Metabolic shift of polyphosphate-accumulating organisms with different levels of polyphosphate storage

Acevedo, B.; Oehmen, Adrian; Carvalho, Gilda; Seco, A.; Borrás, L.; Barat, R.

doi:10.1016/j.watres.2012.01.003

Cited by 163 publications

(87 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies suggested that these two clades used the glycolytic and TCA cycle differently under anaerobic conditions (Wexler et al, 2009;Oehmen et al, 2010a;Acevedo et al, 2012). Although we did not detect any apparent genome-level differences, we did note instances where the gene copy number for a particular enzyme important in the TCA cycle varied, which could influence expression levels.…”

Section: Discussioncontrasting

confidence: 66%

“…One bioreactor revealed enhanced TCA cycle gene expression aerobically, whereas the other showed enhanced synthesis anaerobically, suggesting that these two Accumulibacter populations had distinct anaerobic and aerobic metabolisms. Another study explored the impact of polyphosphate content on anaerobic performance and stoichiometry, and found that when polyphosphate content decreased in the cells, Clade IIA appeared to switch to a glycogen-accumulating metabolism, which no longer assisted in removing phosphate from the system (Acevedo et al, 2012). These findings suggest that distinct Accumulibacter clades inhabit different niches in EBPR ecosystems, each providing an important role in ecosystem function.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative genomics of two ‘Candidatus Accumulibacter’ clades performing biological phosphorus removal

et al. 2013

View full text Add to dashboard Cite

Members of the genus Candidatus Accumulibacter are important in many wastewater treatment systems performing enhanced biological phosphorus removal (EBPR). The Accumulibacter lineage can be subdivided phylogenetically into multiple clades, and previous work showed that these clades are ecologically distinct. The complete genome of Candidatus Accumulibacter phosphatis strain UW-1, a member of Clade IIA, was previously sequenced. Here, we report a draft genome sequence of Candidatus Accumulibacter spp. strain UW-2, a member of Clade IA, assembled following shotgun metagenomic sequencing of laboratory-scale bioreactor sludge. We estimate the genome to be 80–90% complete. Although the two clades share 16S rRNA sequence identity of >98.0%, we observed a remarkable lack of synteny between the two genomes. We identified 2317 genes shared between the two genomes, with an average nucleotide identity (ANI) of 78.3%, and accounting for 49% of genes in the UW-1 genome. Unlike UW-1, the UW-2 genome seemed to lack genes for nitrogen fixation and carbon fixation. Despite these differences, metabolic genes essential for denitrification and EBPR, including carbon storage polymer and polyphosphate metabolism, were conserved in both genomes. The ANI from genes associated with EBPR was statistically higher than that from genes not associated with EBPR, indicating a high selective pressure in EBPR systems. Further, we identified genomic islands of foreign origins including a near-complete lysogenic phage in the Clade IA genome. Interestingly, Clade IA appeared to be more phage susceptible based on it containing only a single Clustered Regularly Interspaced Short Palindromic Repeats locus as compared with the two found in Clade IIA. Overall, the comparative analysis provided a genetic basis to understand physiological differences and ecological niches of Accumulibacter populations, and highlights the importance of diversity in maintaining system functional resilience.

show abstract

Section: Discussioncontrasting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Comparative genomics of two ‘Candidatus Accumulibacter’ clades performing biological phosphorus removal

et al. 2013

View full text Add to dashboard Cite

show abstract

“…As shown in Figure 5, the COD removal efficiency in anaerobic tank was significantly higher than that in other tanks, accounting for 85.9% of total COD removal rate (92.5%) in the BNR-IC system, which is agreement well with the characteristic of DPAO (Acevedo et al 2012). In anaerobic condition, carbon source, especially VFA, was taken up by DPAO and stored them as PHB, resulting in the P release from the cells of DPAO.…”

Section: Performance Of Bnr-ic Process In Each Stagesupporting

confidence: 68%

A Novel Process for Nutrients Removal and Phosphorus Recovery From Domestic Wastewater by Combining BNR With Induced Crystallization

