Metabolic profiling of seminal plasma from teratozoospermia patients

Mehrparvar, Bahareh; Chashmniam, Saeed; Nobakht, Fatemeh; Amini, Mehdi; Javidi, Maryam; Minai-Tehrani, Arash; Arjmand, Babak; Gilany, Kambiz

doi:10.1016/j.jpba.2019.112903

Cited by 22 publications

(27 citation statements)

References 15 publications

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“…Among these amino acids, glutamate is the most abundant in the seminal plasma, and its concentration is correlated with the sperm concentration, 31 whereas aspartate is correlated with the number and motility of the sperm 16 . Both these amino acids have been repeatedly shown to be decreased in men with male factor infertility 7,14,16,42 . The decrease in only six out of all the amino acids analyzed constitutes an argument in favor of a specific deregulated metabolic or signaling pathway, instead of a proteostasis disturbance.…”

Section: Discussionmentioning

confidence: 99%

“…16 Both these amino acids have been repeatedly shown to be decreased in men with male factor infertility. 7,14,16,42 The decrease in only six out of all the amino acids analyzed constitutes an argument in favor of a specific deregulated metabolic or signaling pathway, instead of a proteostasis disturbance.…”

Section: Discussionmentioning

confidence: 99%

“…It has recently been used in the analysis of the seminal fluid in men with male factor infertility. However, there is a large heterogeneity in the methods used, the populations included, and the results reported, and no clear conclusion has been made so far 7‐16 . Moreover, the majority of these studies used the World Health Organization (WHO) reference values for sperm characteristics 17 .…”

Section: Introductionmentioning

confidence: 99%

“…However, there is a large heterogeneity in the methods used, the populations included, and the results reported, and no clear conclusion has been made so far. [7][8][9][10][11][12][13][14][15][16] Moreover, the majority of these studies used the World Health Organization (WHO) reference values for sperm characteristics. 17 The WHO classification has important clinical implications, but has some limits, in particular its low predictive value for spontaneous pregnancies.…”

Section: Introductionmentioning

confidence: 99%

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Metabolomic signature of the seminal plasma in men with severe oligoasthenospermia

et al. 2020

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Background Male factor is incriminated in approximately 50% of cases of infertility. The metabolomic approach has recently been used in the assessment of sperm quality and male fertility. Materials and methods We analyzed the metabolomic signatures of the seminal plasma in 20 men with severe oligoasthenospermia (prewash total motile sperm count < 5.106) (SOA) and compared it to 20 men with normal semen parameters, with a standardized approach of targeted and quantitative metabolomics using high‐performance liquid chromatography, coupled with tandem mass spectrometry, and the Biocrates Absolute IDQ p180 kit. Results Among the 188 metabolites analyzed, 110 were accurately measured in the seminal plasma. A robust model discriminating the two populations (Q2(cum) = 55.2%) was obtained by OPLS‐DA (orthogonal partial least‐squares discriminant analysis), based on the drop in concentrations of 37 metabolites with a VIP (variable important for projection) greater than 1. Overall, in men with SOA, there was a significant decrease in: 17 phosphatidylcholines and four sphingomyelins; acylcarnitines, with free L‐carnitine being the most discriminating metabolite; polyunsaturated fatty acids; six amino acids (glutamate, aspartate, methionine, tryptophan, proline, and alanine); and four biogenic amines (spermine, spermidine, serotonin, and alpha‐aminoadipate). Discussion Our signature includes several metabolic changes with different impacts on the sperm quality: a change in phospholipid composition and the saturation of their fatty acids that is potentially linked to the deterioration of sperm membranes; a carnitine deficiency that can negatively impact the energy production via fatty acid oxidation and oxidative phosphorylation; and a decreased level of amino acids and biogenic amines that can lead to dysregulated metabolic and signaling pathways. Conclusion We provide a global overview of the metabolic defects contributing to the structural and functional alteration of spermatozoa in severe oligoasthenospermia. These findings offer new insights into the pathophysiology of male factor infertility that could help to develop future specific treatments.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolomic signature of the seminal plasma in men with severe oligoasthenospermia

et al. 2020

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“…Reduced levels of metabolites, such as amino acids, lactate, citrate, creatinine, α‐ketoglutaric acid, spermine and putrescine, were noticed in the seminal plasma of oligoasthenozoospermic patients (Mumcu, Karaer, Dogan, & Tuncay, 2019). On the other hand, amino acids, citric acid, choline, D‐glucose, myo‐inositol, lactate and pyruvate were significantly overexpressed in infertile teratozoospermic patients (Mehrparvar et al., 2020). In oligozoospermic patients, 1 H‐NMR detected altered levels of fructose, myo‐inositol, aspartate and choline, involved in the sperm maturation, energy metabolism and cell membrane composition (Murgia et al., 2020).…”

Section: Metabolomics and Male Reproductionmentioning

confidence: 99%

Proteomics and metabolomics — Current and future perspectives in clinical andrology

et al. 2020

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Proteomics and metabolomics are emerging as promising tools to investigate the molecular mechanisms associated with male infertility. Proteins and metabolites play a pivotal role in regulating the molecular pathways associated with physiological functions of spermatozoa. Semen analysis, physical examination and laboratory work up cannot identify the etiology of infertility in 30%‐40% of cases, which are classified as idiopathic. Therefore, the application of proteomics and metabolomics in the field of andrology will aid to overcome the limitations of the standard semen analysis. Understanding the molecular pathways associated with male infertility will help in planning ad hoc treatments, contributing to the clinical management of infertile patients. In this review, proteomics and metabolomics studies on spermatozoa and seminal plasma are discussed with a focus on molecular biomarkers associated with male infertility‐related conditions.

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