Metabolic Profiling in Ruminant Diagnostics

Saun, Robert J. Van

doi:10.1016/j.cvfa.2022.10.004

Cited by 6 publications

(1 citation statement)

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“…A comprehensive metabolic profile includes parameters measured in the blood in order to determine energy metabolism (glucose, nonesterified fatty acids—NEFA, and beta-hydroxybutyrate—BHB), protein metabolism (total protein, albumin, and urea), mineral metabolism (Ca, P, and Mg), functional status of hepatocytes (aspartate aminotrasferase—AST, gamma-glutamyl transferase—GGT, alkaline phosphatase—ALP, total bilirubin—T.Bil, cholesterol, and triglycerides), and hormonal profile related to metabolism (insulin, cortisol, triiodothyronine—T3, and thyroxine—T4) [ 7 , 8 ]. Modern veterinary medicine recommends routine testing of the metabolic profile during the four seasons and in cows in different periods of lactation, as well as when dietary or certain conditional changes occur on the farm or when a disease is suspected.…”

Section: Introductionmentioning

confidence: 99%

Analytical and Clinical Interference of Sample Hemolysis in Evaluating Blood Biochemical and Endocrine Parameters in Cows

Kovačević,

Cincović,

Majkić

et al. 2024

Animals

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Hemolysis is a common cause of errors in laboratory tests as it affects blood parameters and leads to a positive or negative bias. This study aims to examine the relationship between the level of hemolysis (expressed as cell-free hemoglobin concentration, g/L) and the variability of metabolic and endocrine parameters and to determine the threshold level of hemolysis that causes an analytically and clinically significant bias for the twenty most frequently examined blood parameters in cows. Paired blood samples of 10 mL each were obtained from 30 cows. One was subjected to mechanical trauma and plasma was extracted directly from the other. Hemolyzed and non-hemolyzed samples from the same animal were mixed to obtain final samples with cell-free hemoglobin concentrations of 0, 1, 2, 4, 6, 8, and 10 g/L. Metabolic and endocrine parameters were measured in the samples and their deviation and the linear equation between the level of hemolysis and the deviation were determined. The following threshold values of hemolysis were determined, which correspond to the acceptable analytical (lower value) and clinical (upper value) levels of parameter variability: BHB 0.96 and 4.81; NEFA 0.39 and 3.31; GLU 0.38 and 3.90; ALB 1.12 and 6.11; TPROT 1.40 and 6.80; UREA 6.62 and 20.1; TBIL 0.75 and 5.65; AST 0.11 and 2.18; GGT 1.71 and 8.90, LDH 0.01 and 0.11, ALP 0.97 and 2.95; TGC 1.56 and 15.5; CHOL 1.29 and 8.56; Ca 5.68 and 25.7; P 0.57 and 8.43; Mg 1.10 and 8.47; INS 1.15 and 3.89; T3 8.19 and 15.6; T4 8.97 and 18.5; and CORT 2.78 and 11.22 g/L cell-free hemoglobin. Three decision levels are available for each metabolic and endocrine parameter: if hemolysis is below the lower (analytical) threshold value, results can be reported without restriction; if hemolysis is between the lower and upper thresholds, the results can be issued with guidance in the form of corrective linear equations; and if hemolysis is above the upper (clinical) threshold, the results and sample must be discarded. This method contributes to an optimal approach to hemolysis interference with metabolic profile parameters in blood samples from cows.

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Section: Introductionmentioning

confidence: 99%