Metabolic Profiling of Resistant and Susceptible Tobaccos Response Incited by Ralstonia pseudosolanacearum Causing Bacterial Wilt

Abstract

The causal agent of bacterial wilt, Ralstonia pseudosolanacearum, can cause significant economic losses during tobacco production. Metabolic analyses are a useful tool for the comprehensive identification of plant defense response metabolites. In this study, a gas chromatography-mass spectrometry (GC-MS) approach was used to identify metabolites differences in tobacco xylem sap in response to R. pseudosolanacearum CQPS-1 in two tobacco cultivars: Yunyan87 (susceptible to R. pseudosolanacearum) and K326 (quantitatively resistant). Metabolite profiling 7 days post inoculation with R. pseudosolanacearum identified 88 known compounds, 42 of them enriched and 6 depleted in the susceptible cultivar Yunyan87, while almost no changes occurred in quantitatively resistant cultivar K326. Putrescine was the most enriched compound (12-fold) in infected susceptible tobacco xylem, followed by methyl-alpha-d-glucopyranoside (9-fold) and arabinitol (6-fold). Other sugars, amino acids, and organic acids were also enriched upon infection. Collectively, these metabolites can promote R. pseudosolanacearum growth, as shown by the increased growth of bacterial cultures supplemented with xylem sap from infected tobacco plants. Comparison with previous metabolic data showed that beta-alanine, phenylalanine, and leucine were enriched during bacterial wilt in both tobacco and tomato xylem.

Three amino acid residues are required for the recognition of Ralstonia solanacearum RipTPS in Nicotiana tabacum

“…RipTPS G acts as a host-specificity avirulence factor R. solanacearum is an aggressive pathogen with a large repertoire of T3Es (Genin and Denny, 2012). Functional characterization of these effectors is critical for understanding the mechanisms of host specificity and pathogenicity in these economically important pathogens (Kim et al, 2021;Yang et al, 2022). However, to date, only few R. solanacearum T3Es were characterized for their roles in interaction between R. solanacearum and its host/nonhost plants.…”

Induced defense strategies of plants against Ralstonia solanacearum

“…It has also been shown that root extracts of R. solanacearum-susceptible tomato varieties contain various fatty acid derivatives, while the opposite is true in resistant tomato varieties (Zeiss et al, 2018). In tobacco, methyl-alpha-D-glucopyranoside and arabinitol were significantly higher in susceptible tobacco varieties inoculated with R. solanacearum than in resistant varieties (Yang et al, 2022). Such metabolic markers may be more conducive to the colonization and growth of R. solanacearum.…”

“…In tobacco, methyl-alpha-D-glucopyranoside and arabinitol were significantly higher in susceptible tobacco varieties inoculated with R . solanacearum than in resistant varieties ( Yang et al, 2022 ). Such metabolic markers may be more conducive to the colonization and growth of R .…”

Tubulavírus: evolução, taxonomia e interação com seu hospedeiro

“…Para a obtenção da seiva do xilema, plantas de tomate foram crescidas durante 40 dias. Após esse período, os caules das plantas de tomates foram cortados em segmentos de 5 cm e a seiva do xilema foi extraída por centrifugação a 4.000 x g durante 10 minutos a 4ºC, seguido de esterilização por filtração usando filtro 0,22 µm (Millipore Merck) (Metzner et al, 2010;Yang et al, 2022). Em cada poços da placa de 96 poços de poliestireno foram adicionados 198 µL de seiva de xilema e 2 µL de cultura dos isolados com OD600 = 0,1; em seguida as placas foram incubadas a 28 °C por 24 horas sem agitação.…”