Metabolic response of Lactobacillus acidophilus exposed to amoxicillin

Guo, Yunchang; Liu, Xi; Lu, Ya-Ting; Xue, Ling; Mo, Yi-Yi; Yin, Chunli; Zhu, Hongjia; Zheng, Heng; Liu, Yonghong; Guo, Hongwei; Lu, Rigang; Su, Zhiheng; Song, Hui

doi:10.1038/s41429-022-00518-6

Cited by 8 publications

(2 citation statements)

References 31 publications

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“…Non-targeted metabonomic techniques are often employed to explore the unknown metabolites produced by bacteria [ 32 , 33 ]. To further reveal the metabolic differences, KEGG and distinct analyses were performed to determine the beneficial molecules and their concentrations based on metabonomic assays.…”

Section: Discussionmentioning

confidence: 99%

Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius

Yang,

Shang,

Zhang

et al. 2023

BMC Microbiol

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Background As substitutes for antibiotics, probiotic bacteria protect against digestive infections caused by pathogenic bacteria. Ligilactobacillus salivarius is a species of native lactobacillus found in both humans and animals. Herein, a swine-derived Ligilactobacillus salivarius was isolated and shown to colonize the ileal mucous membrane, thereby promoting nutritional digestion, absorption, and immunity. To evaluate its probiotic role, the entire genome was sequenced, the genetic information was annotated, and the metabolic information was analyzed. Results The phylogenetic relationship indicated that the bacteria was closer to L. salivarius MT573555.1 and MT585431.1. Functional genes included transporters, membrane proteins, enzymes, heavy metal resistance proteins, and putative proteins; metabolism-related genes were the most abundant. The six types of metabolic pathways secreted by L. salivarius were mainly composed of secretory transmembrane proteins and peptides. The secretory proteins of L. salivarius were digestive enzymes, functional proteins that regulate apoptosis, antibodies, and hormones. Non-targeted metabolomic analysis of L. salivarius metabolites suggested that ceramide, pyrrolidone- 5- carboxylic acid, N2-acetyl-L-ornithine, 2-ethyl-2-hydroxybutyric acid, N-lactoyl-phenylalanine, and 12 others were involved in antioxidation, repair of the cellular membrane, anticonvulsant, hypnosis, and appetite inhibition. Metabolites of clavaminic acid, antibiotic X14889C, and five other types of bacteriocins were identified, namely phenyllactic acid, janthitrem G, 13-demethyl tacrolimus, medinoside E, and tertonasin. The adherence and antioxidation of L. salivarius were also predicted. No virulence genes were found. Conclusion The main probiotic properties of L. salivarius were identified using genomic, metabonomic, and biochemical assays, which are beneficial for porcine feeding. Our results provided deeper insights into the probiotic effects of L. salivarius.

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Section: Discussionmentioning

confidence: 99%

Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius

Yang,

Shang,

Zhang

et al. 2023

BMC Microbiol

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“…Metabolomics is an "omics" technique and is a new branch of systems biology, which can provide a comprehensive insight into the response of organisms to stimulation of multiparameter metabolites and focuses on the changes in details of metabolic profiles of animals and humans (Wang et al, 2023). The advantages of ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) include high throughput, sensitivity and accuracy, which allow for the determination of low concentrations of cell and microbial metabolites (Guo et al, 2022;Li et al, 2023). Molecular docking aims to predict the role of small molecule ligand target binding sites (Li et al, 2022).…”

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confidence: 99%

UPLC/Q‐TOF–MS‐based metabolomics and molecular docking analysis of Bifidobacterium adolescentis exposure to levofloxacin

Feng,

Guo,

Wang

et al. 2024

Biomedical Chromatography

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Antibiotic‐associated diarrhea is a common adverse reaction caused by the widespread use of antibiotics. The decrease in probiotics is one of the reasons why antibiotics cause drug‐induced diarrhea. However, few studies have addressed the intrinsic mechanism of antibiotics inhibiting probiotics. To investigate the underlying mechanism of levofloxacin against Bifidobacterium adolescentis, we used a metabolomics mass spectrometry‐based approach and molecular docking analysis for a levofloxacin‐induced B. adolescentis injury model. The results showed that levofloxacin reduced the survival rate of B. adolescentis and decreased the number of B. adolescentis. The untargeted metabolomics analysis identified 27 potential biomarkers, and many of these metabolites are involved in energy metabolism, amino acid metabolism and the lipid metabolism pathway. Molecular docking showed that levofloxacin can bind with aminoacyl‐tRNA synthetase and lactic acid dehydrogenase. This result provides a novel insight into the mechanism of the adverse reactions of levofloxacin.

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Production of Polyvinyl Alcohol/Amoxicillin – Chitosan/Collagen Hybrid Bilayer Membranes for Regeneration of Gingival Tissues

Aydin,

Ulag,

Nouri

et al. 2024

Macromolecular Bioscience

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Periodontal diseases, if untreated, can cause gum recession and tooth root exposure, resulting in infection and irreversible damage. Traditional treatments using autologous grafts are painful and often result in postoperative complications. Scaffolds offer a less invasive alternative, promoting cell proliferation and healing without additional surgery, thus enhancing comfort for patients and doctors. This study developed Chitosan (Chit)/Collagen (Col) film surfaces and drug‐loaded Polyvinyl Alcohol (PVA)/Amoxicillin (AMX) nanofibers using solvent casting and electrospinning methods, respectively. The surfaces are characterized by scanning electron microscopy (SEM), mechanical testing, Fourier Transform Infrared Spectroscopy (FTIR), and differential scanning calorimetry (DSC). Biocompatibility and antimicrobial properties are assessed using NIH/3T3 fibroblast cells and bacterial cultures. SEM images confirmed the structural integrity of AMX‐loaded 13% PVA nanofibers, while FTIR analysis validated the compositional integrity of PVA/AMX nanofibers and Chit/Col film hybrid surfaces. Cell studies showed over 90% viability for Chit/Col film + PVA/AMX nanofiber hybrid bilayer membranes, confirming their biocompatibility. The antimicrobial assessment indicated that the Chit/Col film + PVA/AMX (0.2%) nanofiber hybrid bilayer membrane exhibited superior efficacy against Streptococcus mutans. These findings suggest that this hybrid bilayer membrane can enhance cell growth, promote proliferation, and enable controlled drug release, offering significant promise for regeneration of gingival tissues.

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Metabolic response of Lactobacillus acidophilus exposed to amoxicillin

Cited by 8 publications

References 31 publications

Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius

Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius

UPLC/Q‐TOF–MS‐based metabolomics and molecular docking analysis of Bifidobacterium adolescentis exposure to levofloxacin

Production of Polyvinyl Alcohol/Amoxicillin – Chitosan/Collagen Hybrid Bilayer Membranes for Regeneration of Gingival Tissues

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