Metabolic rate and level of activity determined in tilapia (Oreochromis mossambicus Peters) by direct and indirect calorimetry and videomonitoring

Ginneken, V.J.T. van; Addink, Albert D.F.; Thillart, Guido E.E.J.M. van den; Körner, F. J. S. W.; Noldus, L.P.J.J.; Buma, Marc

doi:10.1016/s0040-6031(96)03106-1

Cited by 36 publications

(23 citation statements)

References 35 publications

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“…This was at least partly explained by reductions in the resting ṀO 2 measured in normoxia ( Fig. 1), which has been shown to be related to P crit in many previous studies (van Ginneken et al, 1997;Mandic et al, 2009;Speers-Roesch et al, 2010). This supports the notion that low routine O 2 demands are associated with hypoxia tolerance, but there are clearly other changes in physiology with hypoxia acclimation that are also important (including those observed here).…”

Section: Discussionsupporting

confidence: 88%

“…cytochrome c oxidase, citrate synthase) (van den Thillart et al, 1980(van den Thillart et al, , 1994Almeida-Val et al, 1995;Martínez et al, 2006;Richards et al, 2008). In addition to mechanisms that help maintain ATP supply, some tolerant organisms can also reduce ATP demands through active depression of resting metabolic rate (van Waversveld et al, 1989;van Ginneken et al, 1997). This is associated with reductions in energetically costly processes, such as ion transport, protein synthesis and mitochondrial proton leak (Bickler and Buck, 2007;Wood et al, 2007Wood et al, , 2009De Boeck et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Distinct physiological strategies are used to cope with constant hypoxia and intermittent hypoxia in killifish (Fundulus heteroclitus)

Borowiec

Darcy

Gillette

et al. 2015

Journal of Experimental Biology

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Many fish encounter hypoxia on a daily cycle, but the physiological effects of intermittent hypoxia are poorly understood. We investigated whether acclimation to constant (sustained) hypoxia or to intermittent diel cycles of nocturnal hypoxia (12 h normoxia:12 h hypoxia) had distinct effects on hypoxia tolerance or on several determinants of O 2 transport and O 2 utilization in estuarine killifish. Adult killifish were acclimated to normoxia, constant hypoxia, or intermittent hypoxia for 7 or 28 days in brackish water (4 ppt). Acclimation to both hypoxia patterns led to comparable reductions in critical O 2 tension and resting O 2 consumption rate, but only constant hypoxia reduced the O 2 tension at loss of equilibrium. Constant (but not intermittent) hypoxia decreased filament length and the proportion of seawatertype mitochondrion-rich cells in the gills (which may reduce ion loss and the associated costs of active ion uptake), increased blood haemoglobin content, and reduced the abundance of oxidative fibres in the swimming muscle. In contrast, only intermittent hypoxia augmented the oxidative and gluconeogenic enzyme activities in the liver and increased the capillarity of glycolytic muscle, each of which should facilitate recovery between hypoxia bouts. Neither exposure pattern affected muscle myoglobin content or the activities of metabolic enzymes in the brain or heart, but intermittent hypoxia increased brain mass. We conclude that the pattern of hypoxia exposure has an important influence on the mechanisms of acclimation, and that the optimal strategies used to cope with intermittent hypoxia may be distinct from those for coping with constant hypoxia.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Distinct physiological strategies are used to cope with constant hypoxia and intermittent hypoxia in killifish (Fundulus heteroclitus)

Borowiec

Darcy

Gillette

et al. 2015

Journal of Experimental Biology

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“…Van Ginneken et al (1997) used EthoVision to combine calorimetry of tilapia with analysis of activity. In this way, the effects of various movements on the increased metabolic rate of the fish under various light and oxygen conditions could be analyzed.…”

Section: Fish Behaviormentioning

confidence: 99%

EthoVision: A versatile video tracking system for automation of behavioral experiments

