Metabolic rate and oxidative stress in insects exposed to low temperature thermal fluctuations

Lalouette, Lisa; Williams, Carroll M.; Hervant, Frédéric; Sinclair, Brent J.; Renault, David

doi:10.1016/j.cbpa.2010.11.007

Cited by 200 publications

(172 citation statements)

References 46 publications

Supporting

Mentioning

160

Contrasting

Unclassified

Order By: Relevance

“…Chill injury and subsequent mortality have been associated with aberrations in ion homeostasis and metabolic imbalance in several insect species (Koštál, 2006;Koštál et al, 2007;Lalouette et al, 2011;Macmillan et al, 2012). Disturbances in ion homeostasis could help to explain some of the large-scale physiological effects seen in chill-injured insects such as abnormal muscle contraction (Yocum et al, 1994) and atypical neuronal function (Hosler et al, 2000).…”

Section: Ion and Metabolic Imbalance Resulting From Membrane Phase Trmentioning

confidence: 99%

“…In adult Alphitobius diaperinus, exposure to fluctuating temperatures during chilling has been associated with decreased levels of reactive oxygen species (ROS) when compared with individuals reared under constant low temperatures (Lalouette et al, 2011).…”

Section: Oxidative Stress As a Component Of Chill Injurymentioning

confidence: 99%

“…The downstream consequences of this phenomenon are likely to be complex, but disruption of ion homeostasis and metabolic imbalance are probable outcomes (Kostal, 2004;Koštál et al, 2006). Additionally, chill injury has been associated with the occurrence of oxidative stress (Lalouette et al, 2011;Rojas and Leopold, 1996).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptional responses to fluctuating thermal regimes underpinning differences in survival in the solitary bee Megachile rotundata

Torson

Yocum

Rinehart

et al. 2015

Journal of Experimental Biology

View full text Add to dashboard Cite

The transcriptional responses of insects to long-term, ecologically relevant temperature stress are poorly understood. Long-term exposure to low temperatures, commonly referred to as chilling, can lead to physiological effects collectively known as chill injury. Periodically increasing temperatures during long-term chilling has been shown to increase survival in many insects. However, the transcripts responsible for this increase in survival have never been characterized. Here, we present the first transcriptome-level analysis of increased longevity under fluctuating temperatures during chilling. Overwintering post-diapause quiescent alfalfa leafcutting bees (Megachile rotundata) were exposed to a constant temperature of 6°C, or 6°C with a daily fluctuation to 20°C. RNA was collected at two different time points, before and after mortality rates began to diverge between temperature treatments. Expression analysis identified differentially regulated transcripts between pairwise comparisons of both treatments and time points. Transcripts functioning in ion homeostasis, metabolic pathways and oxidative stress response were up-regulated in individuals exposed to periodic temperature fluctuations during chilling. The differential expression of these transcripts provides support for the hypotheses that fluctuating temperatures protect against chill injury by reducing oxidative stress and returning ion concentrations and metabolic function to more favorable levels. Additionally, exposure to fluctuating temperatures leads to increased expression of transcripts functioning in the immune response and neurogenesis, providing evidence for additional mechanisms associated with increased survival during chilling in M. rotundata.

show abstract

Section: Ion and Metabolic Imbalance Resulting From Membrane Phase Trmentioning

confidence: 99%

Section: Oxidative Stress As a Component Of Chill Injurymentioning

confidence: 99%

See 1 more Smart Citation

Transcriptional responses to fluctuating thermal regimes underpinning differences in survival in the solitary bee Megachile rotundata

Torson

Yocum

Rinehart

et al. 2015

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…desiccated and nondesiccated). Other physiological mechanisms implicated in cold injury without freezing include loss of membrane fluidity (Lee et al, 2006;Overgaard et al, 2008;Shreve et al, 2007), oxidative stress (Joanisse and Storey, 1996;Lalouette et al, 2011;Rojas and Leopold, 1996), loss of ion homeostasis (Kostál et al, 2004;Kostál et al, 2007), protein misfolding (Rinehart et al, 2007) and the induction of cell death pathways , any of which could potentially contribute to the differential mortality of genotypes across temperatures. At the lowest temperatures, we cannot rule out the possibility that inoculative freezing caused by ice crystal formation on the external cuticle of flies contributes to mortality (Lee, 2010).…”

