Metabolic Profiling and Molecular Mechanisms Underlying Melatonin-Induced Secondary Metabolism of Postharvest Goji Berry (Lycium barbarum L.)

Wang, Junjie; Zhang, Huaiyu; Hou, Jie; Yang, En; Zhao, Lunaike; Zhou, Yueli; Ma, Wenping; Ma, Danmei; Li, Jiayi

doi:10.3390/foods12234326

Cited by 3 publications

References 38 publications

(89 reference statements)

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Carvacrol maintains antioxidant capacity in goji fruit by increasing the content of bioactive compounds

Wang,

Zhao,

Tian

et al. 2024

LWT

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Carvacrol maintains antioxidant capacity in goji fruit by increasing the content of bioactive compounds

Wang,

Zhao,

Tian

et al. 2024

LWT

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Ethylene Modulates the Phenylpropanoid Pathway by EnhancingVvMYB14Expression via the ERF5-Melatonin-ERF104 Pathway in Grape Seeds

Gao,

Wang,

Wang

et al. 2024

Preprint

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Ethylene plays a crucial role in regulating polyphenol metabolism, however the underlying mechanism remains largely unknown. This work demonstrated that ethylene release occurred earlier than melatonin during seed ripening. Ethylene treatment increased the VvASMT expression and melatonin content. VvERF5 was elucidated to bind to the ERE element in the VvASMT promoter. VvERF5 overexpression increased ASMT expression and melatonin content while its suppression generated the opposite results in grape seeds, calli and/or Arabidopsis seeds. Using the promoter of VvMYB14, which was strongly induced by melatonin, a melatonin responsive element (MTRE) was identified. VvERF104 was revealed not only to be strongly induced by melatonin but to bind to the MTRE of the VvMYB14. VvERF104 overexpression and suppression largely increased and decreased the MYB14 expression, respectively, in grape seeds, calli and/or Arabidopsis seeds. VvMYB14 overexpression widely modified the expression of genes in phenylpropanoid pathway and phenolic compound content in grape seeds. DAP-seq revealed that the MEME-1 motif was the most likely binding sites of VvMYB14. VvPAL, VvC4H and VvCHS were verified to be the target genes of VvMYB14. Additionally, the roles of VvERF5, VvASMT and VvERF104 in mediating ethylene-induced changes in phenylpropanoid pathway were elucidated using their suppressing seeds. Collectively, ethylene increased the VvMYB14 expression via the pathway of ERF5-melatonin-ERF104 and thereby modified phenylpropanoid pathway.

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Melatonin Affects Peucedanum praeruptorum Vegetative Growth and Coumarin Synthesis by Modulating the Antioxidant System, Photosynthesis, and Endogenous Hormones

Wan,

Zhang,

Wang

et al. 2024

Journal of Pineal Research

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The dried root of Peucedanum praeruptorum is often used medicinally and has high pyran‐ and furanocoumarin content. Although exogenous melatonin (MT) impacts the regulation of plant growth, stress responses, secondary metabolism, etc., it remains unclear whether MT regulates the vegetative growth and development of P. praeruptorum. Thus, the aim of the current study is to characterize the effects of different exogenous MT concentrations on the physiological functions, photosynthesis, antioxidant systems, hormone induction, and coumarin synthesis of P. praeruptorum. Different MT concentrations exert distinct regulatory effects on P. praeruptorum growth and the expression of genes related to coumarin synthesis. Treatment of P. praeruptorum with low concentrations of MT increases photosynthesis and leaf growth compared to the control, while high concentrations reduce root vitality and elongation and decrease the expression of photosynthetic system genes. Low concentrations of MT also significantly increase antioxidant enzyme activity and photosynthetic pigment content and modulate the levels of IAA, gibberellic acid, salicylic acid, jasmonic acid, abscisic acid, and endogenous MT. Moreover, MT increases the activity of the MT synthesis enzymes tryptophan decarboxylase, tryptophan hydroxylase, tryptamine‐5‐hydroxylase, serotonin N‐acetyltransferase, acetylserotonin O‐methyltransferase, and caffeic acid O‐methyltransferase, and promotes the accumulation of isoscopoletin, scopoletin, peucedanocoumarin II, praeruptorin A, praeruptorin B, and praeruptorin E. MT also upregulates most genes associated with coumarin synthesis, including PAL1, C4H, 4CL‐3, C3H‐1, F6H‐1, CCoAMT, OMT‐1, CYP71AJ1, CYP84A1‐1, S8H‐1, PT‐1, and COSY‐1. These findings demonstrate that MT may improve P. praeruptorum growth and development while promoting the synthesis of coumarin components.

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Metabolic Profiling and Molecular Mechanisms Underlying Melatonin-Induced Secondary Metabolism of Postharvest Goji Berry (Lycium barbarum L.)

Cited by 3 publications

References 38 publications

Carvacrol maintains antioxidant capacity in goji fruit by increasing the content of bioactive compounds

Carvacrol maintains antioxidant capacity in goji fruit by increasing the content of bioactive compounds

Ethylene Modulates the Phenylpropanoid Pathway by EnhancingVvMYB14Expression via the ERF5-Melatonin-ERF104 Pathway in Grape Seeds

Melatonin Affects Peucedanum praeruptorum Vegetative Growth and Coumarin Synthesis by Modulating the Antioxidant System, Photosynthesis, and Endogenous Hormones

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