Metabolic profiling of Piper species by direct analysis using real time mass spectrometry combined with principal component analysis

Chandra, Preeti; Bajpai, Vikas; Srivastva, Mukesh; Kumar, Kamlesh; Kumar, Brijesh

doi:10.1039/c4ay00246f

Cited by 22 publications

(10 citation statements)

References 41 publications

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“…We used the extract of the fruit in this study, since it is the usual part used medicinally and can be harvested sustainably. Although the fruit contains related piperamides, it does not contain piperlongumine, which is mainly present in the root of this plant [32] . The other piperamides present in the fruit such as dihydropiperlongumine likely have similar bioactivity.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Efficacy & Biochemical Mechanism of Cell Death Induction by Piper longum Extract Selectively in In-Vitro and In-Vivo Models of Human Cancer Cells

Ovadje

Tremblay

et al. 2014

PLoS ONE

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BackgroundCurrently chemotherapy is limited mostly to genotoxic drugs that are associated with severe side effects due to non-selective targeting of normal tissue. Natural products play a significant role in the development of most chemotherapeutic agents, with 74.8% of all available chemotherapy being derived from natural products.ObjectiveTo scientifically assess and validate the anticancer potential of an ethanolic extract of the fruit of the Long pepper (PLX), a plant of the piperaceae family that has been used in traditional medicine, especially Ayurveda and investigate the anticancer mechanism of action of PLX against cancer cells.Materials & MethodsFollowing treatment with ethanolic long pepper extract, cell viability was assessed using a water-soluble tetrazolium salt; apoptosis induction was observed following nuclear staining by Hoechst, binding of annexin V to the externalized phosphatidyl serine and phase contrast microscopy. Image-based cytometry was used to detect the effect of long pepper extract on the production of reactive oxygen species and the dissipation of the mitochondrial membrane potential following Tetramethylrhodamine or 5,5,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride staining (JC-1). Assessment of PLX in-vivo was carried out using Balb/C mice (toxicity) and CD-1 nu/nu immunocompromised mice (efficacy). HPLC analysis enabled detection of some primary compounds present within our long pepper extract.ResultsOur results indicated that an ethanolic long pepper extract selectively induces caspase-independent apoptosis in cancer cells, without affecting non-cancerous cells, by targeting the mitochondria, leading to dissipation of the mitochondrial membrane potential and increase in ROS production. Release of the AIF and endonuclease G from isolated mitochondria confirms the mitochondria as a potential target of long pepper. The efficacy of PLX in in-vivo studies indicates that oral administration is able to halt the growth of colon cancer tumors in immunocompromised mice, with no associated toxicity. These results demonstrate the potentially safe and non-toxic alternative that is long pepper extract for cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Efficacy & Biochemical Mechanism of Cell Death Induction by Piper longum Extract Selectively in In-Vitro and In-Vivo Models of Human Cancer Cells

Ovadje

Tremblay

et al. 2014

PLoS ONE

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“…This development was driven in particular by the fact that some cyclobutanoid dimers [5][6][7][8][9][10] , such as innovanoside A, showed high biological activity. Furthermore, numerous cyclobutanoids of superior biological activity were isolated from the Piper genus, such as dipiperamides A-E [11][12][13][14][15][16][17][18] and piperarborenines A-E [19][20][21][22][23][24][25][26][27][28] (Scheme 1) and several total syntheses have been developed. However, there are only a limited number of synthetic approaches to these strained semi-dimers and homo-dimers, such as cross-coupling, re-arrangement, C-H-functionalization and cycloaddition reactions.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of sinapine and its unprecedented ruthenium-catalyzed [2+2] photodimerization

et al. 2019

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“…Chandra et al . used DART‐MS with PCA to differentiate Piper nigrum , the botanical source of black pepper, from other species in the Piper genus . Like the study on cinnamon performed by Avula and co‐workers, this study used PCA to observe clusters corresponding to different species but did not construct any supervised models for classification.…”

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confidence: 99%

Chemometric brand differentiation of commercial spices using direct analysis in real time mass spectrometry

Pavlovich

Dunn

Hall

2016

Rapid Commun. Mass Spectrom.

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Metabolic profiling of Piper species by direct analysis using real time mass spectrometry combined with principal component analysis

Cited by 22 publications

References 41 publications

Evaluation of the Efficacy & Biochemical Mechanism of Cell Death Induction by Piper longum Extract Selectively in In-Vitro and In-Vivo Models of Human Cancer Cells

Evaluation of the Efficacy & Biochemical Mechanism of Cell Death Induction by Piper longum Extract Selectively in In-Vitro and In-Vivo Models of Human Cancer Cells

Synthesis of sinapine and its unprecedented ruthenium-catalyzed [2+2] photodimerization

Chemometric brand differentiation of commercial spices using direct analysis in real time mass spectrometry

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