Metabolic profiling of Ochradenus baccatus Delile. utilizing UHPLC-HRESIMS in relation to the in vitro biological investigations

Pecio, Łukasz; Kozachok, Solomiia; Saber, Fatema R.; García-Martí, María; El‐Amier, Yasser A.; Mahrous, Engy A.; Świątek, Łukasz; Boguszewska, Anastazja; Skiba, Adrianna; Elosaily, Ahmed H.; Skalicka-Woźniak, Krystyna; Simal‐Gándara, Jesús

doi:10.1016/j.foodchem.2023.135587

Cited by 4 publications

(1 citation statement)

References 45 publications

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“…The cytotoxicity was tested using the microculture tetrazolium assay as previously described [ 38 ]. Briefly, cell monolayers in 96-well plates were treated for 24 h with serial dilutions of test extracts.…”

Section: Methodsmentioning

confidence: 99%

Betaxanthin Profiling in Relation to the Biological Activities of Red and Yellow Beta vulgaris L. Extracts

et al. 2023

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Beta vulgaris L. is an edible plant with health-beneficial activities. The profile of betaxanthins is more complex than previously described in beetroot cultivars. Twenty-four betaxanthins were detected in extracts of the peel and flesh of five cultivars by HPLC-DAD-ESI-MS, of which two new betaxanthins (arginine-Bx and ornithine-Bx) were detected for the first time in B. vulgaris cultivars. The content of betaxanthins in the studied cultivars decreased in the Tytus > Ceryl > Chrobry > Forono > Boldor sequence. The highest content of compounds (1231 mg/100 g DE) was observed in the Tytus cultivar (peel). The peel of B. vulgaris, which is often considered a waste, appeared to be a richer source of betaxanthins compared to its flesh. Antibacterial and antifungal activities were determined against twenty-three microorganisms. Tytus (peel) showed a moderate or good bactericidal effect, especially against the majority of Gram-positive bacteria as well as against most of the tested fungi (MIC = 0.125–0.5 mg/mL) and additionally characterized by low cytotoxicity towards non-cancerous cells (CC50 = 405 μg/mL, CC50—50% cytotoxic concentration). Tytus flesh also showed a high cytotoxicity value against human cervical adenocarcinoma (HeLa), with CC50 of 282 μg/mL. Correlation analysis was used to determine the relationship between the betaxanthin profiles and antimicrobial and anticancer activities. Arginine-Bx, proline-Bx, and tryptophan-Bx were indicated as active against HeLa and the colon cancer cell line (RKO), while asparagine-Bx and phenylalanine-Bx was responsible for activity against all tested bacterial and yeast species. The significant effectiveness and safety of these beetroots make indicated compounds promising applicants as antimicrobial and anticancer agents.

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Section: Methodsmentioning

confidence: 99%

Betaxanthin Profiling in Relation to the Biological Activities of Red and Yellow Beta vulgaris L. Extracts

et al. 2023

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Phytochemical analysis and wound healing properties of Malva parviflora L. ethanolic extract

Anwar,

El Gedaily,

Salama

et al. 2025

Journal of Ethnopharmacology

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Optimizing Green Extraction Methods for Maximizing the Biological Potential of Dandelion, Milk Thistle, and Chamomile Seed Extracts

Milovanovic,

Tyśkiewicz,

Konkol

et al. 2024

Foods

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This study investigates the underutilized potential of agri-crops from the Asteraceae family by employing sustainable and green technologies (supercritical fluid, ultrasound, and Soxhlet extractions) to enhance the recovery of bioactive compounds. A total of 21 extracts from native and waste seeds of dandelion, milk thistle, and chamomile were systematically compared utilizing a combination of solvents (supercritical CO2 and absolute or aqueous ethanol). Supercritical CO2 extraction yielded up to 281 mg/g of oils from native seeds, while conventional techniques with ethanol recovered an additional 142 mg/g of extracts from waste seeds. Notably, waste seed extracts exhibited superior biological activity, including potent antioxidant properties (IC50 values as low as 0.3 mg/mL in the DPPH assay) and broad-spectrum antimicrobial activity against 32 microbial strains, including methicillin-resistant Staphylococcus aureus, Gram-negative bacteria, and yeast strains. Phenolic compounds were abundant, with up to 2126 mg GAE/g, alongside 25.9 mg QE/g flavonoids, and 805.5 mg/kg chlorophyll A. A selective anticancer activity of waste milk thistle extracts was observed, with a selectivity index of 1.9 to 2.7. The oils recovered from native seeds demonstrated lower bioactivity and are well-suited for applications in food. The potent bioactivity of the smaller quantities of waste seed extracts positions them as valuable candidates for pharmaceutical use.

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Metabolic profiling of Ochradenus baccatus Delile. utilizing UHPLC-HRESIMS in relation to the in vitro biological investigations

Cited by 4 publications

References 45 publications

Betaxanthin Profiling in Relation to the Biological Activities of Red and Yellow Beta vulgaris L. Extracts

Betaxanthin Profiling in Relation to the Biological Activities of Red and Yellow Beta vulgaris L. Extracts

Phytochemical analysis and wound healing properties of Malva parviflora L. ethanolic extract

Optimizing Green Extraction Methods for Maximizing the Biological Potential of Dandelion, Milk Thistle, and Chamomile Seed Extracts

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