Metabolic properties of Pachysolen tannophilus mutants producing xylitol and ethanol from D-xylose

Bolotnikova, O. I.; Meshcheryakova, O. V.; Mikhailova, N. P.; Гинак, А. И.

doi:10.1134/s0026261715040050

Cited by 2 publications

(2 citation statements)

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“…The metabolic selection criteria for either of these hydroxyl compounds are still under investigation. In another development, Bolotnikova, Meshcheryakova, Mikhailova, and Ginak (2015) 2014) investigated a repeated-batch approach for ethanol production by Celite immobilized P. tannophilus from glycerol involving the activities of mitochondria electron transport chain under limited and controlled aeration conditions. 6.2 and 5.8 g/L maximum ethanol concentration were obtained for unimmobilized and immobilized cells respectively which were low compared to the findings of Liu, Jensen, and Workman (2012) who obtained 28.1 g/L using crude glycerol, a byproduct of biodiesel transesterification as the substrate for the un-immobilized P. tannophilus fermentation.…”

Section: Introductionmentioning

confidence: 99%

“…The metabolic selection criteria for either of these hydroxyl compounds are still under investigation. In another development, Bolotnikova, Meshcheryakova, Mikhailova, and Ginak (2015) investigated the activities of xylose reductase (XR) and xylitol dehydrogenase (XDH) which catalyzed the synthesis of d ‐xylulose in mutant P. tannophilus strains resulting in the production of either xylitol or ethanol solvent. The xylitol‐producing strain exhibited low activities of XDH, XR with preferential affinity to NADPH/NAD + dependent malate dehydrogenase, and cytochrome c oxidase.…”

Section: Introductionmentioning

confidence: 99%

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Genetics and metabolic engineering of yeast strains for efficient ethanol production

Adebami

Kuila

Ajunwa

et al. 2021

J Food Process Engineering

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Bioethanol production from monomeric sugar is performed by several yeasts. But there are several limitations associated with yeast strains such as their low tolerance to ethanol, toxic inhibitors, and high sugar concentration. Genetic and metabolic engineering of potential yeast strains can overcome the above limitations. The present article summarized current genetic and metabolic engineering approaches for the development of yeast strain for efficient ethanol production. The review systematically examined bioethanol generations based on substrate utilization, criteria for strain selections, strategies for strain improvements including randomized mutagenesis, genetic engineering, metabolic engineering, genome editing, whole genome (re)sequencing, promoter engineering, quantitative trait locus analysis, protein engineering, and evolutionary engineering. Different fermentation technologies employed in hydrolysate fermentation including low gravity (LG), high gravity (HG), and very high gravity (VHG) as well as challenges of yeast strains development and its future prospect have been critically evaluated in this article. Significant engineering efforts are imminent for yeast‐based second‐generation biofuel to leave a demonstration phase through strain improvement and become economically competitive with fossil fuel. Practical Applications This is a comprehensive review of yeast strain development for bioethanol production. The readers should be able to acquire some basic knowledge on: The accompanied substrates for bioethanol generations as well as the technologies and challenges behind them. The criteria to consider in selecting yeast strain for bioengineering development. Different strategies and their reported applications employed in yeast strain development including randomized mutagenesis, genetic engineering, metabolic engineering, genome editing, whole genome (re)sequencing, promoter engineering, quantitative trait locus (QTL) analysis, protein engineering, and evolutionary engineering. Challenges and merits of different fermentation technologies employed in hydrolysate fermentation including LG, HG, and VHG. Possible challenges to encounter in developing yeast strain for bioethanol production. Desirable traits to consider in the selection and development of yeast strains for bioethanol production.

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Section: Introductionmentioning

confidence: 99%

“…The metabolic selection criteria for either of these hydroxyl compounds are still under investigation. In another development, Bolotnikova, Meshcheryakova, Mikhailova, and Ginak (2015) investigated the activities of xylose reductase (XR) and xylitol dehydrogenase (XDH) which catalyzed the synthesis of d ‐xylulose in mutant P. tannophilus strains resulting in the production of either xylitol or ethanol solvent. The xylitol‐producing strain exhibited low activities of XDH, XR with preferential affinity to NADPH/NAD + dependent malate dehydrogenase, and cytochrome c oxidase.…”

Section: Introductionmentioning

confidence: 99%