Lü

2014

Journal of Environmental Engineering and Landscape Management

View full text Add to dashboard Cite

Abstract. Trinitrotoluene (TNT), a commonly used explosive for military and industrial applications, can cause serious environmental pollution. 28-day laboratory pot experiment was carried out applying bioaugmentation using laboratory selected bacterial strains as inoculum, biostimulation with molasses and cabbage leaf extract, and phytoremediation using rye and blue fenugreek to study the effect of these treatments on TNT removal and changes in soil microbial community responsible for contaminant degradation. Chemical analyses revealed significant decreases in TNT concentrations, including reduction of some of the TNT to its amino derivates during the 28-day tests. The combination of bioaugmentation-biostimulation approach coupled with rye cultivation had the most profound effect on TNT degradation. Although plants enhanced the total microbial community abundance, blue fenugreek cultivation did not significantly affect the TNT degradation rate. The results from molecular analyses suggested the survival and elevation of the introduced bacterial strains throughout the experiment. Keywords: TNT, bioaugmentation, biostimulation, phytoremediation, microbial community.

show abstract

“…PAOs/DPAOs can use poly-P and/or glycogen to supply energy, while GAOs/DGAOs have no poly-P pool and so can only use glycogen to supply energy [22]. EPS are a potential energy supply for organisms in starvation situations [6].…”

Section: Proposed Mechanism Of Microorganism Spatial Distribution mentioning

confidence: 99%

“…GAOs therefore may have a tendency to use EPS rather than PAOs in a starvation scenario, and tend to crush from granules in situations where EPS are consumed as an energy and carbon source for GAOs aggregation [22].…”

Section: Proposed Mechanism Of Microorganism Spatial Distribution mentioning

confidence: 99%

Comparison of performance, microorganism populations, and bio-physiochemical properties of granular and flocculent sludge from denitrifying phosphorus removal reactors

Wang

Jiang

Hong

et al. 2015

Chemical Engineering Journal

View full text Add to dashboard Cite

Comparison of performance, microorganism populations, and bio-physiochemical properties of granular and flocculent sludge from denitrifying phosphorus removal reactors, Chemical Engineering Journal (2014), doi: http://dx.doi.org/10.1016/j.cej. 2014.09.065 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ABSTRACT:Granule formation frequently occurs in denitrifying phosphorus (P) removal systems.The differences between granules and flocculants, including P removal capacities, bio-physiochemical properties, and microorganism distribution and diversity, are however still poorly understood. Physical and biochemical characteristics of granules and sludge flocs were investigated through two laboratory-scale sequencing batch reactors (SBRs). One reactor was operated as a flocculent SBR and the other 2 was operated as a granular SBR, using a granule diameter of 1.92 ± 0.78 mm. Granular sludge had a higher tolerance to inhibitors (nitrite/free nitrite acids (FNAs)), and a higher percentage of P accumulating organisms (PAOs) (72 % vs. 64 % in flocs) than flocculent sludge. The granule crush tests and higher PAOII (unable to use nitrate as an electron acceptor) to PAOs ratios (over 72 %) by fluorescent in situ hybridization showed that in both reactors, glycogen accumulating organisms (GAOs) were mainly responsible for nitrate to nitrite reduction, and PAOII further reduced nitrite to nitrogen gas in association with anoxic P uptake; GAOs wash-out weakened the mutual relationship between GAOs and PAOII to some extent, which made denitrification of nitrate to nitrite inefficient and weakened subsequent anoxic P removal. GAOs existed mainly on the surface of the granules, whereas PAOs (PAOI+PAOII ) were distributed both on the surface and in the interior of the granules. Thus, GAOs had easier access to carbon sources but were at risk of suffering from exposure to FNA.

show abstract

Metabolic shift of polyphosphate-accumulating organisms with different levels of polyphosphate storage

Abstract: ElsevierAcevedo Juárez, B.; Camiña, C.; Corona, JE.; Borrás Falomir, L.; Barat Baviera, R. (2015). The metabolic versatility of PAOs as an opportunity to obtain a highly P-enriched stream for further P-recovery. Chemical Engineering Journal. 270:459-467. doi:10.1016/j.cej.2015

Cited by 163 publications

References 53 publications

Comparative genomics of two ‘Candidatus Accumulibacter’ clades performing biological phosphorus removal

Comparative genomics of two ‘Candidatus Accumulibacter’ clades performing biological phosphorus removal

A Novel Process for Nutrients Removal and Phosphorus Recovery From Domestic Wastewater by Combining BNR With Induced Crystallization

Comparison of performance, microorganism populations, and bio-physiochemical properties of granular and flocculent sludge from denitrifying phosphorus removal reactors

Contact Info

Product

Resources

About