Metabolic Quotients of the Gravid Uterus of the Chronically Catheterized Guinea Pig

Block, Steven M.; Sparks, John W.; Johnson, Robert L.; Battaglia, Frederick C.

doi:10.1203/00006450-198508000-00013

Cited by 19 publications

(21 citation statements)

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“…One of the guinea pigs was studied on five occasions over a period of 8 days. Some of the data for animals of less than 50 days gestation have been reported previously (6). The characteristics of all of the study animals are presented in Table 1 , including gestational ages and the weights and number of fetuses.…”

Section: Resultsmentioning

confidence: 99%

“…For reasons related to the large mass of conceptus produced, pregnancy exerts a significant metabolic and cardiovascular stress on the female guinea pig (3)(4)(5)(6)(7). This is particularly true in late pregnancy when the uterus is approaching maximal size.…”

Section: Discussionmentioning

confidence: 99%

“…The in-enriched with vitamin C. At gestational ages ranging from 38 to crease in supply of substrates for the rapid growth the 55 days, the animals were anesthetized, and the systemic arterial conceptus toward the end of gestation is accounted for by and uterine venous circulations were catheterized using the surincreasing extraction of substrate and by greatly increasing gical and anesthetic methods previously described from this with increasing gestation. glucose re-laboratory (4,6,8). The method for catheterization of the uterine mains the major metabolic fuel of the guinea pig conceptus vein was modified in that the catheter material used was polyand acetate is consistently taken up by the uterus, these urethane (Microrenethane, Braintree Scientific, 0.034 OD x wbstrates for the fate 0.023 ID) rather than polyvinyl, in order to reduce clot formathe oxygen taken up and fall far short of the predicted tion.…”

mentioning

confidence: 99%

“…Recently, methodology for the chronic catheterization of (200 U/ml) and sealed. The animals were studied daily in this this species has been developed (3)(4)(5)(6)(7)(8), permitting metabolic and manner for as many days as possible. After the venous catheter cardiovascular studies in conscious, unstressed animals.…”

mentioning

confidence: 99%

“…The AV differences from the four sets of measurements were averaged, and the mean values were used as a single point for calculations of metabolic quotients on each study day. The metabolic quotients were calculated as the molar ratio of the AV substrate concentration difference divided by the AV oxygen concentration difference multiplied by a molar constant which is the number of mmoles of oxygen required to completely oxidize 1 mmol of substrate (6). The coefficients of extraction were calculated as:…”

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confidence: 99%

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Uterine Metabolism of the Pregnant Guinea Pig as a Function of Gestational Age

et al. 1988

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ABSTFWCX. During the last half of pregnancy, the fetal proximately constant until about 50 days, after which it increases guinea pig grows exponentially at 7.l%/day. In order to as a function of spontaneous fetal weight. A number of metabolic define the metabolic requirements of the gravid uterus changes also occur after that time, including alterations in blood during this period of rapid growth, catheters were placed substrate concentrations and glucose turnover rates (4, 9, 10). in the femoral artery and uterine vein of guinea pigs at The purpose of the present study is to determine gestational gestational ages ranging from 40 days to term (68 days). changes in metabolism of the gravid uterus in pregnant guinea The animals were studied in the unstressed state after full pigs under comparably unstressed conditions. The data obtained recovery from surgery and anesthesia. Arterial and venous in the present study for late gestation guinea pigs have been concentrations of oxygen, glucose, lactate, acetoacetate, 8-coupled with data reported previously for animals of less than hydroxybutyrate, and acetate were measured, and meta-50 days gestation (6). bolic quotients and coefficients of extraction were calculated. The glucose/oxygen quotient did not change signifi-METHODS cantly in the last half of pregnancy averaging 1.26, while the lactate/oxygen quotient remained unchanged at -0.42, Hartley strain guinea pigs were obtained from a commercial and the (glucose + lactate)/oxygen quotient remained un-breeder with pregnancy timed within one day by date of exposure changed at 0.82. Coefficients of extraction of glucose and to the male. ~~i~~l~ were fed ad libitum a solid guinea pig diet oxygen increased linearly with gestational age. The in-enriched with vitamin C. At gestational ages ranging from 38 to crease in supply of substrates for the rapid growth the 55 days, the animals were anesthetized, and the systemic arterial conceptus toward the end of gestation is accounted for by and uterine venous circulations were catheterized using the surincreasing extraction of substrate and by greatly increasing gical and anesthetic methods previously described from this with increasing gestation. glucose re-laboratory (4,6, 8). The method for catheterization of the uterine mains the major metabolic fuel of the guinea pig conceptus vein was modified in that the catheter material used was polyand acetate is consistently taken up by the uterus, these urethane (Microrenethane, Braintree Scientific, 0.034 OD x wbstrates for the fate 0.023 ID) rather than polyvinyl, in order to reduce clot formathe oxygen taken up and fall far short of the predicted tion. nutritional requirements of the fetus for both oxidative ~h~ animals were studied 48 to 72 h postoperative~y, allowing and growth-(Pediatr Res 23: [45][46][47][48][49] 1988) sufficient time for recovery from surgery. Daily food intake and blood substrate concentrations had returned to control levels in Abbreviations the study animals. Four sets of maternal arterial and uterine venou...

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Section: Resultsmentioning

confidence: 99%