new biomarker discoveries involved in chronic wound healing, including detectable increases in levels of tissue metalloproteinases (MMPs) . Technologies using quantitative assessments of MMPs are currently being tested for use as predictive biomarkers for non‐healing ulcers in clinical trials .…”

Section: Utilization Of Human Samples: Diagnostic Tools and Biomarkermentioning

confidence: 99%

“…The characterization of wound microbiome has revealed unique signatures associated with clinical outcomes of healing . Loesche et al evaluated microbiota colonizing DFU using 16S ribosomal RNA gene amplification to create microbial “footprints” and assess microbial diversity in correlation with DFU healing .…”

Section: Utilization Of Human Samples: Diagnostic Tools and Biomarkermentioning

confidence: 99%

Descriptive vs mechanistic scientific approach to study wound healing and its inhibition: Is there a value of translational research involving human subjects?

Pastar

Wong

Egger

et al. 2018

Experimental Dermatology

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The clinical field of wound healing is challenged by numerous hurdles. Not only are wound-healing disorders complex and multifactorial, but the corresponding patient population is diverse, often elderly and burdened by multiple comorbidities such as diabetes and cardiovascular disease. The care of such patients requires a dedicated, multidisciplinary team of physicians, surgeons, nurses and scientists. In spite of the critical clinical need, it has been over 15 years since a treatment received approval for efficacy by the FDA in the United States. Among the reasons contributing to this lack of effective new treatment modalities is poor understanding of mechanisms that inhibit healing in patients. Additionally, preclinical models do not fully reflect the disease complexity of the human condition, which brings us to a paradox: if we are to use a "mechanistic" approach that favours animal models, we can dissect specific mechanisms using advanced genetic, molecular and cellular technologies, with the caveat that it may not be directly applicable to patients. Traditionally, scientific review panels, for either grant funding or manuscript publication purposes, favour such "mechanistic" approaches whereby human tissue analyses, deemed "descriptive" science, are characterized as a "fishing expedition" and are considered "fatally flawed." However, more emerging evidence supports the notion that the use of human samples provides significant new knowledge regarding the molecular and cellular mechanisms that control wound healing and contribute to inhibition of the process in patients. Here, we discuss the advances, benefits and challenges of translational research in wound healing focusing on human subject research.

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Metabolic profiles of exudates from chronic leg ulcerations

Cited by 18 publications

References 22 publications

A microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute wounds of human skin: An exploratory study

A microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute wounds of human skin: An exploratory study

Microbial Diversity of Chronic Wound and Successful Management of Traditional Chinese Medicine

Descriptive vs mechanistic scientific approach to study wound healing and its inhibition: Is there a value of translational research involving human subjects?

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