Metabolic profiling in Caenorhabditis elegans provides an unbiased approach to investigations of dosage dependent lead toxicity

Sudama, Gita; Zhang, Junyi; Isbister, Jenefir D.; Willett, James D.

doi:10.1007/s11306-012-0438-0

Cited by 10 publications

(6 citation statements)

References 52 publications

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“…It seems that the toxicity of these two metals occurs only during chronic exposures. However, cadmium and lead, different from copper, are redox inactive metals, whose toxic effects are induced mainly by acting on the sulfhydryls groups of the proteins [12,45]. Consequently, it is possible that the concentrations of metals required for Cd 2+ and Pb 2+ to induct this response enzyme are well below those that cause a crisis in the organism or a visible degradation of the marine ecosystem.…”

Section: Exposure Phasementioning

confidence: 99%

Catalase Activity in Brown Mussels (Perna perna) under Acute Cadmium, Lead, and Copper Exposure and Depuration Tests

Boudjema

Kourdali²,

Bounakous³

et al. 2014

Journal of Marine Biology

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Brown mussels (Perna perna) were exposed to cadmium (Cd), lead (Pb), and copper (Cu) concentrations under acute exposure and exposure-depuration tests for the estimation of biochemical biomarker catalase (CAT). The acute tests showed accumulated Cd, Pb, and Cu inPerna pernacorrelated linearly with the exposure concentrations (R2=0.794,R2=0.891, andR2=0.985for Cd, Pb, and Cu, resp.). The results of CAT increased significantly in tissues of treatment mussels after 72 h exposure when compared to control. The values of total protein were disturbed in exposed groups when compared with control. These results suggest that metabolites and catalase activity were affected by heavy metal exposures. Analysis using the Spearman rank correlation coefficient showed that the CAT activity appeared to have a significant positive correlativity (Rs=0.921,Rs=0.949, andRs=0.949for Cd, Pb, and Cu, resp.) with the accumulated Cd, Pb, and Cu concentrations, respectively. The result of exposure-depuration tests showed that there is a general tendency for CAT to decrease in depuration phase, suggesting that the induction of catalase is metal and/or mixture of metals dependent.

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Section: Exposure Phasementioning

confidence: 99%

Catalase Activity in Brown Mussels (Perna perna) under Acute Cadmium, Lead, and Copper Exposure and Depuration Tests

Boudjema

Kourdali²,

Bounakous³

et al. 2014

Journal of Marine Biology

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“…HPLC was used to measure the ADP, ATP, and AMP contents of eggs and juveniles of H. schachtii to calculate the adenylate energy charge and thereby extract information on cyst viability and the efficacy of nematocidal fumigants [ 25 , 26 ]. The intermediate profiles of the tyrosine, tryptophan, and purine pathways in Caenorhabditis elegans were analyzed by HPLC coupled to electrochemical detection to study the effects of lead exposure on nematodes [ 27 ]. The metabolites (ascarosides) that constitute chemical communication in the model nematode C. elegans were characterized by ESI-MS/MS and GC-MS analytical techniques [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolic Analysis of the Development of the Plant-Parasitic Cyst Nematodes Heterodera schachtii and Heterodera trifolii by Capillary Electrophoresis Time-of-Flight Mass Spectrometry

