Metabolic profiling of normal and hypertensive rat kidney tissues by hrMAS-NMR spectroscopy

Abstract

Intact kidney tissue samples of normal and spontaneously hypertensive rats (SHRs) were analyzed by hrMAS-NMR spectroscopy and principal component analysis (PCA). Radial components (cortex, outer stripe of the outer medulla, inner stripe of the outer medulla, and papilla) were sampled from various regions across the kidney from multiple animals in order to establish inter- and intra-animal variability. The effects of temperature were also measured. Papilla was differentiated from the other tissue types, and this variation by tissue type was greater than the effect of temperature on the samples (spectra were compared from samples at 2 and 30 degrees C). This study also revealed long term stability issues of tissue storage at -80 degrees C. The PCA showed that the greatest differentiation between normal rats and SHRs was found in the cortex and the regions in the NMR spectra that were correlated with this variation were identified.

An optimized procedure for metabonomic analysis of rat liver tissue using gas chromatography/time-of-flight mass spectrometry

“…Therefore, tissue-targeted technology has considerable value since many diseases may cause detectable disturbances only to the local metabolic pathways within specific organs [11]. Tissue-targeted metabonomic analyses have been carried out on brain tissue [11][12][13], liver tissue [14][15][16], and kidney tissue [17][18][19]. Metabolite extraction is a critical step in tissue metabonomic study, and the chloroform-methanol-water solvent system is considered the preferred method in several reports [20][21][22][23].…”

GC-MS with ethyl chloroformate derivatization for comprehensive analysis of metabolites in serum and its application to human uremia

“…The footstone of metabonomics is to reliably measure as many metabolites as possible in biological samples, including body fluids (e.g., urine, serum, amniotic fluid, cerebrospinal fluid), single cells and tissues of given organisms or plants, using modern highthroughput analytical instruments. The main analytical techniques usually involve nuclear magnetic resonance (NMR) [5][6][7][8], liquid chromatography-mass spectrometry (LC-MS and LC-MS-MS) [9][10][11], capillary electrophoresis-mass spectrometry (CE-MS) [12,13], and gas chromatography-mass spectrometry (GC-MS) [14,15]. GC-MS has long been used for metabolic profiling study due to its high sensitivity, reliability and the ease of metabolite identification.…”

Magnetic Resonance Spectroscopy (MRS) in Kidney Transplantation: Interest and Perspectives

“…They established the 1 H (hydrogen-1) and 13 C (carbon-13) chemical shift assignments of metabolites found in cortex and/or papilla. Still with 1 H hrmas NMR spectroscopy, a team realised the metabolic profiling of normal and hypertensive rat kidney (Huhn et al, 2004). They showed that better differentiation between both groups occurred in the kidney cortex.…”