Metabolic profiling of Rhodiola rosea rhizomes by ¹H NMR spectroscopy

Abstract

This study demonstrated the usefulness of the established procedure for multivariate non-target ¹H NMR metabolic profiling of Rhodiola rosea.

Potential of the Endophytic Fungus Phialocephala fortinii Rac56 Found in Rhodiola Plants to Produce Salidroside and p-Tyrosol

“…The aromatic region (δH 6.5-7.2 ppm, H3/5 and H2/6 proton signals for SAL and TYR were 6.982 ppm to 7.009 ppm) and the sugar region (δH 3-4.5 ppm, H −1 proton signal for SAL was 4.218 ppm to 4.248 ppm) were found to contain aromatic compound and glycosidic constituent peaks identical to those found in commercially available SAL and TYR, respectively ( Figure 4). These findings are also consistent with previous studies [4,21] and lend further support to the authenticity of SAL and TYR produced by the Rac56 strain. Under a microscope, the mycelia were observed to be dark with a size range of 1.5-4.5 µm.…”

“…Chemical shifts were reported as δ values relative to tetramethylsilane used as an internal reference. Spectra related compounds were assigned by comparing fungal extracts and authentic SAL and TYR in CD 3 OD following the method in reference [4,21].…”

Metabolomics as a Bioanalytical Tool for Characterization of Medicinal Plants and Their Phytomedical Preparations

“…Factors affecting metabolite production in medicinal plants are described in Figure 20.3 . Because of the commercial utility, some medicinal plants, such as Rhodiola rosea , a folk medicine in Scandinavia and Russia [46] , are now cultivated at many locations with different geographies. These cultivation s show variations in the metabolite profi le s, which are affected by geographic and soil conditions.…”

“…All relevant data for internal use or for declaration to the authorities can be systematically compared and stored. As an example, using 1 H NMR coupled with PCA, an unsupervised metabolic profi ling has been conducted to discriminate nine rhizomes of R. rosea collected from Switzerland, Russia, and Finland [46] . According to PCA models, volatile compounds characterizing the essential oil spectra have been identifi ed as the discriminating factor of rhizomes of R. rosea [46] .…”

Rhodiola rosea L.: from golden root to green cell factories

“…To date, HPLC-, GC-MS-and NMR-based metabolomics have identified the structures of approximately 140 compounds which belong to different natural product classes Joset et al 2011). Most of its pharmaceutically active secondary metabolites (table 1) are mainly stored in the rhizomes and roots.…”