Metabolic Response of Pleurotus ostreatus to Continuous Heat Stress

Yan, Zhiyu; Zhao, Ming; Wu, Xiangli; Jin-xia, Zhang

doi:10.3389/fmicb.2019.03148

Cited by 33 publications

(23 citation statements)

References 45 publications

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“…Metabolic pathways and HSPs are considered a primary focus and elaborated below. These two components are crucial in energy regulation and protein turnover, and hence for cell survival ( Yan et al, 2020 ). More widely, biosynthesis and regulation of metabolic pathways and HSPs are of importance in many applied fields such as agriculture, food biotechnology and medicine ( Ene et al, 2014 ; Lamoth, Juvvadi & Steinbach, 2016 ; Sarmiento, Peralta & Blamey, 2015 ).…”

Section: Survey Methodsology and Objectivementioning

confidence: 99%

Fungal survival under temperature stress: a proteomic perspective

Bakar

Karsani

Alias

2020

PeerJ

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Background Increases in knowledge of climate change generally, and its impact on agricultural industries specifically, have led to a greater research effort aimed at improving understanding of the role of fungi in various fields. Fungi play a key role in soil ecosystems as the primary agent of decomposition, recycling of organic nutrients. Fungi also include important pathogens of plants, insects, bacteria, domestic animals and humans, thus highlighting their importance in many contexts. Temperature directly affects fungal growth and protein dynamics, which ultimately will cascade through to affect crop performance. To study changes in the global protein complement of fungi, proteomic approaches have been used to examine links between temperature stress and fungal proteomic profiles. Survey methodology and objectives A traditional rather than a systematic review approach was taken to focus on fungal responses to temperature stress elucidated using proteomic approaches. The effects of temperature stress on fungal metabolic pathways and, in particular, heat shock proteins (HSPs) are discussed. The objective of this review is to provide an overview of the effects of temperature stress on fungal proteomes. Concluding remarks Elucidating fungal proteomic response under temperature stress is useful in the context of increasing understanding of fungal sensitivity and resilience to the challenges posed by contemporary climate change processes. Although useful, a more thorough work is needed such as combining data from multiple -omics platforms in order to develop deeper understanding of the factor influencing and controlling cell physiology. This information can be beneficial to identify potential biomarkers for monitoring environmental changes in soil, including the agricultural ecosystems vital to human society and economy.

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Section: Survey Methodsology and Objectivementioning

confidence: 99%

Fungal survival under temperature stress: a proteomic perspective

Bakar

Karsani

Alias

2020

PeerJ

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“…Metabolomic analysis was performed on P. ostreatus mycelia treated with heat (40 °C) for 0–48 h by LC–MS according to previously described methods [ 47 ]. All treatments included controls incubated at 28 °C.…”

Section: Methodsmentioning

confidence: 99%

Trehalose alleviates high‐temperature stress in Pleurotus ostreatus by affecting central carbon metabolism

et al. 2021

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Background Trehalose, an intracellular protective agent reported to mediate defense against many stresses, can alleviate high-temperature-induced damage in Pleurotus ostreatus. In this study, the mechanism by which trehalose relieves heat stress was explored by the addition of exogenous trehalose and the use of trehalose-6-phosphate synthase 1 (tps1) overexpression transformants. Results The results suggested that treatment with exogenous trehalose or overexpression of tps1 alleviated the accumulation of lactic acid under heat stress and downregulated the expression of the phosphofructokinase (pfk) and pyruvate kinase (pk) genes, suggesting an ameliorative effect of trehalose on the enhanced glycolysis in P. ostreatus under heat stress. However, the upregulation of hexokinase (hk) gene expression by trehalose indicated the involvement of the pentose phosphate pathway (PPP) in heat stress resistance. Moreover, treatment with exogenous trehalose or overexpression of tps1 increased the gene expression level and enzymatic activity of glucose-6-phosphate dehydrogenase (g6pdh) and increased the production of both the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) and glutathione (GSH), confirming the effect of trehalose on alleviating oxidative damage by enhancing PPP in P. ostreatus under heat stress. Furthermore, treatment with exogenous trehalose or overexpression of tps1 ameliorated the decrease in the oxygen consumption rate (OCR) caused by heat stress, suggesting a relationship between trehalose and mitochondrial function under heat stress. Conclusions Trehalose alleviates high-temperature stress in P. ostreatus by inhibiting glycolysis and stimulating PPP activity. This study may provide further insights into the heat stress defense mechanism of trehalose in edible fungi from the perspective of intracellular metabolism.

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“…Metabolic pathways and HSPs are considered a primary focus and elaborated below. These two components are crucial in energy regulation and protein turnover, and hence for cell survival (Yan et al 2020). More widely, biosynthesis and regulation of metabolic pathways and HSPs are of importance in many applied fields such as agriculture, food biotechnology and medicine (Ene et al 2014;Lamoth et al 2016;Sarmiento et al 2015).…”

Section: Effects Of Temperature Stress In Fungal Proteomesmentioning

confidence: 99%

Peer Review #2 of "Fungal survival under temperature stress: a proteomic perspective (v0.1)"

Geddes‐McAlister¹

2020

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Metabolic Response of Pleurotus ostreatus to Continuous Heat Stress

Cited by 33 publications

References 45 publications

Fungal survival under temperature stress: a proteomic perspective

Fungal survival under temperature stress: a proteomic perspective

Trehalose alleviates high‐temperature stress in Pleurotus ostreatus by affecting central carbon metabolism

Peer Review #2 of "Fungal survival under temperature stress: a proteomic perspective (v0.1)"

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