Metabolic profiling and gene expression analysis reveal the quality deterioration of postharvest toon buds between two different storage temperatures

Zhao, Huihui; Shen, Cheng; Hao, Qingping; Fan, Mingqin; Liu, Xiaoli; Wang, Juan

doi:10.3389/fpls.2023.1142840

Cited by 2 publications

(1 citation statement)

References 39 publications

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“…It is well-known that the composition and content of chemical components of Chinese herbs change with soil conditions, cultivation measures and climate factors in the growth process [ 21 , 22 , 23 , 24 , 25 ]. In addition, relevant studies have shown that the primary processing, storage time and storage method of TCMs also affect the quality of Chinese herbs [ 26 , 27 , 28 , 29 , 30 ]. This may help to explain why the correlation coefficients for the samples from different origins, storage time and kneading processing in our investigation varied.…”

Section: Resultsmentioning

confidence: 99%

Quality Assessment and Classification of Codonopsis Radix Based on Fingerprints and Chemometrics

et al. 2023

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In China, Codonopsis Radix (CR) is frequently consumed both as food and medicine. Here, a comprehensive strategy based on fingerprinting and chemometric approaches was created to explore the influence of origins, storage time and kneading processing on the quality of CR. Firstly, high-performance liquid chromatography with diode array detection was used to obtain the fingerprints of 35 batches of CR from six different origins and 33 batches of CR from varying storage times or kneading procedures. Secondly, chemometric methods including similarity analysis (SA), principal component analysis (PCA), hierarchical clustering analysis (HCA), and two-way orthogonal partial least square with discriminant analysis (O2PLS—DA) were used to evaluate the differences of chemical components in CR so as to identify its source and reflect its quality. Moreover, 13 and 16 major compounds were identified as marker compounds for the discrimination of CR from different origins, storage time and kneading processing, respectively. Furthermore, the relative content of the marker components and the exact content of Lobetyolin were measured, indicating that the contents of these components vary significantly between various CR samples. Meanwhile, the chemical components of CR were identified using Mass spectrometry. According to the findings of our investigation, the quality of CR from Gansu was the best, followed by Shanxi and then Sichuan. The quality of CR from Chongqing and Guizhou was poor. At the same time, the quality of CR was the best when it was kneaded and stored for 0 years, indicating that the traditional kneading process of CR is of great significance. Conclusively, HPLC fingerprint in conjunction with chemical pattern recognition and component content determination can be employed to differentiate the raw materials of different CR samples. Additionally, it is also a reliable, comprehensive and prospective method for quality control and evaluation of CR.

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Section: Resultsmentioning

confidence: 99%

Quality Assessment and Classification of Codonopsis Radix Based on Fingerprints and Chemometrics

et al. 2023

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Unveiling the molecular dynamics of low temperature preservation in postharvest lotus seeds: a transcriptomic perspective

Chen,

Dong,

Song

et al. 2024

BMC Plant Biol

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Background Postharvest quality deterioration poses a significant challenge to the commercial value of fresh lotus seeds. Low temperature storage is widely employed as the primary method for preserving postharvest lotus seeds during storage and transportation. Results This approach effectively extends the storage life of lotus seeds, resulting in distinct physiological changes compared to room temperature storage, including a notable reduction in starch, protein, H 2 O 2 , and MDA content. Here, we conducted RNA-sequencing to generate global transcriptome profiles of postharvest lotus seeds stored under room or low temperature conditions. Principal component analysis (PCA) revealed that gene expression in postharvest lotus seeds demonstrated less variability during low temperature storage in comparison to room temperature storage. A total of 14,547 differentially expressed genes (DEGs) associated with various biological processes such as starch and sucrose metabolism, energy metabolism, and plant hormone signaling response were identified. Notably, the expression levels of DEGs involved in ABA signaling were significantly suppressed in contrast to room temperature storage. Additionally, nine weighted gene co-expression network analysis (WGCNA)-based gene molecular modules were identified, providing insights into the co-expression relationship of genes during postharvest storage. Conclusion Our findings illuminate transcriptional differences in postharvest lotus seeds between room and low temperature storage, offering crucial insights into the molecular mechanisms of low temperature preservation in lotus seeds. Supplementary Information The online version contains supplementary material available at 10.1186/s12870-024-05468-9.

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Metabolic profiling and gene expression analysis reveal the quality deterioration of postharvest toon buds between two different storage temperatures

Cited by 2 publications

References 39 publications

Quality Assessment and Classification of Codonopsis Radix Based on Fingerprints and Chemometrics

Quality Assessment and Classification of Codonopsis Radix Based on Fingerprints and Chemometrics

Unveiling the molecular dynamics of low temperature preservation in postharvest lotus seeds: a transcriptomic perspective

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