Metabolic risks of neonates at birth following in utero exposure to HIV-ART: the amino acid profile of cord blood

Phenylalanine metabolism and tetrahydrobiopterin bio-availability in COVID-19 and HIV

“…Sample preparation and LC-MS/MS analysis was done based on the methods described in detail by Moutloatse et al (2017) [ 18 ], with slight deviation. Briefly, a 50 μL serum sample was mixed with an ice cold 410 μL isotope mixture in methanol and acetonitrile (1:5 ratio), centrifuged (protein crash) and supernatant dried under nitrogen gas.…”

“…One μl sample was injected and analyzed with a 1200 series Agilent HPLC system linked to an Agilent 6410 MSMS system. The settings for the LC-MS/MS were identical to those of Moutloatse et al (2017) [ 18 ].…”