Lee

Kim

Mani

et al. 2021

IJMS

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The cyst nematodes Heterodera schachtii and Heterodera trifolii, whose major hosts are sugar beet and clover, respectively, damage a broad range of plants, resulting in significant economic losses. Nematodes synthesize metabolites for organismal development and social communication. We performed metabolic profiling of H. schachtii and H. trifolii in the egg, juvenile 2 (J2), and female stages. In all, 392 peaks were analyzed by capillary electrophoresis time-of-flight mass spectrometry, which revealed a lot of similarities among metabolomes. Aromatic amino acid metabolism, carbohydrate metabolism, choline metabolism, methionine salvage pathway, glutamate metabolism, urea cycle, glycolysis, gluconeogenesis, coenzyme metabolism, purine metabolism, pyrimidine metabolism, and tricarboxylic acid (TCA) cycle for energy conversion (β-oxidation and branched-chain amino acid metabolism) energy storage were involved in all stages studied. The egg and female stages synthesized higher levels of metabolites compared to the J2 stage. The key metabolites detected were glycerol, guanosine, hydroxyproline, citric acid, phosphorylcholine, and the essential amino acids Phe, Leu, Ser, and Val. Metabolites, such as hydroxyproline, acetylcholine, serotonin, glutathione, and glutathione disulfide, which are associated with growth and reproduction, mobility, and neurotransmission, predominated in the J2 stage. Other metabolites, such as SAM, 3PSer, 3-ureidopropionic acid, CTP, UDP, UTP, 3-hydroxy-3-methylglutaric acid, 2-amino-2-(hydroxymethyl-1,3-propanediol, 2-hydroxy-4-methylvaleric acid, Gly Asp, glucuronic acid-3 + galacturonic acid-3 Ser-Glu, citrulline, and γ-Glu-Asn, were highly detected in the egg stage. Meanwhile, nicotinamide, 3-PG, F6P, Cys, ADP-Ribose, Ru5P, S7P, IMP, DAP, diethanolamine, p-Hydroxybenzoic acid, and γ-Glu-Arg_divalent were unique to the J2 stage. Formiminoglutamic acid, nicotinaminde riboside + XC0089, putrescine, thiamine 2,3-dihydroxybenzoic acid, 3-methyladenine, caffeic acid, ferulic acid, m-hydrobenzoic acid, o- and p-coumaric acid, and shikimic acid were specific to the female stage. Overall, highly similar identities and quantities of metabolites between the corresponding stages of the two species of nematode were observed. Our results will be a valuable resource for further studies of physiological changes related to the development of nematodes and nematode–plant interactions.

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“…They used HPLC with coulometric array detection to detect and quantify metabolites based on their oxidation-reduction potentials. After lead exposure, changes in tryptophan, tyrosine and purine have been observed in WT worms [ 145 ].…”

Section: Applications Of C Elegans Metabolomicsmentioning

confidence: 99%

Quo Vadis Caenorhabditis elegans Metabolomics—A Review of Current Methods and Applications to Explore Metabolism in the Nematode

Salzer

Witting

2021

Metabolites

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Metabolomics and lipidomics recently gained interest in the model organism Caenorhabditis elegans (C. elegans). The fast development, easy cultivation and existing forward and reverse genetic tools make the small nematode an ideal organism for metabolic investigations in development, aging, different disease models, infection, or toxicology research. The conducted type of analysis is strongly depending on the biological question and requires different analytical approaches. Metabolomic analyses in C. elegans have been performed using nuclear magnetic resonance (NMR) spectroscopy, direct infusion mass spectrometry (DI-MS), gas-chromatography mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS) or combinations of them. In this review we provide general information on the employed techniques and their advantages and disadvantages in regard to C. elegans metabolomics. Additionally, we reviewed different fields of application, e.g., longevity, starvation, aging, development or metabolism of secondary metabolites such as ascarosides or maradolipids. We also summarised applied bioinformatic tools that recently have been used for the evaluation of metabolomics or lipidomics data from C. elegans. Lastly, we curated metabolites and lipids from the reviewed literature, enabling a prototypic collection which serves as basis for a future C. elegans specific metabolome database.

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Metabolic profiling in Caenorhabditis elegans provides an unbiased approach to investigations of dosage dependent lead toxicity

Cited by 10 publications

References 52 publications

Catalase Activity in Brown Mussels (Perna perna) under Acute Cadmium, Lead, and Copper Exposure and Depuration Tests

Catalase Activity in Brown Mussels (Perna perna) under Acute Cadmium, Lead, and Copper Exposure and Depuration Tests

Metabolic Analysis of the Development of the Plant-Parasitic Cyst Nematodes Heterodera schachtii and Heterodera trifolii by Capillary Electrophoresis Time-of-Flight Mass Spectrometry

Quo Vadis Caenorhabditis elegans Metabolomics—A Review of Current Methods and Applications to Explore Metabolism in the Nematode

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