Metabolic Properties of Mouse Uterine Endometrial Cells after Isolation with Collagenase

Buxton, Louise E; Murdoch, R. N.

doi:10.1071/bi9820277

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…The distended, everted horns were tied at the cervical ends, removed from the needles, and placed in HBSS containing 0.5% bacterial collagenase type I (Sigma Chemical Co.) and 1% bovine serum albumin (Armour Pharmaceutical Co., Kankakee, Ill.). Bovine serum albumin was added to minimize the effects of proteolytic enzymes contained in the collagenase on the cell surface (6). Collagenase solution (1 ml) was prepared for each uterine horn.…”

Section: Methodsmentioning

confidence: 99%

In vitro adherence of Escherichia coli to endometrial epithelial cells of rats and influence of estradiol

Nishikawa¹,

Baba²

1985

Infect Immun

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The influence of ovarian hormones on the adhesion of Escherichia coli to endometrial epithelial cells was investigated in an in vitro system. Endometrial cells liberated by collagenase from rat uteri were used. Optimal test conditions were obtained when 5 x 108 E. coli bacteria were added to 105 epithelial cells and incubated for 60 min. The adhesion of the organisms was inhibited by the addition of either mannose or a-methyl-Dmannopyranoside. When epithelial cells collected from uteri of estradiol-treated rats were used, the number of E. coli adhering to the cells was markedly lower than that adhering to epithelial cells collected from control rats. These results suggest that E. coli adheres to endometrial epithelial cells with so-called type 1 pili and that estradiol alters the nature of the endometrial epithelium and prevents the adherence of the organisms to the cells.

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Section: Methodsmentioning

confidence: 99%

In vitro adherence of Escherichia coli to endometrial epithelial cells of rats and influence of estradiol

Nishikawa¹,

Baba²

1985

Infect Immun

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Glycolysis in the mouse uterus during the early post‐implantation stages of pregnancy and the effects of acute doses of ethanol

Murdoch

1987

Teratology

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The objective of this study was to assess the possibility that, in mice, alcohol interferes with uterine glycolysis to influence indirectly embryonal development. Glycolysis was found to be a major metabolic process in the mouse uterus and its pattern of activity changed during post-implantation pregnancy between days 5 and 9. This was evidenced by changes in glycolytic enzymes and intermediates, and in the capacity of endometrial preparations to consume oxygen and produce lactate during incubation in vitro. The intraperitoneal administration of ethanol at 3.0 and 6.0 gm/kg body weight, but not at 1.5 gm/kg body weight, on day 9 of pregnancy modified glycolysis in the uterus after 2 hours, resulting in significant changes in the rates of accumulation of glucose, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-diphosphate, and citrate. These effects were not mediated by changes in redox state, since the ratio of lactate to pyruvate remained unchanged after injection of ethanol. Citrate was considered to be a major factor involved both in the regulation of uterine glycolysis and in the ethanol-induced alterations in the levels of hexose phosphates. The elevated levels of uterine glucose registered after treatment with ethanol were related to an acceleration of glycogen degradation in the liver and not in the uterine endometrium. The results suggest that part of the adverse action that these doses of ethanol exert on embryonal development stems from indirect effects that involve uterine functions relating to the nurture of the conceptus via glycolysis.

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Evidence for cross-talk between the LH receptor and LH during implantation in mice

Gridelet

Tsampalas

Berndt

et al. 2013

Reprod. Fertil. Dev.

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Abstract. The present study investigated the first interaction that occurs between the blastocyst and endometrium during implantation. Given the ethical objections to studying implantation in humans, a mouse model was used to study the dialogue between luteinising hormone (LH) and luteinising hormone receptor (LHCGR). Several studies performed on LHCGR-knockout mice have generated controversy regarding the importance of the dialogue between LH and LHCGR during implantation. There has been no demonstration of a bioactive LH-like signal produced by the murine blastocyst. The first aim of the present study was to examine and quantify, using radioimmunoassay, the generation of a bioactive LH signal by the murine blastocyst. We went on to examine and quantify endometrial Lhcgr expression to validate the mouse model. Expression of LHCGR in mouse uteri was demonstrated using immunohistochemistry and western blot analysis. To quantify the expression of Lh in the mouse blastocyst and Lhcgr in the endometrium, reverse transcription-polymerase chain reaction (RT-PCR) and real-time quantitative (q) RT-PCR were performed. The results demonstrate that Lhcgr expression in BALB/c mouse endometrial epithelium is increased at the time of implantation and indicate that LHCGR may contribute to the implantation process. In support of this hypothesis, we identified a bioactive LH signal at the time of murine blastocyst implantation.

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Metabolic Properties of Mouse Uterine Endometrial Cells after Isolation with Collagenase

Abstract: Uterine horns from unmated, pseudopregnant (days 1-5) and pregnant (days 4 and 5) mice were dissected and treated with collagenase (5 mg/ml) in a Ringer solution to isolate viable endometrial cells with maximal recovery of the luminal epithelium.

Cited by 3 publications

References 30 publications

In vitro adherence of Escherichia coli to endometrial epithelial cells of rats and influence of estradiol

In vitro adherence of Escherichia coli to endometrial epithelial cells of rats and influence of estradiol

Glycolysis in the mouse uterus during the early post‐implantation stages of pregnancy and the effects of acute doses of ethanol

Evidence for cross-talk between the LH receptor and LH during implantation in mice

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