Metabolic Profiling of the Oil of Sesame of the Egyptian Cultivar ‘Giza 32’ Employing LC-MS and Tandem MS-Based Untargeted Method

Mekky, Reham Hassan; Abdel‐Sattar, Essam; Segura‐Carretero, Antonio; Contreras, María del Mar

doi:10.3390/foods10020298

Cited by 23 publications

(4 citation statements)

References 45 publications

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“…The fatty acid (FAs) profile is an important marker of the nutrition quality of oil; sesame seed oil belongs to the oleic–linoleic acid group [ 58 ]. The fatty acid profiles of sesame seeds roasted with different methods are shown in Table 2 .…”

Section: Resultsmentioning

confidence: 99%

Characterizing the Bioactive Ingredients in Sesame Oil Affected by Multiple Roasting Methods

El-Beltagi

Maraei

El-Ansary

et al. 2022

Foods

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Roasting is an important step in sesame (Sesamum indicum L.) processing. The current research was undertaken to evaluate the oil content, fatty acid (FA) profiles, andphysicochemical characteristics of oil recovered from sesame roasted by different methods (cooker oven, stovetop pan, microwave, and electric frying pan). Roasting sesame seeds changed their oil content according to the roasting method used, with content ranging from 49.83% in control to 59.85% in the roasting by microwave. In oils recovered from raw or roasted seeds, seven fatty acids were obtained through gas chromatography. Changes in the fatty acid profiles occurred in all the treatments, and the total unsaturated fatty acid content was higher than that of saturated fatty acids. The obtained peroxide number of sesame oils was inside the rate of 3.90 meq/kg oil for microwave treatment versus 1.59 meq/kg oil for unroasted. The highest acid value was with the stovetop pan treatment at 3.78 mg/g, followed by the microwave treatment at 3.24 mg/g; the oven treatment gave the lowest value at 1.66 mg/g. The lowest iodine value was observed with the electric frying pan treatment (102.30/100g oil), and phytosterols were most abundant with the microwave treatment. Moreover, the phenolic and flavonoid contents and antioxidant activity were the highest with the microwave roasting. The FTIR spectrum illustrated slight differences in peaks intensity (1738, 1454, 1151, 710 cm−1) between the roasting methods used. The finding of the current investigation of roasting methodswas that the fatty acid profiles wereacrossmethods. As is clear from the obtained results, the microwave roasting treatment is the favoured roasting methodfor the healthiest sesame seed oil contents. Sesame seeds are considered a significant and abundant resource with numerous beneficial nutrients that positively affect human health.

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Section: Resultsmentioning

confidence: 99%

Characterizing the Bioactive Ingredients in Sesame Oil Affected by Multiple Roasting Methods

El-Beltagi

Maraei

El-Ansary

et al. 2022

Foods

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“…Otherwise, some compounds have been identified here for the first time. With a molecular formula of C 27 H 30 O 15 (peak 15) at 10.21 min, the compound was identified as luteolin hexoside deoxyhexoside in agreement with its m/z in the source fragment 285 according to Mekky et al, who previously described it in sesame oil [ 40 ]. At 9.78 min (peak 14) with the m/z 717 and the predicted molecular formula of C 36 H 30 O 16 , the detected compound was proposed to be salvianolic acid as previously described in basil [ 38 ].…”

Section: Resultsmentioning

confidence: 89%

Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode

et al. 2023

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Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.

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“…The sample was ready at a concentration of 1 mg/mL in HPLC-grade methanol, and the injection volume was 10 µL. The metabolites characterization was according to the strategy proposed by several studies [ 47 , 49 , 89 ]. Metabolite assignments were achieved by the observation of the retention time (RT) for each candidate alongside the mass to charge ( m / z ) observed in the positive mode [M+H]+, molecular formula, mass score not less than 80%, error ± 3, and major MS/MS fragments.…”

Section: Methodsmentioning

confidence: 99%

Quality Control, Anti-Hyperglycemic, and Anti-Inflammatory Assessment of Colvillea racemosa Leaves Using In Vitro, In Vivo Investigations and Its Correlation with the Phytoconstituents Identified via LC-QTOF-MS and MS/MS

Hafeez

Gindi

Hetta

et al. 2022

Plants

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Colvillea racemosa is a cultivated ornamental plant that is a monotypic genus of Fabaceae. It is native to Madagascar, with limited studies. For the first time, the leaf quality control parameters, the anti-hyperglycemic and anti-inflammatory in vitro activity of Colvillea racemosa ethanol extract (CRE) and its fractions of petroleum ether (CRP), methylene chloride (CRMC), ethyl acetate (CREA), n-butanol (CRB), and methanol (CRME) were evaluated. It exhibited significant inhibition against α-amylase, α-glucosidase and membrane stabilization. CRB was the most active fraction, and in vivo studies revealed that oral treatment with CRB of STZ-induced diabetic rats efficiently lowered blood glucose by 67.78%, reduced serum nitric oxide and lipid peroxide levels by 41.23% and 38.45%, respectively, and increased the GSH level by 90.48%. In addition, compared with the diabetic group, there was a 52.2% decrease in serum VCAM, a 55.5% increase in paraoxonase, an improved lipid profile, and improved liver and kidney functions for a treated diabetic group with CRB. Metabolite profiling of CRB was determined by UPLC-ESI-QTOF-MS and tandem MS/MS. Twenty-three chromatographic peaks were identified, which were classified into phenolic compounds and amino acids. The characterized flavonoids were apigenin and luteolin derivatives.

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Metabolic Profiling of the Oil of Sesame of the Egyptian Cultivar ‘Giza 32’ Employing LC-MS and Tandem MS-Based Untargeted Method

Cited by 23 publications

References 45 publications

Characterizing the Bioactive Ingredients in Sesame Oil Affected by Multiple Roasting Methods

Characterizing the Bioactive Ingredients in Sesame Oil Affected by Multiple Roasting Methods

Optimization of the Extraction Conditions of Bioactive Compounds from Ocimum basilicum Leaves Using Ultrasound-Assisted Extraction via a Sonotrode

Quality Control, Anti-Hyperglycemic, and Anti-Inflammatory Assessment of Colvillea racemosa Leaves Using In Vitro, In Vivo Investigations and Its Correlation with the Phytoconstituents Identified via LC-QTOF-MS and MS/MS

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