Noldus

Spink

Tegelenbosch

2001

Behavior Research Methods, Instruments, & Computers

598

446

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“…calorimetric) measurements in goldfish (van Ginneken et al, 1994(van Ginneken et al, , 2004 and tilapia (van Ginneken et al, 1997) suggest that MRD may be employed at hypoxic O 2 tensions (Pw O2 for water). Indeed, goldfish reduced metabolic heat by ∼31% at ∼2.1 kPa Pw O2 compared with normoxia (∼21 kPa) (van Ginneken et al, 2004), and tilapia reduced metabolic heat by ∼40% at ∼1.1 kPa Pw O2 (van Ginneken et al, 1997). However, it is still unknown how these changes in metabolic heat correspond with changes in aerobic and anaerobic metabolism, and how this is affected by Pw O2 .…”

Section: Introductionmentioning

confidence: 99%

“…We used calorespirometry because it is the 'gold standard' of MR measurements and the only way to accurately measure MR on hypoxemic animals in real time (see Kaiyala and Ramsay, 2011;Nelson, 2016). Despite the superiority of calorespirometry, only a few studies have measured the metabolic heat of fishes (Addink et al, 1991;Regan et al, 2013;Stangl and Wegener, 1996;van Ginneken et al, 1994van Ginneken et al, , 1997van Ginneken et al, , 2004van Waversveld et al, 1989), and only three of these (van Ginneken et al, 1994(van Ginneken et al, , 1997(van Ginneken et al, , 2004 have measured metabolic heat at Pw O2 other than normoxia and anoxia. While the data from these studies suggest that MRD is employed at hypoxic Pw O2 values, they exposed their organisms to progressive hypoxia over sometimes prolonged periods of time and they did not relate their measurements to P crit nor directly assess the contributions of anaerobic metabolism at various hypoxic Pw O2 values.…”

Section: Introductionmentioning

confidence: 99%

Calorespirometry reveals that goldfish prioritize aerobic metabolism over metabolic rate depression in all but near-anoxic environments

Regan

Gill

Richards

2016

Journal of Experimental Biology

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Metabolic rate depression (MRD) has long been proposed as the key metabolic strategy of hypoxic survival, but surprisingly, the effects of changes in hypoxic O 2 tensions (Pw O2 ) on MRD are largely unexplored. We simultaneously measured the O 2 consumption rate (Ṁ O2 ) and metabolic heat of goldfish using calorespirometry to test the hypothesis that MRD is employed at hypoxic Pw O2 values and initiated just below P crit , the Pw O2 below which Ṁ O2 is forced to progressively decline as the fish oxyconforms to decreasing Pw O2 . Specifically, we used closed-chamber and flow-through calorespirometry together with terminal sampling experiments to examine the effects of Pw O2 and time on Ṁ O2 , metabolic heat and anaerobic metabolism (lactate and ethanol production). The closedchamber and flow-through experiments yielded slightly different results. Under closed-chamber conditions with a continually decreasing Pw O2 , goldfish showed a P crit of 3.0±0.3 kPa and metabolic heat production was only depressed at Pw O2 between 0 and 0.67 kPa. Under flow-through conditions with Pw O2 held at a variety of oxygen tensions for 1 and 4 h, goldfish also initiated MRD between 0 and 0.67 kPa but maintained Ṁ O2 to 0.67 kPa, indicating that P crit is at or below this Pw O2 . Anaerobic metabolism was strongly activated at Pw O2 ≤1.3 kPa, but only used within the first hour at 1.3 and 0.67 kPa, as anaerobic end-products did not accumulate between 1 and 4 h exposure. Taken together, it appears that goldfish reserve MRD for near-anoxia, supporting routine metabolic rate at sub-P crit Pw O2 values with the help of anaerobic glycolysis in the closed-chamber experiments, and aerobically after an initial (<1 h) activation of anaerobic metabolism in the flow-through experiments, even at 0.67 kPa Pw O2 .

show abstract

Metabolic rate and level of activity determined in tilapia (Oreochromis mossambicus Peters) by direct and indirect calorimetry and videomonitoring

Cited by 36 publications

References 35 publications

Distinct physiological strategies are used to cope with constant hypoxia and intermittent hypoxia in killifish (Fundulus heteroclitus)

Distinct physiological strategies are used to cope with constant hypoxia and intermittent hypoxia in killifish (Fundulus heteroclitus)

EthoVision: A versatile video tracking system for automation of behavioral experiments

Calorespirometry reveals that goldfish prioritize aerobic metabolism over metabolic rate depression in all but near-anoxic environments

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