Section: Discussionmentioning

confidence: 99%

Mortality from desiccation contributes to a genotype-by-temperature interaction for cold survival inDrosophila melanogaster

Kobey

Montooth

2012

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYSurvival at cold temperatures is a complex trait, primarily because of the fact that the physiological cause of injury may differ across degrees of cold exposure experienced within the lifetime of an ectothermic individual. In order to better understand how chill-sensitive insects experience and adapt to low temperatures, we investigated the physiological basis for cold survival across a range of temperature exposures from −4 to 6°C in five genetic lines of the fruit fly Drosophila melanogaster. Genetic effects on cold survival were temperature dependent and resulted in a significant genotype-temperature interaction for survival across cold temperature exposures that differ by as little as 2°C. We investigated desiccation as a potential mechanism of injury across these temperature exposures. Flies were dehydrated following exposures near 6°C, whereas flies were not dehydrated following exposures near −4°C. Furthermore, decreasing humidity during cold exposure decreased survival, and increasing humidity during cold exposure increased survival at 6°C, but not at −4°C. These results support the conclusion that in D. melanogaster there are multiple physiological mechanisms of cold-induced mortality across relatively small differences in temperature, and that desiccation contributes to mortality for exposures near 6°C but not for subzero temperatures. Because D. melanogaster has recently expanded its range from tropical to temperate latitudes, the complex physiologies underlying cold tolerance are likely to be important traits in the recent evolutionary history of this fruit fly. Supplementary material available online at

show abstract

“…Chemically reactive molecules containing oxygen, known as ROS (reactive oxygen species), are formed as a natural by-product of the normal metabolism of oxygen [59]. In ectothermic animals, ROS increases at low temperatures, causing oxidative damage to cells [60]. Methionine residues may act as catalytic antioxidants, protecting both the protein and other macromolecules [61].…”

Section: (A) Genetic Clines and Environmental Correlates Of Mitochondmentioning

confidence: 99%

Thermal adaptation and clinal mitochondrial DNA variation of European anchovy

et al. 2014

View full text Add to dashboard Cite

Natural populations of widely distributed organisms often exhibit genetic clinal variation over their geographical ranges. The European anchovy, Engraulis encrasicolus, illustrates this by displaying a two-clade mitochondrial structure clinally arranged along the eastern Atlantic. One clade has low frequencies at higher latitudes, whereas the other has an anti-tropical distribution, with frequencies decreasing towards the tropics. The distribution pattern of these clades has been explained as a consequence of secondary contact after an ancient geographical isolation. However, it is not unlikely that selection acts on mitochondria whose genes are involved in relevant oxidative phosphorylation processes. In this study, we performed selection tests on a fragment of 1044 bp of the mitochondrial cytochrome b gene using 455 individuals from 18 locations. We also tested correlations of six environmental features: temperature, salinity, apparent oxygen utilization and nutrient concentrations of phosphate, nitrate and silicate, on a compilation of mitochondrial clade frequencies from 66 sampling sites comprising 2776 specimens from previously published studies. Positive selection in a single codon was detected predominantly (99%) in the anti-tropical clade and temperature was the most relevant environmental predictor, contributing with 59% of the variance in the geographical distribution of clade frequencies. These findings strongly suggest that temperature is shaping the contemporary distribution of mitochondrial DNA clade frequencies in the European anchovy.

show abstract

Metabolic rate and oxidative stress in insects exposed to low temperature thermal fluctuations

Cited by 200 publications

References 46 publications

Transcriptional responses to fluctuating thermal regimes underpinning differences in survival in the solitary bee Megachile rotundata

Transcriptional responses to fluctuating thermal regimes underpinning differences in survival in the solitary bee Megachile rotundata

Mortality from desiccation contributes to a genotype-by-temperature interaction for cold survival inDrosophila melanogaster

Thermal adaptation and clinal mitochondrial DNA variation of European anchovy

Contact Info

Product

Resources

About